COBRE: RIH: CORE B: PROTEOMICS: PROTEIN BIOINFORMATICS, HU GENOME, DNA

COBRE：RIH：核心 B：蛋白质组学：蛋白质生物信息学、HU 基因组、DNA

• 批准号: 8359711
• 负责人: DJURO JOSIC
• 金额: $ 57.02万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8359711
• 关键词: Bioinformatics; Biological Markers; Biological Process; Centers of Research Excellence; Chromatography; Complex; Core Facility; DNA; Disease; Electrophoresis; Funding; Genome; Grant; Hospitals; Institution; Ligands; Liquid Chromatography; Longevity; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Methods; Modification; NCI Center for Cancer Research; National Center for Research Resources; Peptides; Principal Investigator; Process; Protein Analysis; Proteins; Proteomics; Publications; Recording of previous events; Relative (related person); Research; Research Infrastructure; Research Methodology; Resources; Rhode Island; Role; Sampling; Services; Source; Translations; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; Universities; Western Blotting; cost; instrumentation; method development; programs; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Proteomics Core in the COBRE Center for Cancer Research Development (CCRD) at Rhode Island Hospital was established in September 2002 as part of the COBRE grant. The Core is currently offering LC-MS/MS and SELDI-TOF protein identification, biomarker identification with LC-MS/MS and SELDI-TOF mass spectrometry and 2-D electrophoresis, and chromatographic and 1- and 2-D electrophoretic separations and analysis of proteins and peptides as a service for four Rhode Island COBRE Centers, Rhode Island Hospital and other Lifespan Hospitals, Brown University and other neighboring institutions. Proteomics Core serves the following functions: 1) Identification of proteins isolated by 1- and 2-D gel electrophoresis, western blotting or chromatography. 2) Differential analysis and identification and relative quantification of up- or down-regulated proteins between two different samples with LC-MS/MS, SELDI-TOF MS and/or 2-D electrophoresis. 3) Study of protein-protein and protein-ligand interaction by use of liquid chromatography, SELDI-TOF MS and immunochemical methods combined with analyses with 1- and 2-D electrophoresis. 4) Isolation and identification of proteins associated in complexes to elucidate their biological function. 5) Identification and mapping of post-translation modifications and processing of target proteins. This renewal application describes: A) Specific Aim; B) History of the Proteomics Core facility, current instrumentation and operational plan for the facility; C) Core Progress including the role of the Proteomics Core had in fulfilling research objectives in COBRE-funded, NIH-funded and other research programs in Greater Providence and Rhode Island; Publications and other highlights; D) Experimental plan  to research the method development and identification of biomarkers for cancer and other diseases as well as plans and activities to obtain additional funding for the facility.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 罗德岛医院癌症研究开发中心（CCRD）的蛋白质组学核心成立于2002年9月，作为索贝尔赠款的一部分。该核心目前正在提供LC-MS/MS和SELDI-TOF蛋白识别，具有LC-MS/MS的生物标志物鉴定以及SELDI-TOF质谱和2-D电泳，以及色谱以及1-和2-D电泳分离以及分析蛋白质和肽作为四个罗德岛州鞋垫中心，罗德岛医院和其他寿命医院，布朗大学和其他邻近机构的服务。 蛋白质组学核心具有以下功能：1）通过1-和2-D凝胶电泳，蛋白质印迹或色谱分离的蛋白质鉴定。 2）具有LC-MS/MS，SELDI-TOF MS和/或2-D电泳的两个不同样品之间向上或下调的蛋白质的差异分析和鉴定和相对定量。 3）研究蛋白质蛋白质和蛋白质 - 配体的相互作用，使用液相色谱，塞尔德-TOF MS和免疫化学方法与1-和2-D电泳的分析结合使用。 4）分离和鉴定在复合物中相关的蛋白质以阐明其生物学功能。 5）识别和映射翻译后修饰和靶蛋白的处理。此续订应用程序描述： a）特定目标； b）蛋白质组学核心设施的历史，当前的工具和设施的运营计划； c）核心进步，包括蛋白质组学核心在大普罗维登斯和罗德岛州的COBRE资助，NIH资助和其他研究计划中实现研究目标方面的作用；出版物和其他亮点； d）研究方法开发和鉴定癌症和其他疾病的生物标志物的实验计划，以及计划和活动，以获得该设施的额外资金。