A Nanofluidic Nanoplasmonic Platform for Multiplexing Detection of Cancer Biomark

用于癌症生物标志物多重检测的纳流体纳米等离子体平台

• 批准号: 8003191
• 负责人: Jianjun Wei
• 金额: $ 17.94万
• 依托单位: CFD RESEARCH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8003191
• 关键词: Area; Arts; Basic Science; Benchmarking; Binding; Biochemical; Biological; Biological Assay; Biological Markers; Biological Monitoring; Biomedical Research; Body Fluids; Cancer Detection; Clinic; Clinical; Coupling; Detection; Development; Devices; Diagnostic; Disease; Drug Design; Early Diagnosis; Elements; Evaluation; Event; FDA approved; Film; Fluorescence; Gold; Government; Healthcare; Industry; Label; Liquid substance; Malignant Neoplasms; Malignant neoplasm of prostate; Measures; Medical; Metals; Methods; Microfluidic Microchips; Microfluidics; Miniaturization; Nanostructures; Nature; Noise; Optics; Persons; Pharmaceutical Preparations; Pharmacologic Substance; Phase; Physics; Physiological; Process; Prostate-Specific Antigen; Proteins; Proteomics; Protocols documentation; Reagent; Refractive Indices; Research; Residual state; Saliva; Sampling; Screening for cancer; Screening procedure; Serum; Shapes; Signal Transduction; Silver; Sputum; Structure; Study Section; Surface; Surface Plasmon Resonance; System; Technology; Testing; Thick; Time; Translations; United States National Aeronautics and Space Administration; Universities; Urine; Validation; Width; analytical tool; base; cancer diagnosis; clinical Diagnosis; clinical application; cost; design; drug discovery; improved; light intensity; meetings; micro-total analysis system; nano; nanochannel; nanofluidic; nanostructured; next generation; novel; novel strategies; phase 1 study; plasmonics; prognostic; protein transport; prototype; public health relevance; seal; self diagnosis; sensor; simulation; success; tool; transmission process

DESCRIPTION (provided by applicant): This project aims to develop a novel nanofluidic-nanoplasmonic platform to realize multiplexed monitoring of biological binding processes, specifically for detection of cancer biomarkers in bio-fluids. In contrast to current large-sized, cumbersome surface plasmon resonance (SPR) sensing technology, the proposed device is comprised of a multilayer nanostructured array that combines the functions of nanofluidics for effective reagent transport and nanoplasmonics for sensing, concurrently. The array is designed in such a way as to permit significantly enhanced Extraordinary Optical Transmission (EOT) with a primary peak in the NIR range (700- 1200 nm), with the transmission and spectra being determined by the surface plasmons (SP) manipulated in the embedded metal film. The array structure readily interfaces with microfluidic channels, making it amenable to highly parallel throughput screening in a lab-on-chip device. The new platform offers greater throughput compatibility, 5-10X enhanced sensitivity of refractive index changes compared to current grating SPR sensor, improved efficacy of analyte transport, significantly increased EOT intensity with NIR range spectra for favorable signal-to-noise detection, lower cost, and rapid turnaround times - benefiting early detection of biomarkers and other applications in healthcare and biomedical research. The Phase I study seeks to develop (design, fabricate, and test) a prototype of the nano-fluidic-plasmonics array integrated in a microfluidic channel, to adapt protocols for nano-confined flow-through transport validation and to culminate with a clear demonstration of improved plasmonic sensing of biomarkers. The nanostructure arrays and device optimization as well as integration with sample handling microfluidics for detecting multiple biomarkers in real biofluids are planned for Phase II. A multi-disciplinary partnership with expertise in SPR sensors and BioMEMS/nanofluidics (CFDRC), nanoplasmonics and nanophotonics (University of Pittsburgh), and disease proteomics (diagnostic/prognostic biomarkers) (UCLA) has been formed. PUBLIC HEALTH RELEVANCE: A clear need exists for portable, label-free, high throughput analytical tools that are suited for early detection of cancer biomarkers and related biological species in bio-fluids at trace amount levels, not only in medical applications (clinical or self-diagnosis) but also in biomedical research (proteomics, drug design and evaluation). The overall objective of this project is to develop a nano-fluidic-plasmonics-based sensing platform which can be readily integrated with microfluidics devices, and enable in-parallel transmission SPR sensing technology and lab-on-chip technology (sample separation, mixing, dilution, etc) for developing next generation nanoplasmonics-based bioanalytical tools that are capable of multiplexing differentiation of biomarkers as well as high parallel throughput studies.

描述（由申请人提供）：该项目旨在开发一种新型的纳米流体纳米质质平台，以实现对生物结合过程的多重监测，特别是用于检测生物流体中的癌症生物标志物。与当前的大型，繁琐的表面等离子体共振（SPR）传感技术相反，所提出的设备由多层纳米结构阵列组成，该阵列结合了纳米流通液的功能，用于有效的试剂传输和纳米浆料，以同时感测。该阵列的设计方式是可以显着增强非凡的光学传输（EOT），其主要峰在NIR范围内（700-1200 nm），其透射率和光谱由在嵌入式金属膜中操纵的表面等离子体（SP）确定。该阵列结构很容易与微流体通道接口，从而可以在实验室片上的芯片设备中进行高度平行的吞吐量筛选。 The new platform offers greater throughput compatibility, 5-10X enhanced sensitivity of refractive index changes compared to current grating SPR sensor, improved efficacy of analyte transport, significantly increased EOT intensity with NIR range spectra for favorable signal-to-noise detection, lower cost, and rapid turnaround times - benefiting early detection of biomarkers and other applications in healthcare and biomedical research.第一阶段的研究旨在开发（设计，制造和测试）在微流体通道中集成的纳米 - 富集式 - 泛质阵列的原型，以适应纳米限制的流通传输验证的协议，并清楚地证明了对生物标志物的血清素水平传感的明确证明。纳米结构阵列和设备优化以及与样品处理微流体的集成，用于检测实际生物流体中多种生物标志物的样品。与SPR传感器/纳米流体技术（CFDRC），纳米浆果和纳米光子学（匹兹堡大学）（匹兹堡大学）以及疾病蛋白质组学（诊断/预后生物标志物）（UCLA）的多学科合作伙伴关系。 公共卫生相关性：存在明确的需求，需要便携式，无标签，高通量分析工具，这些工具适用于以痕量水平（临床或自我诊断）的生物融合物中的癌症生物标志物和相关生物物种的早期检测，还适用于生物医学研究（蛋白质组学，药物设计和评估）。该项目的总体目的是开发基于纳米流体 - 气质的传感平台，该平台可以很容易地与微流体设备整合在一起，并启用并行的传输传输SPR感应技术和芯片上的芯片技术（样品分离，混合，稀释，稀释等），以开发基于下一代NanoPlasmonics Asportive and Mulldelive and Mulldix and squants y Mulldelive and Multibles的工具，该工具与多样化的工具相互作用。研究。