Animal Resource and Pathology Core

动物资源和病理学核心

• 批准号: 7514465
• 负责人: JAMES G FOX
• 金额: $ 20.65万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7514465
• 关键词: Animal Husbandry; Animals; Caring; Comparative Pathology; Containment; Controlled Environment; Embryo Transfer; Ensure; Equipment; Gene Silencing; Germ-Free; Hazardous Chemicals; Histology; Histopathology; Housing; Human Resources; Immunohistochemistry; Institutes; Laboratories; Medicine; Microinjections; Molecular; Mouse Strains; Nitrites; Pathogenesis; Pathology; Phenotype; Process; Program Research Project Grants; RNA Interference; Research; Research Personnel; Role; Safety; Services; Specific Pathogen Frees; System; Technology; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Work; animal facility; animal resource; animal tissue; carcinogenesis; comparative; containment equipment; embryonic stem cell; germ free condition; in vivo; man; molecular pathology; mouse model; programs; square foot

Core 2 is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The Pl.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 175,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-free work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project. Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in the Program Project Grant. The specific aims are as follows: Specific Aim #1 To provide histology, immunohistochemistry, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program Specific Aim #2 To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific Aim #3 Provide colony management of mouse models for use by program project Pi's and as needed generate transgenic animals by pronuclear microinjection of DMAconstructs, embryonic stem cell manipulation, or gene silencing using RNAi technology.

Core 2对于用于进行体内进行的最佳兽医护理和设施至关重要 实验。该计划项目的Pl。将可以访问比较医学的部门 AAAALAC认可的，中央管理和经营的175,000平方英尺的空间，位于五座建筑物中 研究所。在过去的八年中，每个单元都已建成或进行了广泛翻新。其中四个 建筑物有三个走廊系统，三个具有专门的生物接收单元，用于感染或 危险化学实验。住房特定病原体无病原体小鼠的障碍设施 也可以使用殖民地。这些动物设施为麻省理工学院调查人员提供了受控环境 用于进行生物医学相关研究的必要空间，设备和遏制设施 包括无菌作品在内的动物，还可以在实验过程中确保人员安全。这 DCM比较病理学实验室还将为人员和专业知识提供组织学 以及对从体内研究进行的动物组织的免疫细胞化学分析 程序项目的框架。特别是核心2的作用是提供设施，设备和 具有专业知识的人员，可以集中管理和监督畜牧业和实验动物 操纵并提供参与研究中进行的组织病理学和转基因服务 计划项目赠款。具体目的如下： 具体目的1提供组织学，免疫组织化学，病理学和分子表征 广泛表型和评估小鼠病理损害的专业知识 该程序中使用的模型 特定的目标＃2通过胚胎转移重新生动转基因和其他专业小鼠菌株以确保 该程序中使用的宝石的特定无病原体状态 特定目的＃3提供了鼠标模型的殖民地管理，以供程序PI和AS 需要通过对Dmaconstructs的小核显微注射来产生转基因动物， 胚胎干细胞操纵或使用RNAi技术的基因沉默。