Core A: Mouse Core

核心A：鼠标核心

基本信息

批准号：
10328099
负责人：
Mark S Anderson
金额：
$ 24.11万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-02-17 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10328099
关键词：
Animal Husbandry Animals Autoimmune Autoimmune Diseases Autoimmunity Biological Process Breeding Caring Complex Diabetes Mellitus Diabetic mouse Ensure Environment Generations Genetic Housing Immune Tolerance Immune system Inbred NOD Mice Incidence Insulin-Dependent Diabetes Mellitus Knock-out Maintenance Modeling Mouse Strains Mus Reporter Research Research Personnel STAT3 gene Transgenic Organisms Variant animal facility experience gain of function genetic makeup genetic strain improved member novel pathogen programs tool

Animal Husbandry Animals Autoimmune Autoimmune Diseases Autoimmunity Biological Process Breeding Caring Complex Diabetes Mellitus Diabetic mouse Ensure Environment Generations Genetic Housing Immune Tolerance Immune system Inbred NOD Mice Incidence Insulin-Dependent Diabetes Mellitus Knock-out Maintenance Modeling Mouse Strains Mus Reporter Research Research Personnel STAT3 gene Transgenic Organisms Variant animal facility experience gain of function genetic makeup genetic strain improved member novel pathogen programs tool

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项目摘要

STAT3 Variants as a Rheostat of Immune Tolerance Mouse Core Project Summary The use of genetically altered mice has revolutionized the study of complex biological processes, including immune tolerance and autoimmunity. All the projects in this P01 rely heavily on the use of genetically modified transgenic, knockout, and autoimmune-prone mouse strains. Many autoimmune prone and immunodeficient lines require specific expertise and conditions for optimal research, including housing in pathogen-free environments. Additionally, breeding and care of such animals can become complex when dealing with multiple genetically modified lines and different genetic strains of mice. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. Because of the reliance of several P01 projects on the NOD mouse model of diabetes and the novel STAT3 gain of function (GOF) mouse lines, much of the Core efforts will revolve around the maintenance, distribution and use of these lines in combination with other relevant immune system reporters or immunodeficient lines. In this way, the Core can help ensure the quality, genetic lineage, and sharing of animals needed for the collaborative studies of the P01 investigators.

STAT3变体是免疫耐受小鼠核心的阻尼斯特项目摘要遗传改变的小鼠的使用已彻底改变了对复杂生物过程的研究，包括免疫耐受性和自身免疫性。 P01中的所有项目都严重依赖于转基因修改的使用转基因，基因敲除和自身免疫性的小鼠菌株。许多自身免疫性容易发生和免疫缺陷线条需要特定的专业知识和最佳研究条件，包括无病原体中的住房环境。此外，处理这些动物的繁殖和护理可能会变得复杂多种遗传修饰的线和小鼠的不同遗传菌株。设想鼠标核心将通过在遗传复杂线的生成和维护中帮助简化和提高效率一个健康的无病原体环境。由于点头鼠标上的几个P01项目的依赖糖尿病模型和新型STAT3功能增益（GOF）小鼠线，许多核心工作将会围绕这些线的维护，分配和使用以及其他相关免疫系统记者或免疫缺陷线。这样，核心可以帮助确保质量，遗传谱系，并共享P01研究人员的合作研究所需的动物。