Core A: Mouse Core

核心A：鼠标核心

• 批准号: 10328099
• 负责人: Mark S Anderson
• 金额: $ 24.11万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-17 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10328099
• 关键词: Animal Husbandry; Animals; Autoimmune; Autoimmune Diseases; Autoimmunity; Biological Process; Breeding; Caring; Complex; Diabetes Mellitus; Diabetic mouse; Ensure; Environment; Generations; Genetic; Housing; Immune Tolerance; Immune system; Inbred NOD Mice; Incidence; Insulin-Dependent Diabetes Mellitus; Knock-out; Maintenance; Modeling; Mouse Strains; Mus; Reporter; Research; Research Personnel; STAT3 gene; Transgenic Organisms; Variant; animal facility; experience; gain of function; genetic makeup; genetic strain; improved; member; novel; pathogen; programs; tool

STAT3 Variants as a Rheostat of Immune Tolerance Mouse Core Project Summary The use of genetically altered mice has revolutionized the study of complex biological processes, including immune tolerance and autoimmunity. All the projects in this P01 rely heavily on the use of genetically modified transgenic, knockout, and autoimmune-prone mouse strains. Many autoimmune prone and immunodeficient lines require specific expertise and conditions for optimal research, including housing in pathogen-free environments. Additionally, breeding and care of such animals can become complex when dealing with multiple genetically modified lines and different genetic strains of mice. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. Because of the reliance of several P01 projects on the NOD mouse model of diabetes and the novel STAT3 gain of function (GOF) mouse lines, much of the Core efforts will revolve around the maintenance, distribution and use of these lines in combination with other relevant immune system reporters or immunodeficient lines. In this way, the Core can help ensure the quality, genetic lineage, and sharing of animals needed for the collaborative studies of the P01 investigators.

STAT3 变体作为免疫耐受小鼠核心变阻器 项目概要 转基因小鼠的使用彻底改变了复杂生物过程的研究，包括 免疫耐受和自身免疫。本 P01 中的所有项目都严重依赖转基因的使用 转基因、基因敲除和自身免疫倾向小鼠品系。许多自身免疫倾向和免疫缺陷 品系需要特定的专业知识和条件才能进行最佳研究，包括在无病原体的环境中进行饲养 环境。此外，在处理这些动物时，饲养和护理这些动物可能会变得复杂 多个转基因品系和不同基因品系的小鼠。预计鼠标核心将 帮助简化和提高遗传复杂品系的生成和维护效率 健康、无病原体的环境。由于多个P01项目对NOD鼠标的依赖 糖尿病模型和新型 STAT3 功能获得 (GOF) 小鼠系，大部分核心工作将 围绕这些线路的维护、分配和使用以及其他相关免疫 系统报告基因或免疫缺陷系。通过这种方式，核心可以帮助确保品质、遗传谱系、 以及共享 P01 研究人员合作研究所需的动物。