Animal Services

动物服务

• 批准号: 7645167
• 负责人: JEFFREY Victor RAVETCH
• 金额: $ 20.07万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7645167
• 关键词: Animal Husbandry; Animal Technicians; Animals; Antibiotic Therapy; Antigens; Autoimmune Process; Beds; CCL20 gene; Cell physiology; Dendritic Cells; Engineering; Food; Gene Deletion; Generations; Goals; Head; Housing; Immunocompromised Host; Maintenance; Mouse Strains; Pathway interactions; Program Research Project Grants; Purpose; Research Personnel; Role; Services; Standards of Weights and Measures; Sterilization for infection control; Stimulus; Time; Work; animal breeding; animal care; cost; immune function; in vivo; programs; prophylactic

A critical aspect of the program project proposal includes the in vivo analysis of dendritic cell function in specifically genetically engineered strains of mice. Two general types of mouse strains will be routinely utilized - those severely immunocompromised, the result of specific gene deletions in immune functions and autoimmune strains of mice that are hyper-responsive to a variety of antigen stimuli. The goals of the program project grant involve the manipulation of these animals to determine the role of innate DC subsets in maintaining tolerance and the pathways that are involved in that vital function. The generation and maintenance of these strains will be the function of the animal services core, headed by Dr. Jeffrey Ravetch. The purpose of this core will be to provide segregated facilities for the housing and breeding of the animal strains proposed in this program project. To support that function, the Director of LARC, Mr. Fred Quimby, has agreed to work with the program project investigators to implement the level of animal husbandry necessary. A full-time animal technician dedicated to this purpose will be required to provide the additional care these animals require, including bi-weekly bedding changes, prophylactic antibiotic treatment and the sterilization of all cages, bedding and food. This cost, along with the per diem established by the LARC for standard animal care, will cover the additional services required.

计划项目建议的一个关键方面包括对树突状细胞功能的体内分析 特定的基因工程菌株的小鼠菌株。通常将两种一般类型的鼠标菌株定期 利用 - 那些严重免疫功能低下的人，是免疫功能中特定基因缺失的结果 与多种抗原刺激相反的小鼠自身免疫性菌株。目标的目标 计划项目赠款涉及操纵这些动物以确定先天DC子集的作用 在维持耐受性和涉及该重要功能的途径方面。一代和 这些菌株的维护将是由Jeffrey Ravetch博士领导的动物服务核心的功能。 该核心的目的是为动物的住房和繁殖提供隔离的设施 该计划项目中提出的压力。为了支持该功能，LARC的主任Fred Quimby先生， 已经同意与计划项目调查人员合作实施畜牧业水平 必要的。一名专门用于此目的的全职动物技术人员将需要提供额外的 照顾这些动物需要，包括每两周的床上用品变化，预防性抗生素治疗和 所有笼子，床上用品和食物的灭菌。这笔费用以及LARC建立的Per Diem 标准动物护理将涵盖所需的其他服务。