Novel Function Investigation of Coagulation Factors in Defense System Regarding Infection: From Medaka (Oryzias latipes) to Human (Homo sapiens)

感染防御系统中凝血因子的新功能研究：从青鳉（Oryzias latipes）到人类（Homo sapiens）

• 批准号: 22KJ1527
• 负责人: MENG QI
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ1527/
• 关键词: メダカ; 血液凝固; 免疫; Extracellular traps; fibrinogen; anemia; infection; immunity

In my research, I use Japanese medaka, a popular model organism in research field, to study the relationship between the blood coagulation (blood clot formation) and immunity. Last year, I successfully established the fibrinogen gene-deficient medaka which cannot form normal blood clot. Meanwhile, I tried to induce the bacterial infection to medaka aim to study the immune system in medaka. During the infection study, I found in infected medaka blood sample, more string-like structures were observed, and these structures are considered as the extracellular traps (ETs) which is an innate immune response upon stimulant in human. The existence and mechanisms of ETs in medaka is still unclear. Therefore, investigation of medaka ETs is a new knowledge in medaka immune study and can also provide new viewpoint for related diseases’ treatment in human.

在我的研究中，我使用研究领域流行的模型生物日本青鳉来研究血液凝固（血凝块形成）与免疫之间的关系。去年，我成功地建立了纤维蛋白原基因缺陷的青鳉，它不能形成正常的血液。同时，我尝试诱导青鳉的细菌感染，目的是研究青鳉的免疫系统。在感染研究中，我发现在受感染的青鳉血液样本中，观察到了更多的线状结构，这些结构被认为是。细胞外陷阱(ETs)是人类对刺激物产生的先天性免疫反应。ETs在青鳉体内的存在及其机制尚不清楚，因此，对青鳉ETs的研究是青鳉免疫研究的一个新知识，也可以为青鳉免疫研究提供新的视角。人类相关疾病的治疗。

项目成果

Biochemical Analysis and and Investigation for the Novel Functions of Fibrinogen Using Gene-deficient Medaka (Oryzias latipes)

利用基因缺陷青鳉 (Oryzias latipes) 进行纤维蛋白原新功能的生化分析和研究

• 发表时间: 2022
• 作者: Qi MENG

フィブリンノーゲン欠損メダカの作製と表現型―貧血疾患モデルとしての解析

纤维蛋白原缺乏青鳉鱼的产生和表型 - 作为贫血疾病模型的分析

• 发表时间: 2022
• 作者: Qi MENG

Biochemical characterization of medaka (Oryzias latipes) fibrinogen gamma and its gene disruption resulting in anemia as a model fish

青鳉（Oryzias latipes）纤维蛋白原γ的生化特征及其基因破坏导致作为模式鱼的贫血

• DOI: 10.1093/jb/mvac065
• 发表时间: 2022
• 作者: Meng Qi;Watanabe Yuko;Tatsukawa Hideki;Hashimoto Hisashi;Hitomi Kiyotaka
• 通讯作者: Hitomi Kiyotaka

Biochemical Properties and Novel Function Investigation of the Blood Coagulation Factor, Fibrinogen, by Using Medaka (Oryzias latipes) as Model Organism

以青鳉（Oryzias latipes）为模式生物研究凝血因子纤维蛋白原的生化特性和新功能

• 发表时间: 2022
• 作者: MENG Qi