Modernization of a Shared-use Gnotobiotic Animal Core

共享使用知生动物核心的现代化

基本信息

批准号：
10532456
负责人：
JISUN PAIK
金额：
$ 7.19万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10532456
关键词：
Address Air Air Pressure Animal Behavior Animal Experimentation Animal Husbandry Animal Welfare Animals Area Avidity Breeding Budgets Canis familiaris Caring Communities Complex Core Facility Data Disease Disease Progression Electronic Mail Environment Environmental Monitoring Environmental Risk Factor Equipment Faculty Film Floods Food Foundations Funding Funding Agency Funding Opportunities Genetic Germ-Free Gnotobiotic Goals Grant Health Health Sciences Health care facility House mice Housing Human Human Resources Humidity In Vitro Individual Injections Institution Light Maintenance Medicine Methods Mission Modernization Monitor Mus Neoplasm Transplantation Noise Pilot Projects Privatization Process Public Health Publications Reproducibility Research Research Personnel Research Support Resources Role Schedule Side Specific qualifier value Sterility System Techniques Technology Telephone Temperature Time Time Management Training Translations Treatment Efficacy Universities Visual Washington Water air monitoring animal care animal facility comparative digital environmental change experimental study flexibility germ free condition high efficiency particulate air filter improved in vivo member microbiome microbiome research mouse model risk minimization user-friendly vibration welfare

项目摘要

PROJECT SUMMARY The Gnotobiotic Animal Core (GNAC) is a specialized mouse care facility that is shared by investigators within and outside of the University of Washington (UW). It is located in the specific pathogen free (SPF) vivarium in the Health Sciences Buildings at UW and occupies ~1750 sq ft consisting of four separate rooms. It is managed by two faculty members and highly trained GNAC Staff in the Department of Comparative Medicine. The GNAC is a complete research support facility: We create and care for gnotobiotic and germ-free mice housed in the core and provide technical support for diverse gnotobiotic research needs. A particular strength of the core is that new investigators can conduct small pilot projects by using the ready-to-go expertise of GNAC staff even if they do not have the extensive training needed for such studies. The GNAC has been operational for over seven years, and during this period, more than forty investigators from fifteen different research groups have used the GNAC. Over twenty publications have been generated with support from twenty-three federal grants and twelve private foundation/institutional grants. GNAC utilizes two housing systems: Flexible-film isolators are used for breeding and maintenance of various strains of GF mice, and isopositive cages are used for mice during experiments. The isopositive cages are highly specialized, air-tight, HEPA filtered, bioexclusion cages (ISOcage P caging manufactured by Tecniplast). When paired with using a biosafety cabinet and sterile technique, the isopositive cages offer a more user-friendly alternative to isolators for investigators to maintain and manipulate gnotobiotic mice. The goal of this application is to retrofit existing ISOcage P cages with a Digital Ventilated Cage Rack Environment Monitoring (DVC REM) system. The DVC REM system will monitor cage- and rack- level environments as well as global animal activity within each cage continuously. This modernization will greatly enhance several experimental and husbandry-related aspects of gnotobiotic research using mice: 1) improved experimental rigor, reproducibility and translation of experiments, resulting in reduced number of research animals needed to address complex research questions; 2) improved animal welfare by providing 24/7 monitoring that will alert investigators to problems with animal health or the caging environment; and 3) improved time management for GNAC staff during animal husbandry practices by streamlining scheduling of animal care- related tasks and preparing and rotating supplies. Successful completion of our proposed plan will broadly benefit all investigators using the facility by helping to maximize the use of limited resources and to improve reproducibility in animal research by providing unprecedented continuous monitoring of environment and animal activities.

项目摘要 Gnotobiotic Animal Core（GNAC）是一种专业的小鼠护理设施，由研究人员共享在华盛顿大学（UW）之外。它位于特定病原体无病原体（SPF）中 UW的健康科学大楼占据了约1750平方英尺，由四个独立的房间组成。它是管理的由两名教职员工和受过培训的GNAC员工在比较医学系中。 gnac 是一个完整的研究支持设施：我们创建和照顾和照顾gnotobiotic和无菌小鼠核心并为各种gnotobiotic研究需求提供技术支持。核心的特殊优势是新调查人员可以通过使用GNAC员工的现成专业知识来进行小型试点项目他们没有此类研究所需的广泛培训。 GNAC已经运作七个几年，在此期间，来自15个不同研究小组的四十多名调查人员使用了 gnac。在二十三个联邦赠款和十二份的支持下，已经生成了二十多个出版物私人基金会/机构赠款。 GNAC使用了两个住房系统：使用灵活的模具隔离器用于各种菌株的GF小鼠的繁殖和维护，以及等于阳性的笼子，用于小鼠实验。等速阳性笼子高度专业化，气密，HEPA过滤，生物排斥笼（等距由Tecniplast制造的P笼）。与使用生物安全柜和无菌技术配对时，等应阳性笼子为调查人员提供了一种更具用户友好的替代方案，以维护和操纵 gnotobiotic小鼠。该应用程序的目的是用数字通风改造现有的Isocage P笼笼环境监控（DVC REM）系统。 DVC REM系统将监视笼子和机架 - 水平环境以及每个笼子内的全球动物活动不断。这种现代化将极大地使用小鼠增强gnotobiotic研究的几个实验和饲养方面：1）改进实验严格，可重复性和实验的翻译，导致研究数量减少动物需要解决复杂的研究问题； 2）通过提供24/7来改善动物福利监视，这将提醒调查人员对动物健康或笼子环境的问题； 3）改进通过简化动物护理计划，在畜牧业习惯期间，GNAC员工的时间管理 - 相关任务以及准备和旋转供应。成功完成我们拟议的计划将广泛通过帮助最大化有限资源的使用并改善所有使用该设施的调查员动物研究中的可重复性通过提供空前的环境和动物的持续监测活动。