Functional genomics resources for the Drosophila - TR&D2

果蝇功能基因组学资源 - TR

基本信息

批准号：
10620329
负责人：
STEPHANIE E MOHR
金额：
$ 32.67万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10620329
关键词：
Address Aedes Animals Anopheles Genus Antibodies Arboviruses Area Bacteria Binding Binding Proteins Biological Assay Biology Boston CRISPR/Cas technology Cell Line Cells Chikungunya virus Clustered Regularly Interspaced Short Palindromic Repeats Collaborations Communicable Diseases Communities Culicidae Cultured Cells Dengue Virus Development Diptera Disease Disease Vectors Drosophila genus Emerging Communicable Diseases Fab Immunoglobulins Funding Genes Genome Genome engineering Human Biology Infection Infectious Diseases Research Insecta Insecticide Resistance Knock-out Knowledge Libraries Malaria Modeling Modification Mosquito Control Mutagens Nobel Prize Open Reading Frames Physiology Population Population Control Production Proteins Proteomics RNA interference screen Reagent Reporting Research Resources Roentgen Rays Site System Technology Testing Texas Tsetse Flies Universities Viral Vector Virus Diseases Visualization Wolbachia Yeasts Zika Virus experience experimental study fly functional genomics genetic analysis genetic technology genome annotation genome resource genome wide screen genome-wide human disease improved in vivo interest malaria transmission medical schools member mosquito-borne nanobodies pathogen pathogenic virus protein expression protein purification screening site-specific integration sterile insect technique technology development tool vector vector mosquito

项目摘要

PROJECT SUMMARY – TR&D2 Drosophila serves as a model not only of human biology but also of the biology of other dipterans, including mosquito vectors of disease, and provides an instructive example of technologies that can be applied to direct study of other insects. In this project, we focus on development of functional genomics and proteomics resources for the study of mosquito vectors of disease, with a particular focus on development of CRISPR pooled screens for Aedes mosquitoes, which are vectors of viral pathogens including Zika, Dengue, and Chikungunya viruses, and for Anopheles mosquitoes, the vector for malaria. We will additionally develop a pipeline for isolation of robust protein binders, i.e. nanobodies, for use in visualization and in vivo functional blocking, based on a yeast nanobody display platform. A set of collaborators with established assays in mosquitoes will help us to test and improve the screening and nanobody technologies. Altogether, this project will provide much-needed reagents, including a platform for genome-wide screening, for direct study of pathogen entry and other aspects of mosquito biology relevant to infectious diseases and mosquito population control.

项目摘要 - TR＆D2 果蝇不仅是人类生物学的模型，而且是其他双翅目生物学的模型，包括疾病的蚊子载体，并提供了一个可以应用于直接的技术的启发性示例研究其他昆虫。在这个项目中，我们专注于功能基因组学和蛋白质组学的发展研究蚊子媒介的资源，特别着眼于CRISPR的发展埃德斯蚊子的合并屏幕，它们是病毒病原体的媒介，包括Zika，登革热和 chikungunya病毒，以及疟疾载体的蚊子。我们还将开发一个用于分离可视化和体内功能的稳健蛋白质粘合剂的管道，即纳米生物。基于酵母纳米型显示平台的阻塞。一组合作者，有既定测定法蚊子将帮助我们测试和改善筛查和纳米机构技术。总共，这个项目将提供急需的试剂，包括用于全基因组筛查的平台，用于直接研究病原体的进入以及与传染病和蚊子种群有关的蚊子生物学的其他方面控制。