Shared Resource 05: Collaborative Functional Genomics

共享资源 05：协作功能基因组学

基本信息

批准号：
10062890
负责人：
STEPHANIE E MOHR
金额：
$ 14.97万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-03-10 至 2021-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10062890
关键词：
Automation Bioinformatics Biological Biological Assay CRISPR/Cas technology Cancer Center Categories Cells Cellular biology Chemistry Clustered Regularly Interspaced Short Palindromic Repeats Code Communities Consultations Dana-Farber Cancer Institute Data Data Analyses Development Doctor of Philosophy Drosophila genus Education Educational workshop Equipment Fluorescence Microscopy Funding Gene set enrichment analysis Generations Genes Genetic Genome engineering Genomic approach Genomics Shared Resource Group Meetings Guide RNA Home Human Image Imaging Device Institutes Institution Knock-out Laser Scanning Cytometry Libraries Liquid substance Luciferases Malignant Neoplasms MicroRNAs Microscopy Mission Mus Open Reading Frames Output Pathway interactions Positioning Attribute Production Proteins RNA Interference RNA interference screen Reagent Reporter Research Personnel Resource Sharing Resources Running Services Site Small Interfering RNA Technology Training Training and Education Triage Untranslated RNA Validation Visualization assay development base cancer prevention cancer therapy data quality design experience functional genomics gain of function genetic approach genome-wide high throughput screening improved in vivo instrument loss of function medical schools meetings member overexpression prospective protein complex screening service member small hairpin RNA tumor progression

项目摘要

Collaborative Functional Genomics Shared Resource Project Summary / Abstract Understanding the basic biological mechanisms that underlie the development and progression of cancer is fundamental to finding better approaches to cancer prevention and treatment. Loss-of-function genetic approaches, such as high-throughput RNA interference (RNAi) and CRISPR/Cas9, as well as gain-of-function approaches such as open reading frame (ORF) over-expression and CRISPR-activation (CRISPR-a) provide cutting-edge strategies to identify new cancer-related genes and pathways. The Collaborative Functional Genomics Core offers a single portal through which DF/HCC members gain access to technologies, reagents, services, and scientific consultation. The Core mission is to support the widest possible range of functional genomics screening projects; rapidly disseminate information about new functional genomics approaches; and help researchers navigate from concept and assay development to high-throughput screening, analysis, and validation.

协作功能基因组学共享资源项目概要/摘要了解癌症发生和进展的基本生物学机制是寻找更好的癌症预防和治疗方法的基础。遗传性功能丧失方法，例如高通量 RNA 干扰 (RNAi) 和 CRISPR/Cas9，以及功能获得开放阅读框 (ORF) 过表达和 CRISPR 激活 (CRISPR-a) 等方法提供识别新的癌症相关基因和途径的尖端策略。协作功能 Genomics Core 提供了一个单一门户，DF/HCC 成员可以通过该门户获取技术、试剂、服务、科学咨询。核心使命是支持尽可能广泛的功能基因组学筛选项目；快速传播有关新功能基因组学方法的信息；和帮助研究人员从概念和分析开发转向高通量筛选、分析和验证。