ShEEP Request for the purchase of a research- grade Cell Imaging Multi-mode Reader

ShEEP 请求购买研究级细胞成像多模式读取器

• 批准号: 10739194
• 负责人: PARAMITA M. GHOSH
• 金额: --
• 依托单位: VA NORTHERN CALIFORNIA HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10739194
• 关键词: Address; Affect; Ambulatory Care Facilities; Apoptosis; Automation; Basic Science; Biochemical; Bioinformatics; Biological Assay; Calcium; California; Cancer Research Project; Cell Count; Cell Cycle; Cell Survival; Cells; Clinical Research; Collaborations; Colon Carcinoma; Color; Country; DNA Damage; Data; Detection; Development; Disease; Drug Interactions; Elderly; Ensure; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Funding; Gases; Genetic; Genomics; Glycolysis; Goals; Grant; Healthcare; Healthcare Systems; Image; Immunofluorescence Immunologic; Immunohistochemistry; Immunologics; Invaded; Kinetics; Knowledge; Label; Laboratory Research; Life; Luciferases; Lymphoma; Malignant Neoplasms; Malignant neoplasm of lung; Malignant neoplasm of prostate; Measures; Medical center; Metabolism; Microscopy; Molecular; Names; Necrosis; Pathologic; Pathway interactions; Patients; Phagocytosis; Phase; Phenotype; Physiological; Principal Investigator; Process; Proteins; Reader; Reagent; Reporter; Reproducibility; Reproducibility of Results; Research; Research Personnel; Research Project Grants; Rural; Scanning; Scientist; Secure; Seminal; Services; Sheep; Slide; Standardization; Steroids; Sulfatases; System; Temperature; Testing; Therapeutic; Time; Tissues; Toxic effect; United States National Institutes of Health; Veterans; advanced prostate cancer; anticancer research; castration resistant prostate cancer; cell motility; cellular imaging; chemotherapy; colon cancer patients; cytate; digital; functional genomics; genotoxicity; improved; innovation; instrument; laboratory development; live cell imaging; luminescence; metabolomics; novel; novel therapeutic intervention; novel therapeutics; peptide drug; pharmacologic; pre-clinical research; programs; protein protein interaction; small molecule; small molecule inhibitor; stem cell differentiation; therapeutic target; therapy resistant; three dimensional cell culture; tool; tumor metabolism; tumor progression; tumorigenesis; user-friendly; Address; Affect; Ambulatory Care Facilities; Apoptosis; Automation; Basic Science; Biochemical; Bioinformatics; Biological Assay; Calcium; California; Cancer Research Project; Cell Count; Cell Cycle; Cell Survival; Cells; Clinical Research; Collaborations; Colon Carcinoma; Color; Country; DNA Damage; Data; Detection; Development; Disease; Drug Interactions; Elderly; Ensure; Enzyme-Linked Immunosorbent Assay; Equipment; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Funding; Gases; Genetic; Genomics; Glycolysis; Goals; Grant; Healthcare; Healthcare Systems; Image; Immunofluorescence Immunologic; Immunohistochemistry; Immunologics; Invaded; Kinetics; Knowledge; Label; Laboratory Research; Life; Luciferases; Lymphoma; Malignant Neoplasms; Malignant neoplasm of lung; Malignant neoplasm of prostate; Measures; Medical center; Metabolism; Microscopy; Molecular; Names; Necrosis; Pathologic; Pathway interactions; Patients; Phagocytosis; Phase; Phenotype; Physiological; Principal Investigator; Process; Proteins; Reader; Reagent; Reporter; Reproducibility; Reproducibility of Results; Research; Research Personnel; Research Project Grants; Rural; Scanning; Scientist; Secure; Seminal; Services; Sheep; Slide; Standardization; Steroids; Sulfatases; System; Temperature; Testing; Therapeutic; Time; Tissues; Toxic effect; United States National Institutes of Health; Veterans; advanced prostate cancer; anticancer research; castration resistant prostate cancer; cell motility; cellular imaging; chemotherapy; colon cancer patients; cytate; digital; functional genomics; genotoxicity; improved; innovation; instrument; laboratory development; live cell imaging; luminescence; metabolomics; novel; novel therapeutic intervention; novel therapeutics; peptide drug; pharmacologic; pre-clinical research; programs; protein protein interaction; small molecule; small molecule inhibitor; stem cell differentiation; therapeutic target; therapy resistant; three dimensional cell culture; tool; tumor metabolism; tumor progression; tumorigenesis; user-friendly

PROJECT SUMMARY VA Northern California Health Care System (VANCHCS) serves over 500,000 Veterans and is one of the largest catchments in the country, including a large rural component. VANCHCS operates research projects at both the Sacramento VA Medical Center (Mather) and the VA Martinez Outpatient Clinic. We conduct research to discover knowledge, develop VA scientists and health care leaders, and create innovations that advance health care for Veterans and the nation. Our Biomedical Laboratory Research & Development Service conducts preclinical and clinical research to understand life processes from the molecular, genomic, and physiological levels regarding diseases affecting Veterans, with a major emphasis on cancer. Some key objectives of our research program are to identify new cancer therapeutic targets and better understand the molecular mechanisms and pathways underlying cancer progression, such as therapeutics resistance. To accomplish these goals, we employ functional genomics, metabolomics, bioinformatics, genetics, and pharmacological approaches to determine the physiological and pathological functions and action mechanisms of key regulators to elucidate the mechanisms of action of the new therapeutics. To help our research program reach these goals, which will directly benefit our Veterans, we are requesting funds for a Cell Imaging Multi-mode Reader. This system would allow us to greatly expand the breadth of our research capabilities at VANCHCS. For example, our research program would be able to perform brightfield and immunofluorescence live cell imaging on both 2D and 3D cell cultures, as well as a multitude of biochemical assays, such as cell viability and toxicity, cell migration and invasion, apoptosis and necrosis, DNA damage, phagocytosis, phenotypic assays, and metabolomic assays, to name a few. This single piece of equipment would strongly enhance our current Cancer Research program and increase our ability to apply for competitive grant funding. Currently, our Cancer Research group has several VA- and NIH-funded studies that would benefit directly from this Cell Imaging Multi-mode Reader; 1) function and mechanism of small molecules in castration resistant prostate cancer; 2) periclinal mechanistic and immunologic studies for a novel lymphoma treatment; 3) therapeutic targeting steroid sulfatase and /or Wnt5A for advanced prostate cancer; 4) targeting tumor metabolism in prostate cancer; 5) develop potent and deliverable therapeutic peptides and small molecule inhibitors as a novel therapeutic for Veteran colorectal cancer patients. Ultimately, our goal is to identify cancer relevant therapeutic targets and develop novel therapeutics to better treat Veterans.

项目摘要 弗吉尼亚州北加州医疗保健系统（VANCHCS）为500,000多名退伍军人提供服务，是 该国最大的集水区，包括大型农村组成部分。 Vanchcs运行 萨克拉曼多VA医疗中心（Mather）和VA Martinez的研究项目 门诊诊所。我们进行研究以发现知识，发展VA科学家和医疗保健 领导者，并创造创新，以推动退伍军人和国家的医疗保健。我们的生物医学 实验室研发服务进行临床前和临床研究以了解 关于影响疾病的分子，基因组和生理水平的生命过程 退伍军人，重点是癌症。我们研究计划的一些关键目标是 确定新的癌症治疗靶标，并更好地了解分子机制和 癌症进展的途径，例如治疗性耐药性。完成这些 目标，我们采用功能性基因组学，代谢组学，生物信息学，遗传学和药理学 确定关键的生理和病理功能和动作机制的方法 监管机构阐明新治疗剂的作用机制。帮助我们的研究 计划达到这些目标，这将直接使我们的退伍军人受益，我们正在为牢房提供资金 成像多模式读取器。该系统将使我们大大扩大我们的广度 Vanchcs的研究能力。例如，我们的研究计划能够执行 在2D和3D细胞培养物上的Brightfield和免疫荧光活细胞成像，以及 多种生化测定，例如细胞活力和毒性，细胞迁移和侵袭， 细胞凋亡和坏死，DNA损伤，吞噬作用，表型测定和代谢组学测定， 命名几个。这件设备将大大增强我们当前的癌症研究 计划并提高我们申请竞争性赠款资金的能力。目前，我们的癌症 研究小组有一些VA和NIH资助的研究，可以直接从该细胞中受益 成像多模式读取器； 1）小分子在抑制性抗性中的功能和机制 前列腺癌； 2）新型淋巴瘤治疗的周期机械和免疫学研究； 3）针对晚期前列腺癌的类固醇硫酸酶和 /或Wnt5a的治疗性靶向； 4）目标 前列腺癌中的肿瘤代谢； 5）开发有效且可交付的治疗性肽和 小分子抑制剂作为老将结直肠癌患者的新型治疗方法。最终，我们的 目标是确定癌症相关的治疗靶点并开发新型治疗剂以更好地治疗 退伍军人。