Functional genomics resources for the Drosophila - TR&D2

果蝇功能基因组学资源 - TR

基本信息

批准号：
10436793
负责人：
STEPHANIE E MOHR
金额：
$ 32.67万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-01 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10436793
关键词：
Address Aedes Animals Anopheles Genus Antibodies Arboviruses Area Bacteria Binding Proteins Biological Assay Biology Boston CRISPR/Cas technology Cell Line Cells Chikungunya virus Clustered Regularly Interspaced Short Palindromic Repeats Communicable Diseases Communities Culicidae Cultured Cells Dengue Virus Development Diptera Disease Disease Vectors Drosophila genus Emerging Communicable Diseases Fab Immunoglobulins Funding Genes Genome Genome engineering Human Biology Infection Infectious Diseases Research Insecta Insecticide Resistance Instruction Knock-out Knowledge Lead Libraries Malaria Modeling Modification Mosquito Control Mutagens Open Reading Frames Physiology Population Population Control Production Proteins Proteomics RNA interference screen Reagent Reporting Research Resources Roentgen Rays Site System Technology Testing Texas Tsetse Flies Universities Viral Vector Virus Diseases Visualization Wolbachia Yeasts Zika Virus base experience experimental study fly functional genomics genetic analysis genetic technology genome annotation genome wide screen genome-wide human disease improved in vivo innovation interest medical schools member mosquito-borne nanobodies pathogen pathogenic virus protein expression protein purification screening site-specific integration sterile insect technique technology development tool vector vector mosquito

项目摘要

PROJECT SUMMARY – TR&D2 Drosophila serves as a model not only of human biology but also of the biology of other dipterans, including mosquito vectors of disease, and provides an instructive example of technologies that can be applied to direct study of other insects. In this project, we focus on development of functional genomics and proteomics resources for the study of mosquito vectors of disease, with a particular focus on development of CRISPR pooled screens for Aedes mosquitoes, which are vectors of viral pathogens including Zika, Dengue, and Chikungunya viruses, and for Anopheles mosquitoes, the vector for malaria. We will additionally develop a pipeline for isolation of robust protein binders, i.e. nanobodies, for use in visualization and in vivo functional blocking, based on a yeast nanobody display platform. A set of collaborators with established assays in mosquitoes will help us to test and improve the screening and nanobody technologies. Altogether, this project will provide much-needed reagents, including a platform for genome-wide screening, for direct study of pathogen entry and other aspects of mosquito biology relevant to infectious diseases and mosquito population control.

项目摘要 – TR&D2 果蝇不仅是人类生物学的模型，也是其他双翅目动物的生物学模型，包括蚊子传播疾病，并提供了可用于直接控制疾病的技术的指导性示例在这个项目中，我们重点关注功能基因组学和蛋白质组学的发展。用于疾病蚊媒研究的资源，特别关注 CRISPR 的开发伊蚊的汇总筛查，伊蚊是病毒病原体的载体，包括寨卡病毒、登革热病毒和基孔肯雅病毒和按蚊（疟疾的传播媒介）我们还将开发一种病毒。用于分离强大的蛋白质结合剂（即纳米抗体）的管道，用于可视化和体内功能阻断，基于酵母纳米抗体展示平台，一组合作者已建立了检测方法。蚊子将帮助我们测试和改进这个项目的筛选和纳米抗体技术。将提供急需的试剂，包括全基因组筛选平台，用于直接研究病原体进入以及与传染病和蚊子种群相关的蚊子生物学的其他方面控制。