Investigating the Role of Gr23 Gene in the Mating Capacity of Malaria Vector Mosquito Anopheles coluzzii

Gr23基因在疟疾媒介蚊子按蚊交配能力中的作用研究

• 批准号: 10425425
• 负责人: LAURENCE J ZWIEBEL
• 金额: $ 7.93万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-06-09 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10425425
• 关键词: Address; Adopted; Aedes; Afferent Neurons; Alleles; Animals; Anopheles Genus; Arthropods; Behavior; Blood; Breeding; Candidate Disease Gene; Carbon Dioxide; Cessation of life; Chemicals; Code; Complex; Cues; Culicidae; Defect; Dengue; Detection; Diptera; Environment; Exhibits; Failure; Female; Filarial Elephantiases; Gene Targeting; Genes; Human; Hydrocarbons; In Vitro; Japanese Encephalitis; Leg; Malaria; Maxilla; Mediating; Membrane; Molecular; Molecular Target; Mosquito Control; Neurons; Odorant Receptors; Odors; Parasites; Partner in relationship; Pattern; Persons; Pheromone; Plasmodium falciparum; Play; Process; Public Health; Reproduction; Research Proposals; Role; Semiochemicals; System; Testing; Triad Acrylic Resin; West Nile Encephalitis; Work; Xenopus oocyte; Yellow Fever; Zika Virus; appendage; base; carbon dioxide receptor; feeding; in vivo; labia; male; mating behavior; mutant; novel; olfactory sensory neurons; pathogen; receptor; reproductive; response; transcriptomics; vector; vector mosquito

Project Summary As one of the most important vectors for the malaria parasite Plasmodium falciparum, which infected approximately 228 million people worldwide in 2018 and accounted for more than 400,000 deaths (WHO, 2019), the An. coluzzii mosquito has long been a formidable concern to public health. These mosquitoes rely heavily on their chemosensory system to detect chemical cues involved in the mating process, including aggregation pheromones and cuticular hydrocarbons. Swarm aggregation pheromones increase the possibility of encountering female mosquitoes while cuticular hydrocarbons help male mosquitoes recognize conspecific females. A recent finding of ours indicated that not only were Gr23-null mutants insensitive to carbon dioxide stimulation, they also appeared to have some mating defect, as they failed to self-breed. Previous transcriptomic studies in Aedes mosquitoes indicated that Gr23 is expressed in CO2-sensitive neurons in the maxillary palps, but presents the highest expression among all gustatory receptors in the antennae and other chemosensory appendages, which suggests additional roles of Gr23 in chemosensation beyond CO2 reception. Therefore, the aim of this research proposal is to determine the role of Gr23 in the mating capacity of An. coluzzii. Here, we hypothesize that the observed self-breeding defect of Gr23 mutants is due to their inability to detect mating-related chemical cues, and that the Gr23 receptor plays a critical role in this chemosensory process. Accordingly, the fundamental questions I propose to address are: (1) what is the expression pattern of Gr23 in the chemosensory neurons of the mating-related appendages in An. coluzzii? (2) What is the molecular function of Gr23 in detecting the mating-related semiochemicals? In addressing these questions, this work will further our understanding of the role of Gr23 in the mosquito's odor-mediated mating process, which would have great potential as a novel molecular target in mosquito control.

项目摘要 作为疟原虫疟原虫恶性疟原虫的最重要的载体之一，它感染了 2018年全球约有2.28亿人口，死亡人数超过40万人（谁， 2019），长期以来，Coluzzii蚊子一直是公共卫生的强烈关注。这些蚊子依靠 大量的化学感应系统，以检测参与交配过程的化学提示，包括 聚集信息素和表皮烃。群汇聚信息素增加了可能性 遇到雌性蚊子的同时，表皮碳氢化合物有助于雄性蚊子识别同种 女性。我们最近的发现表明，不仅GR23-NULL突变体对二氧化碳不敏感 刺激性，由于他们未能自我批次，它们似乎也有一些交配缺陷。以前的 艾德斯蚊子中的转录组研究表明，GR23在二氧化碳敏感神经元中表达 上颌骨，但在触角和其他触角中的所有味觉受体中表现最高 化学感应附属物，这表明GR23在CO2以外的化学通敏中的其他作用 接待。因此，该研究建议的目的是确定GR23在交配能力中的作用 一个coluzzii。在这里，我们假设观察到的GR23突变体的自我繁殖缺陷是由于它们的 无法检测与交配相关的化学提示，并且GR23受体在此起着至关重要的作用 化学感应过程。因此，我提出的基本问题是：（1）什么是 An中与交配相关附件的化学感应神经元中GR23的表达模式。 coluzzii？ （2） GR23检测与交配相关的半化学物质的分子功能是什么？在解决这些问题时 问题，这项工作将进一步理解GR23在蚊子的气味介导的交配中的作用 在蚊子控制中，这将具有巨大的分子靶标的过程。