Characterization of Anopheline Odorant Receptors

按蚊气味受体的表征

基本信息

批准号：
9552301
负责人：
LAURENCE J ZWIEBEL
金额：
$ 56.92万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-06 至 2020-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9552301
关键词：
Acetophenones Acids Address Adult Affect African Anopheles Genus Anopheles gambiae Behavior Behavioral Biological Blood Chemicals Code Collaborations Complex Cues Culicidae Data Data Set Dengue Development Disease Electrophysiology (science)Elements Exhibits Family Female Generations Heart Human Infection Insect Vectors Insecta International Investigation Kairomones Location Malaria Maps Mediating Modeling Molecular Mosquito Control Neurons Odorant Receptors Odors Olfactory Pathways Olfactory Receptor Neurons Oviposition Physiological Physiology Plasmodium falciparum Play Process Protocols documentation Role Salivary Glands Signal Pathway Signal Transduction Site Smell Perception Specificity Sporozoites System West Nile Encephalitis Yellow Fever Zika Virus appendage base behavioral response combinatorial comparative design disease transmission feeding human disease in vivo insight malaria infection novel novel strategies pathogen preference programs protein expression protein function receptor response sensory input transcriptome transcriptome sequencing transmission process vector vector mosquito vector transmission

项目摘要

Project Summary/Abstract Chemosensory responses are critical components in the control of several essential behaviors of insects that are vectors for pathogens responsible for many important human diseases. In particular, olfaction plays a major role in host seeking and oviposition preference/selection behaviors of blood-feeding female mosquitoes and, as such, constitutes a critical component of the mosquito's ability to transmit diseases such as malaria, dengue, yellow fever, Zika and West Nile virus encephalitis. Within this context, and together with our colleagues, we have undertaken a molecular, cellular and organismal examination of several elements of the olfactory signal transduction cascade in the principal African malaria vector mosquitoes Anopheles gambiae, An. coluzzii, An. arabiensis and An. funestus. An increased understanding of olfactory mechanisms and their underlying chemical cues provides insight into the processes of insect behavioral responses in general and disease transmission in particular and would likely be instrumental in the development of novel mosquito control strategies. In this context we have made significant progress in the characterization of several aspects of olfactory process in mosquito systems leading to, among other things, the identification and functional characterization of a family of odorant receptor proteins that lie at the heart of the olfactory signaling pathway. Building on those advances, this proposal for competitive renewal focuses on extending several elements of our ongoing program including characterization of: (1) the in vivo impact of biologically relevant Plasmodium falciparum infections on OR expression and OR-based olfactory receptivity, (2) the olfactory physiology and functional role of labellum ORs in An. coluzzii and (3) an examination of anthropophily via a comparative analysis of OR expression and functionality across Anophelines.

项目摘要/摘要化学感应反应是控制几种基本行为的关键组成部分昆虫是导致许多重要人类疾病的病原体的媒介。特别是嗅觉在宿主寻求和排卵偏好/喂食女性的选择行为中起着重要作用因此，蚊子等构成了蚊子传播这种疾病能力的关键组成作为疟疾，登革热，黄热病，寨卡病毒和西尼罗河病毒脑炎。在这种情况下，以及我们的同事，我们对几个元素的分子，细胞和有机检查进行了主要非洲疟疾媒介蚊子的嗅觉信号转导级联冈比亚，一个。 coluzzii，an。阿拉伯语和Funestus。对嗅觉机制的了解增加了它们的基本化学提示提供了对昆虫行为反应过程的见解尤其是一般和疾病的传播，可能会在新的发展中发挥作用蚊子控制策略。在这种情况下，我们在表征几个方面取得了重大进展蚊子系统中嗅觉过程的各个方面，导致识别和嗅觉信号中心的气味受体蛋白家族的功能表征路径。在这些进步的基础上，这项竞争性更新的提议着重于扩展几个我们正在进行的计划的要素包括：（1）生物学相关的体内影响恶性疟原虫感染或表达和 /或基于嗅觉的接受性，（2）嗅觉 Labellum ors在An中的生理和功能作用。 Coluzzii和（3）通过A检查拟人化的对围绕植物的表达或功能的比较分析。