CSHL Advanced Techniques in Molecular Neuroscience Course

CSHL 分子神经科学高级技术课程

• 批准号: 10578702
• 负责人: DAVID J. STEWART
• 金额: $ 12.86万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10578702
• 关键词: ATAC-seq; Applied Research; Area; Attention; Bacterial Artificial Chromosomes; Basic Science; Binding; Biological Assay; Cell Culture Techniques; Chromatin; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Course Content; Detection; Disease; Educational process of instructing; Electrophysiology (science); Engineering; Ensure; Environment; Exercise; Faculty; Female; Funding; Future; Home; Image; Immunoprecipitation; Individual; Institution; Laboratories; Mainstreaming; Mental Health; Messenger RNA; Methodology; Methods; Molecular; Molecular Neurobiology; Neurobiology; Neurosciences; Neurosciences Research; New York; Participant; Pharmacology; Physicians; Postdoctoral Fellow; Preparation; Process; Publications; RNA; RNA Binding; Reaction; Reporting; Research; Research Personnel; Role; Running; Scientist; Series; Specific qualifier value; Students; Techniques; Time; Tissues; Training; Training Programs; Transgenic Organisms; Translating; Underrepresented Minority; United States; Update; Viral Vector; Work; astrocyte progenitor; design; experience; forging; gene delivery system; genome editing; genome-wide; graduate student; in vivo; instructor; interest; laboratory curriculum; laboratory manuals; lecturer; medical specialties; member; mid-career faculty; nerve stem cell; nervous system disorder; neural; online resource; programs; protein crosslink; recruit; tool; transcription factor; vector

ABSTRACT CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2020– 2024, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive sixteen day hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include quantitative assays of RNAs bound to protein by crosslinking immunoprecipitation (CLIP), translating mRNA isolation from specified neural subtypes (TRAP), tissue clearing methodologies combined with single RNA molecule detection by hybridization chain reaction (HCR), preparation of pure astrocyte and neural progenitor cell cultures, design and use of engineered viral vectors in neuroscience research, design and use of CRISPR/Cas genome editing tools, practical exercises in gene delivery systems in vivo, genome-wide assessment of chromatin state and transcription factor binding by ATACseq and Cut & Run, and use and design of BAC transgenic vectors. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

抽象的 CSHL 分子神经科学先进技术实验室课程 拟议的冷泉港实验室 (CSHL) 课程“分子高级技术” 神经科学 (ATMN) 将于 2020 年 7 月至 2024 年每年举办一次，是 CSHL 研究生课程的一部分 ATMN 神经生物学培训计划是一个为期 16 天的短期强化实践课程。 帮助学生做好直接进入利用先进和/或专业技术的研究的准备 神经生物学研究实验模块当代方面的分子技术。 该课程将包括通过交联与蛋白质结合的 RNA 的定量分析 免疫沉淀 (CLIP)、翻译从特定神经亚型 (TRAP)、组织中分离的 mRNA 清除方法与杂交链式反应单RNA分子检测相结合 （HCR），纯星形胶质细胞和神经祖细胞培养物的制备，工程化的设计和使用 病毒载体在神经科学研究中，CRISPR/Cas基因组编辑工具的设计和使用，实用 体内基因传递系统的练习、染色质状态的全基因组评估和 ATACseq 和 Cut & Run 的转录因子结合，以及 BAC 转基因的使用和设计 向量的实验技术是在更广泛的概念进步的背景下教授的。 他们可能会提供该课程邀请在其领域做出重大贡献的讲师。 提供有关当前研究的最新报告。受训者由课程教师选出。 申请者群体更大，范围从研究生到高级调查员和研究人员 由于本课程持续时间短，高级和初级医师都可以参加。 并在远离其他要求的环境中接受短期、高强度的培训 ATMN 课程还为知名科学家提供了一个不寻常的机会 将这些先进技术应用于自己的研究兴趣，或在重要专业中进行再培训 他们可能不熟悉课程中教授的方法和概念。 通过出版实验室手册向更广泛的研究界传播 在线资源。