CSHL Single Cell Analysis Course (2023-2027)

CSHL单细胞分析课程（2023-2027）

• 批准号: 10627446
• 负责人: DAVID J. STEWART
• 金额: $ 16.16万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-24 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627446
• 关键词: Applied Research; Area; Attention; Basic Science; Bioinformatics; Biomedical Research; Cell Separation; Cells; Cellular biology; Child Health; Collaborations; Communities; Course Content; DNA analysis; Data; Data Analytics; Disease; Educational process of instructing; Electrophysiology (science); Ensure; Environment; Faculty; Familiarity; Funding; Future; Genomic DNA; Goals; Home; Human Development; Image; Individual; Institution; Laboratories; Libraries; Mainstreaming; Mass Spectrum Analysis; Methodology; Methods; Molecular; Molecular Analysis; Molecular Biology; Neurosciences; New York; Participant; Pathologic; Pharmacology; Physicians; Postdoctoral Fellow; Preparation; Process; Proteomics; Publications; Reporting; Research; Research Personnel; Role; Scientist; Series; Students; Techniques; Time; Tissues; Training; Training Programs; United States; Update; Western Blotting; Work; X-Ray Tomography; biological research; course module; experience; forging; graduate student; instructor; interest; laboratory curriculum; laboratory manuals; lecturer; medical specialties; member; metabolomics; mid-career faculty; online resource; programs; single cell analysis; single cell sequencing; single molecule; single-cell RNA sequencing; transcriptome sequencing

ABSTRACT CSHL Laboratory Course in Single Cell Analysis The proposed Cold Spring Harbor Laboratory (CSHL) course Single Cell Analysis to be held annually in July 2023– 2027, is part of the CSHL’s postgraduate training program in cell and molecular biology. This is a short, intensive 2 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of singe cell-based research. General themes to be covered during the course include quantitative single cell analysis by RNAseq, genomic DNA analysis, proteomics, and metabolomics. Specific experimental techniques taught in the course include: 1) Droplet- and microwell based single cell isolation; 2) Single cell RNA seq library preparation; 3) Single molecule FISH; 4) Single cell peptidomics; 5) Single cell western blot; 6) Photoactivatable single cell probes; 7) Single cell mass spectrometry; 8) Soft X-ray tomography; 9) Introductory single cell sequencing analysis. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

抽象的 单细胞分析中的CSHL实验室课程 拟议的冷春港实验室（CSHL）课程单细胞分析将每年进行 2023 - 2027年7月，是CSHL细胞和分子生物学研究生培训计划的一部分。 这是一门简短的2周动手实践课程，使学生直接进入 利用当代的高级和/或专业分子技术的研究 基于单元的研究的各个方面。课程期间要涵盖的一般主题包括 通过RNASEQ，基因组DNA分析，保护剂和代谢组学的定量单细胞分析。 课程中教授的特定实验技术包括：1）基于液滴和微孔的单个 细胞分离； 2）单细胞RNA SEQ库制备； 3）单分子鱼； 4）单细胞 肽组学； 5）单细胞蛋白质印迹； 6）可光活化的单细胞探针； 7）单细胞质量 光谱法； 8）软X射线断层扫描； 9）入门单细胞测序分析。实验 在它们可能提供的更广泛的概念进步的背景下，教授技术。 课程邀请的讲师在其领域做出了重大贡献以付出最新 有关当前研究的报告。学员是由较大池的课程教师选择的 申请人和从研究生到高级研究人员和研究医生的范围。因为 在本课程的短时间内，高年级以及初中的人可以参加并获得一小段时间 在远离其他时间和关注的其他需求的环境中，进行了激烈的培训。 该课程还为成熟的科学家提供了不寻常的机会应用这些高级 具有自己的研究兴趣的技术，或在神经科学中的重要专业中重新训练 他们可能不熟悉的。课程中教授的方法和概念被传播到 通过出版实验室手册和在线资源的更广泛的研究社区。