Defining the conformational control of nitric oxide synthases by a multipronged approach

通过多管齐下的方法定义一氧化氮合酶的构象控制

• 批准号: 10571224
• 负责人: Changjian Feng
• 金额: $ 8.91万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10571224
• 关键词: Administrative Supplement; Alzheimer' s Disease; Award; Bioinorganic Chemistry; Computer software; Development; Disease; Environment; Equipment; Family; Financial Support; Fourier Transform; Funding; Institution; Investigation; Investments; Methodology; Methods; Molecular Conformation; New Mexico; Nitric Oxide Synthase; Parents; Performance; Pharmacy facility; Protein Dynamics; Proteins; Research; Research Personnel; Resolution; Sampling; Site; Spectroscopy, Fourier Transform Infrared; Speed; Stroke; System; Techniques; United States National Institutes of Health; Universities; biophysical chemistry; college; design; effective therapy; infrared spectroscopy; instrument; instrumentation; novel therapeutics; parent project; protein complex

PROJECT SUMMARY The parent award is on deciphering conformational dynamics of nitric oxide synthase proteins through an integrated approach using advanced spectroscopic techniques. Fourier transform infrared (FT IR) spectroscopy is essential to the proposed studies of short-range conformational sampling (Aims 2 and 3) in the parent project. Here, we request funds to purchase a state-of-the-art Bruker VERTEX 80 FT IR spectrometer to enhance our IR research capabilities. Combining high sensitivity, resolution and speed with enhanced expandability and easy-to-use FT IR software, the VERTEX 80 system is the answer for demanding protein IR research. The high-performance research-grade instrument is designed to be top of the line. The superior capabilities of the system will be necessary for our efforts to develop and optimize our workflow and methods for site-specific IR spectroscopic investigation of local protein dynamics. Our findings will greatly benefit other investigators using IR methodologies for large, complex proteins. Therefore, the requested equipment will help the parent project to exert a sustained, powerful influence on the research fields of biophysics and bioinorganic chemistry. Moreover, the administrative supplement will enhance the scientific environment for NIH-funded investigators at the University of New Mexico, both within and outside of the College of Pharmacy. To our knowledge, no comparable instrumentation exists in educational institutions within the region. Dean Godwin has committed substantial financial support to this supplement request since this is a great investment for the College. Taken as a whole, the proposed supplement will increase the parent award’s overall impact within the original scope and bolster research quality and depth at our institution.

项目摘要 父母奖是关于一氧化氮合酶蛋白的解密构象动力学的 使用高级光谱技术的集成方法。傅立叶变换红外（ft ir） 光谱对于提出的短期构象采样（目标2和3）的研究至关重要。 父项目。在这里，我们要求资金购买最先进的布鲁克顶点80英尺红外光谱仪 增强我们的IR研究能力。将高灵敏度，分辨率和速度与增强相结合 顶点80系统的扩展性和易于使用的FT IR软件是要求蛋白质IR的答案 研究。高性能的研究级仪器设计为顶级线。上级 系统的功能对于我们开发和优化我们的工作流程和方法是必要的 用于特定于位置的IR光谱研究局部蛋白质动力学。我们的发现将极大地受益 研究人员使用IR方法用于大型，复杂的蛋白质。因此，请求的设备将有所帮助 父母项目对生物物理学和生物无机研究领域产生持续，强大的影响 化学。此外，行政补充剂将增强NIH资助的科学环境 新墨西哥大学的调查人员，包括药学院内外。向我们 知识，该地区的教育机构不存在可比的仪器。迪恩·戈德温（Dean Godwin） 已经为此补充要求提供了大量财政支持，因为这是一项巨大的投资 大学。总体而言，拟议的补充剂将增加父母奖在 我们机构的原始范围和增强了研究质量和深度。