Imaging Core

成像核心

基本信息

批准号：
7646824
负责人：
SIMON C WATKINS
金额：
$ 21.58万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7646824
关键词：
Adoptive Transfer Animals Apoptosis Area Arts Autoradiography Biological Biological Assay Biological Products Cell Communication Cell Death Cell Line Cells Clinical Collaborations Colorectal Cancer Complement Complementary DNA Computer Assisted Confocal Microscopy Consultations Data Documentation Electron Microscopy Electrons Fluorescence Fluorescence Microscopy Gene Expression Generations Genes Goals Hematopoietic Histology Human Image Image Analysis Imagery Imaging technology Immune Immune response Immune system Immunology Immunomodulators In Situ In Situ Hybridization In Vitro Individual Label Laboratories Lasers Life Light Location Luciferases Magnetic Resonance Imaging Malignant Glioma Malignant Neoplasms Measurement Messenger RNA Methods Microscopic Modeling Molecular Molecular Profiling Mus Natural Killer Cells Optical Methods Optical reporter Optics Pathology processes Pattern Play Process Productivity Proteins Publications Radioactive Reagent Reporter Genes Research Infrastructure Research Personnel Research Project Grants Resources Reverse Transcriptase Polymerase Chain Reaction Rodent Model Role Running Services Source Speed Staining method Stains T-Lymphocyte Techniques Technology Therapeutic Therapeutic Agents Time Tissues Training Transgenic Mice Transgenic Organisms Transmission Electron Microscopy Tumor Tissue Universities Vaccinia virus Viral Viral Genes base cRNA Probes cell killing cell motility cell type cellular imaging chemokine cost data sharing design experience image processing image visualization in vivo insight instrumentation mRNA Expression melanoma molecular imaging neoplastic cell novel strategies optical imaging population based pre-clinical research programs protein expression research study response tool trafficking tumor tumor progression

项目摘要

The central goal of this program is to understand the therapeutic utility of novel strategies to stimulate Tumor cell killing by the immune system. The Imaging Core will play multiple diverse roles within this program. In summary, our primary roles will be : careful analysis of cellular and molecular processes associated with apoptosis, characterization and quantification of cell types within tumors, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of protein expression by individual cells within tissues, and measurement of the levels and patterns of expression of chemokines and cell-type markers in tumors and tumor explants. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy, live cell imaging, transmission electron microscopy and computer-aided morphometric analyses. The Center for Biologic Imaging, in which this core (B1) service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project. Within the scope of this project at the light microscopic level these methods include: histology, immuno-histology, live cell and in situ hybridization methods. The Core will be used extensively by all projects. Core B2 will provide in situ analysis services to all projects, including: generation and sequence confirmation of gene-specific cDNA templates for probe synthesis; in situ hybridization with radioactive cRNA probes and autoradiography; combined in situ hybridization and immunohistochemical staining; and image capture, analysis, and documentation for data sharing and publication. These analysis services will provide integrated data on chemokine expression profiles and producer cells in tumor-containing tissues provided by all projects. Core B3, will act to provide whole animal MRI services as needed in each project. In the previous submission this core was very highly regarded as such changes have been kept to a minimum and only reflect continued interaction and productivity between the core and the project Pis RELEVANCE (Seeinstructions):

该计划的核心目标是了解刺激肿瘤的新型策略的治疗效用免疫系统杀死细胞。成像核心将在此程序中扮演多种不同的角色。在总而言之，我们的主要作用将是：仔细分析与相关的细胞和分子过程肿瘤内细胞类型的凋亡，表征和定量，检查蛋白质表达在细胞死亡期间，在体外检查细胞细胞相互作用，通过定量分析蛋白质表达组织中的单个细胞，以及测量趋化因子表达的水平和模式和肿瘤和肿瘤外植体中的细胞型标记。这些研究将采用一系列当前的光，以及可能的是电子显微镜方法，包括：单色和多色荧光显微镜，激光器共聚焦显微镜，活细胞成像，透射电子显微镜和计算机辅助形态计量学分析。该核心（B1）服务的生物成像中心是为此设计的为其用户提供最先进的微观技术的目的。它有能力执行光学方法的连续体，包括所有类型的光和电子显微镜，对此程序必不可少项目。在光学层面的该项目范围内，这些方法包括：组织学，免疫 - 源，活细胞和原位杂交方法。核心将被所有人广泛使用项目。核心B2将为所有项目提供原位分析服务，包括：发电和顺序确认基因特异性cDNA模板进行探针合成；与放射性CRNA的原位杂交探针和放射自显影；结合原位杂交和免疫组织化学染色；和图像捕获数据共享和出版物的捕获，分析和文档。这些分析服务将提供关于趋化因子表达谱和含肿瘤组织中趋化因子表达谱和生产细胞的综合数据所有项目。 Core B3将根据每个项目的需要提供全动物MRI服务。在以前的提交此核心被高度重视，因为此类更改已保持至最低限度，并且仅反映核心与项目PI之间的持续互动和生产力相关性（参见Instructions）：