Multiphon imaging for understanding social brain function in tadpoles

多声子成像用于了解蝌蚪的社交脑功能

基本信息

批准号：
10717610
负责人：
NA Ji
金额：
$ 63.65万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-15 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10717610
关键词：
3-Dimensional Ablation Address Afferent Neurons Amphibia Animals Area Atlases Behavior Brain Brain imaging Caregivers Cells Code Communication Communities Complex Cues Dancing Dangerousness Data Decision Making Development Fire - disasters Fluorescence Fluorescence Microscopy Foundations Future Generations Goals Hunger Image Imaging Device Imaging Techniques Individual Infant Label Larva Learning Life Longitudinal Studies Mammals Measurement Measures Methods Microscopy Modeling Mothers Motor Nature Neurons Neurophysiology - biologic function Neurosciences Norepinephrine Nutritional Odors Olfactory Learning Olfactory Pathways Optics Organism Parent-Child Relations Parents Pattern Performance Photons Pigmentation physiologic function Poison Protocols documentation Proxy Rana Research Resolution Siblings Signal Transduction Slice Smell Perception Social Behavior Social Environment Social Interaction Specificity Statistical Data Interpretation Stimulus Tadpoles Techniques Technology Testing Time Visual Visualization Xenopus adaptive optics comparative cranium experimental study feeding image reconstruction imaging approach imaging capabilities imprint improved in vivo in vivo imaging model organism multi-photon multiphoton imaging multiphoton microscopy neonate neural neural circuit neuroethology neuroimaging olfactory bulb olfactory sensory neurons pup response social social attachment tool two-photon

项目摘要

Project Summary Mother-infant bonding is a key relationship that lays a foundation for wellness throughout life. Social recognition is an important component of this relationship, as infants imprint on the smell of their mothers and use olfaction to distinguish their mother from others. This behavior is widespread in mammals, although the complexity of the mammalian brain and paucity of tools and repeated imaging capabilities to study neonate pups make it difficult to obtain a precise mechanistic understanding of the basic brain mechanisms for mother-infant bonding and communication in young. Mother-infant recognition and bonding also occur in other taxa, suggesting there are other species in which to study generalizable neural principles of parent-offspring interactions. Recently our labs have developed (1) poison frog tadpoles as a model to study social brain development and (2) multiphoton imaging approaches to enable in vivo recording of pigmented aquatic larvae. We propose to develop multiphoton in vivo imaging approaches to study the encoding of maternal odors in a social poison frog tadpole that can distinguish their mother from strangers. We hypothesize that the olfactory response landscape changes throughout development as tadpoles learn the smell of their mothers. We predict that a tadpole’s ability to distinguish their mother from strangers coincides with an increase in olfactory cells that fire with specificity to maternal odors, measured by multiphoton imaging of olfactory system neural activity repeatedly across development. Prior to these experiments, we will evaluate the performance of various multiphoton imaging techniques for optically accessible depth inside the brain of multiple species of poison frog tadpoles with varying levels of pigmentation. We will validate the results with rigorous statistical analyses and comparison of neural activity data with immunohistological imaging of brain slices and activity-dependent sequencing of olfactory sensory neurons. Understanding how amphibians learn and encode individual conspecific identity will either reveal alternative mechanisms of encoding olfactory-based recognition or which patterns of olfactory encoding are ancestral or generalizable features of vertebrate olfactory processing. Importantly, our approach will result in the development of multiphoton approaches for whole brain imaging of pigmented aquatic animals, which is a valuable toolkit of broad use for the neuroscience community. Successful completion of this project will allow us to obtain proof-of-principle data for proposing in vivo imaging of tadpole brains throughout development, in a comparative context, and within the framework of ethologically relevant behaviors, which is crucial for future R01 applications. Furthermore, establishing a recording protocol in tadpoles will allow for other aspects of neural function in amphibians, a research area that has thus far been limited due to technological constraints. In summary, our proposed research will help elucidate core olfactory imprinting principles in a relatively simple tadpole brain and reveal how these principles for olfactory encoding may generalize across vertebrate taxa.

项目摘要母亲纽带是一种关键关系，为整个生命奠定了基础。社会的识别是这种关系的重要组成部分，因为婴儿在母亲的气味和使用嗅觉将母亲与他人区分开。这种行为在哺乳动物中很普遍，尽管哺乳动物大脑的复杂性以及工具和重复成像能力的缺乏幼崽使得很难获得对基本脑机制的精确机械理解年轻的母亲结合和交流。母亲的识别和结合也发生在其他分类单元，表明还有其他物种可以研究父母源的概括神经原理互动。最近，我们的实验室开发了（1）毒蛙t作为研究社会大脑的模型开发和（2）多光子成像方法，以实现小猪幼虫的体内记录。我们建议开发体内成像方法的多光子，以研究在A中的母体气味的编码社交毒药青蛙t，可以将母亲与陌生人区分开。我们假设嗅觉随着t会学习母亲的气味，整个发展的响应景观变化。我们预测 t t的能力与陌生人区分开的能力与嗅觉细胞的增加一致通过对母体气味特异性的火灾，通过嗅觉系统神经活动的多光子成像来测量再次开发。在进行这些实验之前，我们将评估各种的性能多光子成像技术，用于在多种毒蛙大脑内的光学上可访问的深度 t的色素沉着水平变化。我们将通过严格的统计分析来验证结果，并神经活动数据与脑切片的免疫组织学成像和活动依赖性的比较嗅觉感觉神经元的测序。了解两栖动物如何学习和编码个人同种身份将揭示编码基于嗅觉识别的替代机制或嗅觉编码的模式是脊椎动物嗅觉加工的祖先或可推广的特征。重要的是，我们的方法将导致多光子方法的发展，以进行全脑成像养猪动物，这是神经科学界广泛使用的有价值的工具包。成功完成该项目将使我们能够获得用于在体内成像中提出的原理数据证明在整个发展，比较背景下以及在伦理上的框架内相关行为，这对于将来的R01应用至关重要。此外，建立录音协议在t中，将允许两栖动物的神经功能的其他方面，这是一个迄今为止的研究领域由于技术限制而受到限制。总而言之，我们提出的研究将有助于阐明核心嗅觉在相对简单的t脑大脑中烙印原理，并揭示这些嗅觉编码的原理如何可以概括跨脊椎动物类群。