Cell-type specific characterization of neuronal activity throughout V1

V1 期间神经元活动的细胞类型特异性特征

基本信息

批准号：
10438695
负责人：
NA Ji
金额：
$ 38.75万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10438695
关键词：
Automobile Driving Axon Behavioral Brain Calcium Cells Complex Data Data Analyses Data Science Core Data Scientist Data Set Data Storage and Retrieval Development Electrophysiology (science)Explosion Feedback Fluorescence Microscopy Functional Imaging Gene Expression Profiling Head Image Imaging technology In Situ In Vitro Investigation Location Measurement Measures Modeling Mus Neurons Optics Pattern Physiological Population Protocols documentation RNA Resolution Scanning Sensory Specificity Speed Stimulus Synapses Techniques Technology Testing Theoretical model Time V1 neuron Validation Work adaptive optics awake base calcium indicator cell type data analysis pipeline design experimental study imaging study in vivo in vivo imaging large scale data model development movie neural circuit neuronal cell body predictive modeling relating to nervous system response theories two-photon visual stimulus

Automobile Driving Axon Behavioral Brain Calcium Cells Complex Data Data Analyses Data Science Core Data Scientist Data Set Data Storage and Retrieval Development Electrophysiology (science)Explosion Feedback Fluorescence Microscopy Functional Imaging Gene Expression Profiling Head Image Imaging technology In Situ In Vitro Investigation Location Measurement Measures Modeling Mus Neurons Optics Pattern Physiological Population Protocols documentation RNA Resolution Scanning Sensory Specificity Speed Stimulus Synapses Techniques Technology Testing Theoretical model Time V1 neuron Validation Work adaptive optics awake base calcium indicator cell type data analysis pipeline design experimental study imaging study in vivo in vivo imaging large scale data model development movie neural circuit neuronal cell body predictive modeling relating to nervous system response theories two-photon visual stimulus

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项目摘要

SUMMARY To constrain and test models of V1, we need to comprehensively characterize the physiological responses of neurons as well as their main inputs in all six cortical layers. However, imaging neuronal cell bodies or axons deep in the cortex has remained a major technical challenge. The Ji lab made critical breakthroughs in applying adaptive optics to correct brain-induced optical aberrations for in vivo two-photon fluorescence microscopy, so that the activity of axons, boutons, and neurons can be captured across the full depth of cortex. Using Bessel focus scanning technology, the Ji lab also demonstrated high speed (30 Hz) volumetric calcium imaging with synaptic resolution in vivo. In awake mice and under different brain states, these technologies will be used to measure neural activity of both cortical neurons and their main sensory and modulatory inputs in response to a concerted set of visual stimuli including natural movies to rigorously test model predictions. Combined with subsequent in vitro functional connectivity mapping and post hoc cell type identification, we will generate, for the first time, datasets where every neuron within V1 is described by its location, responses to known stimuli, connectivity pattern, and cell type, to guide and validate the modeling projects.

概括为了限制V1的模型和测试模型，我们需要全面地表征神经元及其主要输入在所有六个皮层层中。但是，成像神经元细胞或轴突皮质深处仍然是一个重大的技术挑战。 JI实验室在应用自适应光学元件来纠正体内双光子荧光的脑诱导的光学畸变显微镜，因此可以在整个皮层深度捕获轴突，胸子和神经元的活性。使用Bessel Focus扫描技术，JI实验室还展示了高速（30 Hz）体积钙在体内具有突触分辨率的成像。在清醒的老鼠和不同的大脑状态下，这些技术将用于测量皮质神经元及其主要感觉和调节输入的神经活动对一组共同的视觉刺激的响应，包括自然电影，以严格测试模型预测。结合随后的体外功能连通性映射和事后事后细胞类型识别，我们将生成第一次生成数据集，其中V1中的每个神经元的位置都描述了已知的刺激，连通性模式和细胞类型，以指导和验证建模项目。