Continued support for an SPF Macaca nemestrina breeding colony to provide animals to NIH funded HIV research projects

继续支持 SPF 猕猴繁殖群，为 NIH 资助的艾滋病毒研究项目提供动物

• 批准号: 10403106
• 负责人: Eric Kenneth Hutchinson
• 金额: $ 102.17万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10403106
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Address; Adolescent; Animal Sources; Animals; Area; Behavioral; Biological Assay; Breeding; Cercopithecine Herpesvirus 1; Country; Disease; Female; Funding; Genome; Genomic approach; Genotype; Goals; Grant; HIV; Histocompatibility; Institution; Life; Macaca; Macaca nemestrina; Major Histocompatibility Complex; Modeling; Population; Primates; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; SIV; Sales; Serology; Simian Retroviruses; Testing; U-Series Cooperative Agreements; United States; United States National Institutes of Health; Universities; Viral; Virus; animal breeding; deep sequencing; functional genomics; genetic pedigree; germ free condition; health data; improved; interest; male; offspring; operation; preservation; screening

Abstract The aims of this project are to provide healthy, behaviorally normal, MHC-characterized, SPF pig-tailed macaques for NIH-funded HIV/AIDS related research. We will expand the size of the current SPF breeding colony to increase the numbers of females for breeding and juvenile males and females available for sale to NIH-funded HIV/AIDS investigators both within Johns Hopkins University and at other research institutions. Complete pedigrees and health data are maintained and provided to investigators when animals are being selected for sale. Viral screening includes regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV), and Simian T- lymphotrophic Virus (STLV). For the past decade the colony has consistently and repeatedly tested negative for these agents. Major histocompatibility (MHC) profiles are performed on all juveniles during the first year of life and are provided to investigators when animals are being selected for purchase. Over the last five years, the colony has produced an average of 51 offspring each year, resulting in an average of 32 animals sold. We estimate that selling 42 animals per year would allow the colony to achieve self-sufficiency, a goal we project we will reach by the end of 2028.

抽象的 该项目的目的是提供健康，行为正常的，由MHC特征，SPF猪尾提供的 NIH资助的HIV/AIDS相关研究的猕猴。我们将扩大当前SPF繁殖的大小 殖民地以增加可供出售的繁殖和少年男性和雌性的女性人数 约翰·霍普金斯大学和其他研究机构的NIH资助的艾滋病毒/艾滋病调查员。 在动物时，将保持完整的血统和健康数据并提供给调查人员 选择出售。病毒筛查包括4种病毒剂的常规血清学和PCR测试：Cercopithecine 疱疹病毒1（B病毒），邻肌免疫缺陷病毒（SIV），Simian逆转录病毒（SRV）和Simian T- 淋巴营养病毒（STLV）。在过去的十年中，殖民地一直始终如一地测试了阴性 对于这些代理。在第一年，对所有少年进行了主要的组织相容性（MHC）剖面 当选择动物购买时，生命并提供给调查人员。在过去的五年中， 该殖民地平均每年产生51个后代，平均售出32只动物。我们 估计每年出售42只动物将使殖民地达到自给自足，这是我们计划的目标 我们将在2028年底到达。