Viral Testing Core

病毒检测核心

• 批准号: 10576957
• 负责人: Eric Kenneth Hutchinson
• 金额: $ 11.58万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10576957
• 关键词: Animals; Biological Assay; Biomedical Research; Breeding; Cercopithecine Herpesvirus 1; Communities; Development; Funding; Goals; HIV; HIV/AIDS; Infant; Laboratories; Life; Macaca; Macaca nemestrina; Maintenance; Modeling; Primates; Quarantine; Reproducibility; Research; Research Project Grants; Resources; SIV; Serology; Simian Retroviruses; Source; Supervision; Testing; United States National Institutes of Health; Universities; Viral; Virus; germ free condition

Abstract To enhance the rigor and reproducibility of SIV/macaque models of HIV/AIDS, it is essential to provide specific pathogen free (SPF) macaques to the research community. The NIH/ORIP has supported the development and maintenance of SPF macaque colonies since 2000 as a critical biomedical research resource particularly for SIV/macaque models of HIV/AIDS. Every pigtail macaque currently in the Johns Hopkins University SPF breeding colony meets the 4 viral agent SPF criteria set by NIH/ORIP, testing negative for Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1). The SPF status of the JHU pigtail macaque breeding colony has been maintained for over ten years with no breaks detected by ongoing surveillance. The Virus Reference Laboratory (VRL, San Antonio, TX) performs all of our primate serology and PCR assays. Under the supervision of Dr. Eric Hutchinson, core director, all of the pigtail macaques at Johns Hopkins are tested annually by serology for the four SPF viral agents. In addition, all colony-born infants undergo two sequential PCR tests for SRV and STLV during the first year of life. Any rare additions to the colony from sources outside JHU are similarly tested by repeat PCR upon arrival in JHU quarantine. Our goal is to continue maintenance of SPF status of a valuable macaque national research resource for HIV/AIDS research. The specific aim of this Viral Testing Core is to maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV-1).

抽象的 为了增强艾滋病毒/艾滋病的SIV/猕猴模型的严格性和可重复性，至关重要 向研究界提供特定的无病原体（SPF）猕猴。 NIH/ORIP具有 自2000年以来支持SPF猕猴殖民地的发展和维护作为关键 生物医学研究资源，尤其是针对艾滋病毒/艾滋病的SIV/猕猴模型。每个辫子 目前在约翰·霍普金斯大学SPF育种殖民地的猕猴遇到了4个病毒毒剂 NIH/ORIP设定的SPF标准，测试Cercopithecine疱疹病毒1（B病毒），测试阴性 猿猴免疫缺陷病毒（SIV），猿猴逆转录病毒（SRV）和猿猴T-淋巴营 病毒（STLV-1）。 JHU辫子猕猴繁殖菌落的SPF状态一直是 维持了十多年，没有通过正在进行的监视检测到的休息时间。病毒 参考实验室（VRL，德克萨斯州圣安东尼奥）执行我们所有的灵长类动物血清学和PCR 测定。在所有辫子猕猴的核心主任埃里克·哈钦森（Eric Hutchinson）的监督下 在约翰·霍普金斯（Johns Hopkins），每年通过血清学对四种SPF病毒剂进行测试。另外，所有人 出生于菌落的婴儿在第一年对SRV和STLV进行了两次顺序PCR测试 生活。同样，JHU以外的殖民地的任何罕见添加都可以通过重复测试 PCR到达Jhu Preactine。我们的目标是继续维护A的SPF状态 宝贵的猕猴国家研究资源用于艾滋病毒/艾滋病研究。这个的具体目的 病毒测试核心是通过常规血清学和PCR维持菌落的SPF状态 对4种病毒剂的测试：Cercopithecine疱疹病毒1（B病毒），猿猴免疫缺陷 病毒（SIV），猿猴逆转录病毒（SRV）和Simian T淋巴营养病毒（STLV-1）。