Role of the Gut Microbiome in Mosquito Development

肠道微生物组在蚊子发育中的作用

• 批准号: 9804977
• 负责人: Michael Strand
• 金额: $ 35.5万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-16 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9804977
• 关键词: Adult; Aedes; Affect; Alternative Splicing; Anopheles gambiae; Arthropod Vectors; Award; Bacteria; Bacterial Genes; Biological Assay; Biology; Blood; Cell Proliferation; Cell Respiration; Communities; Complex; Consumption; Culicidae; Development; Diet; Disease; Disease Vectors; Environment; Escherichia coli; Exhibits; Fat Body; Female; Foundations; Funding; Gastrointestinal tract structure; Gene Activation; Genes; Genetic Screening; Gnotobiotic; Goals; Gram-Negative Bacteria; Growth; High-Throughput Nucleotide Sequencing; Human; Hypoxia; Individual; Intestines; Invertebrates; Laboratory culture; Larva; Length; Link; Lipids; Measures; Mediating; Metabolism; Methods; Microbe; Midgut; Molecular; Molecular Target; Molting; Nutrient; Nutritional; Organism; Outcome; Oxygen; Pathway interactions; Physiology; Population; Process; Progress Reports; Protein Isoforms; Quality Control; Regulatory Pathway; Reporting; Resources; Risk; Role; SECTM1 gene; Series; Signal Pathway; Signal Transduction; Source; Stem cells; Structure; Tissues; Vertebrates; Work; activating transcription factor; base; chromatin immunoprecipitation; disorder control; experimental study; feeding; fitness; gut microbes; gut microbiome; gut microbiota; human disease; improved; innovation; insight; lipid biosynthesis; member; pathogen; programs; transcription factor; transmission process; vector; vector control; vector mosquito

Project Summary Mosquitoes are well recognized as the most important arthropod vectors of disease-causing pathogens. This is because most species must feed on vertebrate blood as adult females to reproduce. In turn, consumption of multiple blood meals enables pathogen transmission between hosts while vector abundance influences transmission risks. Thus, approaches that disrupt pathogen transmission and/or reduce vector populations are both strategies for disease control. Mosquitoes host microbes in their digestive tract that collectively form a gut microbiota. This application is a renewal request to continue our work on the essential role of the gut microbiota in mosquito development. Our current award focuses on Aedes aegypti, which is a major vector of several human diseases. Our studies to date indicate that microbe- induced gut hypoxia functions as a growth signal, and that transduction of this signal in mosquito larvae requires hypoxia-induced transcription factors (HIFs). Results further indicate that HIF signaling activates several processes with essential roles in nutrient acquisition, growth and metabolism. The overall goal of this proposal is to unravel the molecular mechanisms that connect microbe-induced gut hypoxia and HIF signaling to the nutrients and signaling pathways that regulate growth. Proposed studies have the potential for transformative impacts on understanding the role of the gut microbiota in development of most if not all vector mosquito species. Proposed Specific Aims are to: 1. Identify HIF target genes in Ae. aegypti. 2. Characterize Ae. aegypti HIF-α isoforms. 3. Analyze the role of HIF signaling in lipogenesis and midgut growth. 4. Assess the role of the gut microbiota as nutrient source for mosquito development. Expected outcomes will identify molecular targets for disrupting mosquito development and factors that could improve mosquito rearing for control programs. Results will also guide understanding of how diet and the gut microbiota regulate growth of all mosquito species.

项目摘要 蚊子被广泛认为是最重要的致病性节肢动物载体 病原体。这是因为大多数物种必须以脊椎动物为食，因为成年女性 复制。反过 在宿主之间，矢量抽象会影响传输风险。那就接近了 破坏病原体的传播和/或减少矢量种群都是 疾病控制。蚊子在其消化道中托管微生物，共同形成肠道 微生物群。该申请是一个续签请求，以继续我们的工作 蚊子发育中的肠道菌群。我们目前的奖项专注于埃及埃及的奖项， 这是几种人类疾病的主要媒介。迄今为止，我们的研究表明微生物 - 诱导的肠道缺氧是生长信号，并且该信号在 蚊子幼虫需要缺氧诱导的转录因子（HIF）。结果进一步指示 HIF信号传导在营养习得中激活多个过程， 生长和代谢。该提议的总体目标是揭开分子 将微生物诱导的肠道缺氧和HIF信号传导与养分和营养物质的机制 调节生长的信号通路。拟议的研究有可能 对了解肠道微生物群在大多数发展中的作用的变革性影响 如果不是全部，媒介蚊子的规格。建议的具体目的是： 1。识别AE中的HIF靶基因。埃及。 2。代表AE。埃及HIF-α同工型。 3。分析HIF信号在脂肪生成和中肠生长中的作用。 4。评估肠道菌群作为蚊子发育的营养来源的作用。 预期的结果将确定破坏蚊子发展的分子靶标的 可以改善控制程序的蚊子饲养的因素。结果也将指导 了解饮食和肠道微生物群如何调节所有蚊子的生长。