Development of a novel gel bait for the control of mosquitoes in urban environments

开发用于控制城市环境中蚊子的新型凝胶诱饵

• 批准号: 10478531
• 负责人: Dangsheng Liang
• 金额: $ 24.99万
• 依托单位: APEX BAIT TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-06 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10478531
• 关键词: Adult; Aedes; Affect; Aging; Anopheles Genus; Behavior; Biological; Biological Assay; Blood; Blood Glucose; Blood Substitutes; Boric Acids; Centers for Disease Control and Prevention (U.S.); Chemicals; Chikungunya virus; Consumption; Coupled; Culex pipiens; Culicidae; Dengue Virus; Development; Disease; Disease Outbreaks; Disease Surveillance; Effectiveness; Environment; Feeding behaviors; Formulation; Gel; Goals; Head; Hour; Human; Individual; Insect Control; Insecta; Insecticide Resistance; Insecticides; Laboratories; Lead; Life; Malaria; Measures; Methods; Monitor; Mosquito Control; Mosquito-borne infectious disease; Oral; Palate; Performance; Phase; Population; Preparation; Process; Production; Public Health; Reporting; Research; Residual state; Resistance development; Risk; Role; Semiochemicals; Small Business Innovation Research Grant; Solid; Speed; System; Technology; Testing; Toxic effect; Urban Community; Urban Population; Urbanization; West Nile virus; Work; Zika Virus; base; burden of illness; combat; commercialization; disease transmission; disorder control; economic cost; efficacy testing; experience; feeding; field study; human pathogen; improved; mortality; novel; prototype; public health relevance; research and development; response; screening; stability testing; sugar; survivorship; urban area; urban setting; vector; vector mosquito

Project Summary Mosquito-vectored disease outbreaks are on the rise in the USA (4,858 in 2004 to 47,461 in 2016, CDC: National notifiable disease surveillance system). The increased incidents of the human cases of dengue virus, West Nile virus, and chikungunya virus among urban populations indicate the role of expanding urbanization in facilitating the production of disease-transmitting mosquitoes. Our proposal aims to develop a novel semiochemical-based, blood-substitute gel bait to improve monitoring and control strategies for a variety of mosquito species in our nation's urban communities. Bait has become the preferred method of control against many urban insect pests due to its economic cost, ease of deployment, and superior efficacy coupled with minimal impact on public health, environment, and non-target species. Despite this, bait technologies targeting mosquitoes are lacking, as their unique feeding behavior makes the use of typical solid baits difficult. Even the highly investigated attractive toxic sugar baits (ATSB) targeting adult mosquitoes have failed to yield an effective commercial product. This SBIR project proposes the development of a blood substitute gel bait that will exploit both the blood and sugar-feeding behavior of mosquitoes instead of only sugar feeding behavior as in ATSB baits. Specifically, we hypothesize that a bait matrix based on a combination of blood and sugar meal components, combined with attractants, phagostimulants, and insecticides could effectively overcome many of the pitfalls of current chemical control methods and result in an effective commercial product that can be used in the urban setting for mosquito control. Our research team recently reported that a prototype blood-substitute gel bait containing attractants, phagostimulants, and insecticides outperforms the only commercially available mosquito ATSB product in head-to-head mosquito attraction and mortality comparisons. Accordingly, we predict that targeting the blood-feeding behavior of mosquitos by incorporating long-distance attractants and reduced risk insecticide in gel bait formulations would be highly successful in reducing mosquito populations in urban settings. We propose three specific aims to test the technical feasibility of this approach for mosquito control. In aim 1, we will determine if the addition of long-distance attractants can further increase mosquito attraction to the gel bait. In aim 2, we will evaluate the performance of boric acid and spinosad as reduced risk bait insecticides against three different species of mosquito. In aim 3, we will test the efficacy of prototype bait containing newly identified attractants and insecticide (from aim 1 and 2) in semi-field test and determine the environmental stability of gel bait to access indoor and outdoor field life of this product. The results of our work will build on Apex Bait Technologies' recent accumulated research on host attractants and blood phagostimulants as well as on decade-long experience in research, development, and commercialization of bait technologies for urban pests.

项目摘要 美国蚊子向量的疾病暴发在美国正在上升（2004年4,858次，2016年的47,461，CDC： 国家通知疾病监测系统）。人类登革热病毒病例的事件增加， 西尼罗河病毒和城市种群中的基孔肯雅病毒表明了扩大城市化的作用 促进疾病传播蚊子的产生。我们的建议旨在发展小说 基于半化学的基于血液杀菌的凝胶诱饵，以改善各种监测和控制策略 我们国家城市社区中的蚊子物种。诱饵已成为控制的首选方法 许多城市害虫由于其经济成本，易于部署和卓越的效力而与之相结合 对公共卫生，环境和非目标物种的影响最小。尽管如此，诱饵技术针对 缺乏蚊子，因为它们的独特喂养行为使使用典型的固体诱饵困难。甚至是 靶向成人蚊子的高度研究的有吸引力的有毒糖诱饵（ATSB）未能产生 有效的商业产品。这个SBIR项目提议开发血液替代凝胶诱饵 将利用蚊子的血液和喂食行为，而不是仅仅 在ATSB诱饵中。具体而言，我们假设基于血液和糖粉的组合的诱饵基质 组件，加上吸引剂，吞噬量和杀虫剂可以有效地克服许多 当前化学控制方法的陷阱，并导致有效的商业产品可以使用 在蚊子控制的城市环境中。我们的研究小组最近报道了原型的血液替代 凝胶诱导剂，吞噬刺激剂和杀虫剂的表现优于唯一可用的 蚊子ATSB产品以蚊子吸引力和死亡率比较。因此，我们 预测，通过结合长距离吸引者和 凝胶诱饵配方中的风险杀虫剂降低将在减少蚊子种群中非常成功 城市环境。我们提出了三个特定的目的，以测试这种方法的技术可行性 控制。在AIM 1中，我们将确定添加长距离吸引者是否可以进一步增加蚊子 吸引凝胶饵。在AIM 2中，我们将评估硼酸和Spinosad的性能，以降低风险 诱饵杀虫剂针对三种不同的蚊子。在AIM 3中，我们将测试原型诱饵的功效 在半场测试中包含新鉴定的吸引剂和杀虫剂（来自AIM 1和2），并确定 凝胶诱饵可进入该产品的室内和室外现场寿命的环境稳定性。我们工作的结果 将基于Apex Bait Technologies最近对宿主吸引剂和血液的累积研究 在研究，开发和商业化的长达十年经验中 城市害虫的诱饵技术。