Development of a novel gel bait for the control of mosquitoes in urban environments

开发用于控制城市环境中蚊子的新型凝胶诱饵

• 批准号: 10478531
• 负责人: Dangsheng Liang
• 金额: $ 24.99万
• 依托单位: APEX BAIT TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-06 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10478531
• 关键词: Adult; Aedes; Affect; Aging; Anopheles Genus; Behavior; Biological; Biological Assay; Blood; Blood Glucose; Blood Substitutes; Boric Acids; Centers for Disease Control and Prevention (U.S.); Chemicals; Chikungunya virus; Consumption; Coupled; Culex pipiens; Culicidae; Dengue Virus; Development; Disease; Disease Outbreaks; Disease Surveillance; Effectiveness; Environment; Feeding behaviors; Formulation; Gel; Goals; Head; Hour; Human; Individual; Insect Control; Insecta; Insecticide Resistance; Insecticides; Laboratories; Lead; Life; Malaria; Measures; Methods; Monitor; Mosquito Control; Mosquito-borne infectious disease; Oral; Palate; Performance; Phase; Population; Preparation; Process; Production; Public Health; Reporting; Research; Residual state; Resistance development; Risk; Role; Semiochemicals; Small Business Innovation Research Grant; Solid; Speed; System; Technology; Testing; Toxic effect; Urban Community; Urban Population; Urbanization; West Nile virus; Work; Zika Virus; base; burden of illness; combat; commercialization; disease transmission; disorder control; economic cost; efficacy testing; experience; feeding; field study; human pathogen; improved; mortality; novel; prototype; public health relevance; research and development; response; screening; stability testing; sugar; survivorship; urban area; urban setting; vector; vector mosquito

Project Summary Mosquito-vectored disease outbreaks are on the rise in the USA (4,858 in 2004 to 47,461 in 2016, CDC: National notifiable disease surveillance system). The increased incidents of the human cases of dengue virus, West Nile virus, and chikungunya virus among urban populations indicate the role of expanding urbanization in facilitating the production of disease-transmitting mosquitoes. Our proposal aims to develop a novel semiochemical-based, blood-substitute gel bait to improve monitoring and control strategies for a variety of mosquito species in our nation's urban communities. Bait has become the preferred method of control against many urban insect pests due to its economic cost, ease of deployment, and superior efficacy coupled with minimal impact on public health, environment, and non-target species. Despite this, bait technologies targeting mosquitoes are lacking, as their unique feeding behavior makes the use of typical solid baits difficult. Even the highly investigated attractive toxic sugar baits (ATSB) targeting adult mosquitoes have failed to yield an effective commercial product. This SBIR project proposes the development of a blood substitute gel bait that will exploit both the blood and sugar-feeding behavior of mosquitoes instead of only sugar feeding behavior as in ATSB baits. Specifically, we hypothesize that a bait matrix based on a combination of blood and sugar meal components, combined with attractants, phagostimulants, and insecticides could effectively overcome many of the pitfalls of current chemical control methods and result in an effective commercial product that can be used in the urban setting for mosquito control. Our research team recently reported that a prototype blood-substitute gel bait containing attractants, phagostimulants, and insecticides outperforms the only commercially available mosquito ATSB product in head-to-head mosquito attraction and mortality comparisons. Accordingly, we predict that targeting the blood-feeding behavior of mosquitos by incorporating long-distance attractants and reduced risk insecticide in gel bait formulations would be highly successful in reducing mosquito populations in urban settings. We propose three specific aims to test the technical feasibility of this approach for mosquito control. In aim 1, we will determine if the addition of long-distance attractants can further increase mosquito attraction to the gel bait. In aim 2, we will evaluate the performance of boric acid and spinosad as reduced risk bait insecticides against three different species of mosquito. In aim 3, we will test the efficacy of prototype bait containing newly identified attractants and insecticide (from aim 1 and 2) in semi-field test and determine the environmental stability of gel bait to access indoor and outdoor field life of this product. The results of our work will build on Apex Bait Technologies' recent accumulated research on host attractants and blood phagostimulants as well as on decade-long experience in research, development, and commercialization of bait technologies for urban pests.

项目概要 美国蚊媒疾病爆发呈上升趋势（2004 年为 4,858 起，2016 年为 47,461 起），CDC： 国家法定传染病监测系统）。人类感染登革热病毒病例的增加， 城市人口中的西尼罗河病毒和基孔肯雅病毒表明扩大城市化在 促进传播疾病的蚊子的产生。我们的建议旨在开发一种新颖的 基于化学信息素的血液替代凝胶诱饵，可改善多种疾病的监测和控制策略 我国城市社区的蚊子种类。诱饵已成为防治的首选方法 由于其经济成本、易于部署和卓越的功效，加上 对公共卫生、环境和非目标物种的影响最小。尽管如此，诱饵技术仍针对 蚊子的数量很少，因为它们独特的摄食行为使得典型的固体诱饵的使用变得困难。甚至连 针对成年蚊子的经过深入研究的有吸引力的有毒糖诱饵（ATSB）未能产生效果 有效的商业产品。该 SBIR 项目提议开发一种血液替代凝胶诱饵， 将利用蚊子的血液和糖喂养行为，而不是仅利用糖喂养行为 在 ATSB 诱饵中。具体来说，我们假设基于血液和糖粉组合的诱饵基质 成分与引诱剂、诱食剂和杀虫剂相结合，可以有效克服许多 当前化学控制方法的缺陷并产生了可以使用的有效商业产品 在城市环境中控制蚊子。我们的研究小组最近报告了一种原型血液替代品 含有引诱剂、诱食剂和杀虫剂的凝胶诱饵优于唯一市售的诱饵 蚊子 ATSB 产品进行头对头蚊子吸引力和死亡率比较。据此，我们 预测通过结合远距离引诱剂来瞄准蚊子的吸血行为 凝胶诱饵配方中的低风险杀虫剂将非常成功地减少蚊子数量 城市环境。我们提出了三个具体目标来测试这种方法对蚊子的技术可行性 控制。在目标1中，我们将确定添加远距离引诱剂是否可以进一步增加蚊子的数量 对凝胶诱饵的吸引力。在目标 2 中，我们将评估硼酸和多杀菌素降低风险的性能 针对三种不同种类的蚊子的诱饵杀虫剂。在目标3中，我们将测试原型诱饵的功效 在半田试验中含有新鉴定的引诱剂和杀虫剂（来自目标 1 和 2）并确定 胶饵的环境稳定性能进入本产品的室内外野外生活。我们的工作成果 将建立在 Apex Bait Technologies 最近积累的关于宿主引诱剂和血液的研究的基础上 诱食剂以及数十年的研究、开发和商业化经验 城市害虫诱饵技术。