Role of the Gut Microbiome in Mosquito Development

肠道微生物组在蚊子发育中的作用

• 批准号: 8816312
• 负责人: Michael Strand
• 金额: $ 37.35万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-16 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8816312
• 关键词: Address; Adult; Aedes; Aerobic; Affect; Algae; Arthropod Vectors; Bacteria; Bacterial Genes; Biological Assay; Biology; Biota; Blood; Communities; Culicidae; Data; Dependence; Development; Digestion; Disease; Egg Yolk Proteins; Escherichia coli; Exhibits; Female; Foundations; Gastrointestinal tract structure; Gene Expression Profile; Genes; Genome; Gnotobiotic; Goals; Growth; Human; Invertebrates; Laboratories; Laboratory culture; Larva; Libraries; Life; Link; Literature; Mammals; Methods; Microbe; Midgut; Mosquito Control; Nutrient; Oocytes; Outcome; Pathway interactions; Physiology; Play; Process; Recombinant DNA; Reporting; Reproduction; Role; Site; Source; Staging; Surveys; Vertebrates; Work; comparative; deep sequencing; disease transmission; egg; emerging adult; feeding; gut microbiota; insight; interest; knockout gene; member; microbial community; microbiome; microorganism; mutant; novel strategies; pathogen; public health relevance; research study; screening; sex; sugar; vector; vector control

DESCRIPTION (provided by applicant): Mosquitoes are well recognized as the most important arthropod vectors of disease-causing pathogens. Thus, studies on processes with potential to disrupt mosquito development or disease transmission are of biomedical importance. This application is a resubmission request to build on recent results showing that the gut micro biota of Aedes aegypti and other mosquito species are essential for development. It has long been known that the gut harbors bacteria but the role these microbes play in mosquito biology is largely unclear. Here we report preliminary data showing that the community of bacteria in the digestive tract of the mosquito Aedes aegypti is relatively simple but also exhibits distinct changes during development. Our results further show that bacteria-free (axenic) Aedes aegypti cannot develop, while recolonization of the gut by several gut community members and the non-community member Escherichia coli rescue development. Comparative data with other species yield similar results, which strongly suggests a fundamental, but heretofore unrecognized, dependence by most if not all mosquitoes on their gut microbiome for development. Recent studies identify bacterial genes with roles in developmental rescue as well as insights into how bacteria affect mosquito physiology. To further advance these results, we propose three specific aims. 1. Complete characterization of the gut micro biota in our laboratory cultures of Ae. aegypti and select other species, and conduct parallel experiments in field collected mosquitoes. 2. Further characterize E. coli mutants defective in rescuing mosquito development. 3. Analyze how bacteria affect mosquito physiology. Expected outcomes of our work will enhance understanding of the mosquito gut microbiome and its functions. Our study will impact the field of vector biology by providing information on the largely unknown but critical role gut microbes play in mosquito development. Our study will also provide information of potential importance in manipulating mosquito development as a strategy for vector control.

描述（由申请人提供）：蚊子被公认为是致病病原体最重要的节肢动物载体。因此，对可能破坏蚊子发育或疾病传播的过程的研究具有生物医学重要性。本申请是一项重新提交请求，以最近的结果为基础，表明埃及伊蚊和其他蚊子物种的肠道微生物群对于发育至关重要。人们早就知道肠道里含有细菌，但这些微生物在蚊子生物学中发挥的作用很大程度上还不清楚。在这里，我们报告的初步数据显示，埃及伊蚊消化道中的细菌群落相对简单，但在发育过程中也表现出明显的变化。我们的结果进一步表明，无菌（无菌）埃及伊蚊无法发育，而肠道菌群中的几个成员和非菌群成员大肠杆菌的重新定殖可以挽救发育。与其他物种的比较数据得出了类似的结果，这强烈表明大多数（如果不是全部）蚊子的发育依赖于其肠道微生物组，这是一种基本的、但迄今为止尚未认识到的依赖。最近的研究确定了细菌基因在发育救援中的作用，并深入了解细菌如何影响蚊子的生理机能。为了进一步推进这些成果，我们提出了三个具体目标。 1. 我们实验室的伊蚊培养物中肠道微生物群的完整表征。并选择其他物种，在野外采集的蚊子上进行平行实验。 2. 进一步表征在拯救蚊子发育方面存在缺陷的大肠杆菌突变体。 3. 分析细菌如何影响蚊子的生理机能。我们工作的预期成果将增强对蚊子肠道微生物组及其功能的了解。我们的研究将通过提供有关肠道微生物在蚊子发育中所发挥的很大程度上未知但关键作用的信息来影响媒介生物学领域。我们的研究还将提供对于控制蚊子发育作为病媒控制策略具有潜在重要性的信息。