Role of the Gut Microbiome in Mosquito Development

肠道微生物组在蚊子发育中的作用

• 批准号: 8816312
• 负责人: Michael Strand
• 金额: $ 37.35万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-16 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8816312
• 关键词: Address; Adult; Aedes; Aerobic; Affect; Algae; Arthropod Vectors; Bacteria; Bacterial Genes; Biological Assay; Biology; Biota; Blood; Communities; Culicidae; Data; Dependence; Development; Digestion; Disease; Egg Yolk Proteins; Escherichia coli; Exhibits; Female; Foundations; Gastrointestinal tract structure; Gene Expression Profile; Genes; Genome; Gnotobiotic; Goals; Growth; Human; Invertebrates; Laboratories; Laboratory culture; Larva; Libraries; Life; Link; Literature; Mammals; Methods; Microbe; Midgut; Mosquito Control; Nutrient; Oocytes; Outcome; Pathway interactions; Physiology; Play; Process; Recombinant DNA; Reporting; Reproduction; Role; Site; Source; Staging; Surveys; Vertebrates; Work; comparative; deep sequencing; disease transmission; egg; emerging adult; feeding; gut microbiota; insight; interest; knockout gene; member; microbial community; microbiome; microorganism; mutant; novel strategies; pathogen; public health relevance; research study; screening; sex; sugar; vector; vector control; Address; Adult; Aedes; Aerobic; Affect; Algae; Arthropod Vectors; Bacteria; Bacterial Genes; Biological Assay; Biology; Biota; Blood; Communities; Culicidae; Data; Dependence; Development; Digestion; Disease; Egg Yolk Proteins; Escherichia coli; Exhibits; Female; Foundations; Gastrointestinal tract structure; Gene Expression Profile; Genes; Genome; Gnotobiotic; Goals; Growth; Human; Invertebrates; Laboratories; Laboratory culture; Larva; Libraries; Life; Link; Literature; Mammals; Methods; Microbe; Midgut; Mosquito Control; Nutrient; Oocytes; Outcome; Pathway interactions; Physiology; Play; Process; Recombinant DNA; Reporting; Reproduction; Role; Site; Source; Staging; Surveys; Vertebrates; Work; comparative; deep sequencing; disease transmission; egg; emerging adult; feeding; gut microbiota; insight; interest; knockout gene; member; microbial community; microbiome; microorganism; mutant; novel strategies; pathogen; public health relevance; research study; screening; sex; sugar; vector; vector control

DESCRIPTION (provided by applicant): Mosquitoes are well recognized as the most important arthropod vectors of disease-causing pathogens. Thus, studies on processes with potential to disrupt mosquito development or disease transmission are of biomedical importance. This application is a resubmission request to build on recent results showing that the gut micro biota of Aedes aegypti and other mosquito species are essential for development. It has long been known that the gut harbors bacteria but the role these microbes play in mosquito biology is largely unclear. Here we report preliminary data showing that the community of bacteria in the digestive tract of the mosquito Aedes aegypti is relatively simple but also exhibits distinct changes during development. Our results further show that bacteria-free (axenic) Aedes aegypti cannot develop, while recolonization of the gut by several gut community members and the non-community member Escherichia coli rescue development. Comparative data with other species yield similar results, which strongly suggests a fundamental, but heretofore unrecognized, dependence by most if not all mosquitoes on their gut microbiome for development. Recent studies identify bacterial genes with roles in developmental rescue as well as insights into how bacteria affect mosquito physiology. To further advance these results, we propose three specific aims. 1. Complete characterization of the gut micro biota in our laboratory cultures of Ae. aegypti and select other species, and conduct parallel experiments in field collected mosquitoes. 2. Further characterize E. coli mutants defective in rescuing mosquito development. 3. Analyze how bacteria affect mosquito physiology. Expected outcomes of our work will enhance understanding of the mosquito gut microbiome and its functions. Our study will impact the field of vector biology by providing information on the largely unknown but critical role gut microbes play in mosquito development. Our study will also provide information of potential importance in manipulating mosquito development as a strategy for vector control.

描述（由申请人提供）：蚊子被广泛认为是最重要的致病病原体节肢动物载体。因此，对破坏蚊子发展或疾病传播潜力的过程的研究至关重要。该应用程序是基于最新结果的重新提出的请求，表明伊德斯伊德（Aedes）和其他蚊子物种的肠道微生物对开发至关重要。长期以来，众所周知，肠道细菌，但是这些微生物在蚊子生物学中的作用在很大程度上不清楚。在这里，我们报告了初步数据，表明蚊子埃及埃及消化道中的细菌群落相对简单，但在开发过程中也会显示出明显的变化。我们的结果进一步表明，无细菌（Axenic）埃及埃及无法发展，而几个肠道社区成员和非社区成员Escherichia Coli救援开发的肠道重新殖民化。与其他物种的比较数据产生了相似的结果，这强烈表明了基本的，但迄今未被认可的，大多数蚊子（如果不是全部）蚊子对其肠道微生物组进行发育的依赖性。最近的研究确定了在发育救援中作用的细菌基因，以及对细菌如何影响蚊子生理学的见解。为了进一步推进这些结果，我们提出了三个具体目标。 1。在AE实验室培养物中，肠道微生物的完全表征。埃及和选择其他物种，并在收集的蚊子中进行平行实验。 2。进一步描述了大肠杆菌突变体在营救蚊子开发方面有缺陷。 3。分析细菌如何影响蚊子生理。我们工作的预期结果将增强对蚊子肠道微生物组及其功能的理解。我们的研究将通过提供有关肠道微生物在蚊子开发中发挥的很大知名但至关重要的作用的信息来影响媒介生物学领域。我们的研究还将提供在操纵蚊子发展作为媒介控制策略中潜在重要性的信息。