Marker development for mosquito hemocytes

蚊子血细胞标记物开发

基本信息

批准号：
7018523
负责人：
Michael Strand
金额：
$ 16.72万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-03-01 至 2008-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7018523
关键词：
Anopheles arthropod genetics cell differentiation cell line cell morphology cell population study cellular immunity disease vectors hemolymph immunogenetics immunologic receptors phagocytosis protein structure function proteomics

项目摘要

DESCRIPTION (provided by applicant): The insect immune system consists of both cellular and humoral elements that innately recognize broad classes of foreign intruders. While great progress has been made over the last several years in identifying humoral factors like antimicrobial peptides and the signaling pathways that regulate their synthesis, much less is known about control of cellular defense responses. This is due in large part to the small size of many insects which makes collection of hemocytes and identification of hemocyte-produced effector molecules difficult. It is also often difficult to conduct manipulative experiments on hemocyte-mediated defense responses in vivo or to isolate defined populations of hemocytes for use in experiments in vitro. This is particularly true for vector arthropods like mosquitoes where little information exists on the origins, differentiation or functions of different hemocyte types. Mosquito hemocytes are classified primarily on the basis of morphology with only limited data relating these morphological characters to functional features. In the case of critically important vectors, like Anopheles gambiae, virtually nothing is known about hemocyte ontogeny in any life stage. Here, our goal is to use hemocyte-like cell lines (4a-3B cells), the fully sequenced An. gambiae genome, and our knowledge of hemocyte lineage markers from other insects to develop a robust suite of characters for identifying An. gambiae hemocytes. Given the exploratory nature of the application and limited background available on An. gambiae hemocytes, we think it appropriate to submit the application as an R21 proposal. Specific aims are: 1. Clone and characterize subpopulations of 4a-3B cells, 2. Characterize candidate generic and gene specific markers in An. gambiae hemocytes and 4a-3B cells, and 3. Conduct a proteomic analysis of the subcloned 4a-3B cells to identify new candidate markers. The results of this study will provide essential tools for understanding the role of different hemocytes in cellular immunity of An. gambiae and likely other species of mosquitoes.

描述（由申请人提供）：昆虫免疫系统由细胞和体液元素组成，这些元素天生就会识别出广泛的外国入侵者。尽管在过去几年中取得了巨大进展，以识别抗菌肽等体液因素和调节其合成的信号传导途径，但对控制细胞防御反应的控制知之甚少。这在很大程度上是由于许多昆虫的尺寸很小，这些昆虫使血细胞收集以及血细胞产生的效应分子的鉴定困难。通常也很难在体内对血细胞介导的防御反应进行操纵实验或分离定义的血细胞种群以在体外实验中使用。对于诸如蚊子（例如蚊子）的媒介节肢动物尤其如此，在这些蚊子中，几乎没有有关不同血细胞类型的分化或功能的信息。蚊子血细胞主要基于形态分类，仅数据将这些形态特征与功能特征相关的数据有限。对于非常重要的载体，例如Anopheles Gambiae，在任何生命阶段，几乎对血细胞个体发育的了解一无所知。在这里，我们的目标是使用血细胞样细胞系（4A-3B细胞），完全测序的An。冈比亚基因组以及我们对其他昆虫血细胞谱系标记的了解，以开发出可靠的特征来识别AN。冈比亚血细胞。考虑到应用程序的探索性质和有限的背景。冈比亚血细胞，我们认为将该申请作为R21提案提交是适当的。具体目的是：1。克隆和表征4A-3B细胞的亚群，2。在An中表征候选的通用和基因特异性标记。冈比亚血细胞和4A-3B细胞，以及3。对亚克隆的4A-3B细胞进行蛋白质组学分析，以鉴定新的候选标记。这项研究的结果将为理解不同血细胞在AN的细胞免疫中的作用提供必不可少的工具。冈比亚以及可能的其他蚊子。