Mechanisms of Dorsal Ventral Patterning in Insects

昆虫背腹模式的机制

• 批准号: 7275575
• 负责人: Jeremy A Lynch
• 金额: $ 0.7万
• 依托单位: UNIVERSITY OF COLOGNE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7275575
• 关键词: Coleoptera; Drosophilidae; Hymenoptera; Orthoptera; RNA interference; analog; arthropod genetics; biochemical evolution; biological models; cell differentiation; cellular polarity; cytoskeleton; developmental genetics; egg /ovum; epidermal growth factor; expression cloning; functional /structural genomics; gene induction /repression; growth factor receptors; invertebrate embryology; microarray technology; model design /development; nucleic acid sequence; postdoctoral investigator; species difference; transforming growth factors

DESCRIPTION (provided by applicant): Expanding knowledge of developmental mechanisms beyond traditional model organisms should provide insights into the function of basic biological processes, and how these processes are employed in different environments. The beetle Tribolium is emerging as an important alternative to Drosophila. The focus of this proposal will be on the establishment of the dorsal ventral (DV) axis in Tribolium. The importance of oocyte cytoskeletal organization and function will be examined using antibody staining, transgenic fusion proteins and drug treatments. Then, expression and function of genes involved in sending and receiving signals from the oocyte nucleus will be examined by gene expression patterns and RNA interference. Candidate genes will be identified by homology to participants in the Drosophila DV system, and by sequencing and testing ESTs from Tribolium ovaries. At later stages, microarrays will be used to identify targets of the Tribolium Dorsal protein gradient. Potential targets will be identified as those whose expression levels change significantly when Dorsal function is manipulated. Finally, initial steps to further broaden understanding of DV patterning will be taken using three additional insect species amenable to manipulation in the lab.

描述（由申请人提供）：扩大对传统模型生物体以外的发展机制的知识应提供有关基本生物过程功能以及如何在不同环境中使用这些过程的见解。甲虫贡献是果蝇的重要替代品。该提议的重点将放在戏剧性腹侧腹侧（DV）轴上的建立。将使用抗体染色，转基因融合蛋白和药物处理来检查卵母细胞骨骼组织和功能的重要性。然后，将通过基因表达模式和RNA干扰来检查涉及从卵母细胞核发送和接收信号的基因的表达和功能。候选基因将通过与果蝇DV系统的参与者的同源性以及通过Triborium卵巢进行测序和测试的鉴定。在以后的阶段，微阵列将用于识别背侧蛋白质梯度的靶标。潜在目标将被确定为当操纵背侧功能时表达水平发生显着变化的靶标。最后，将使用可在实验室操纵的三种其他昆虫物种采取进一步扩大对DV构图的初步步骤。