Mechanisms of Dorsal Ventral Patterning in Insects

昆虫背腹模式的机制

基本信息

批准号：
7351837
负责人：
Jeremy A Lynch
金额：
$ 3.9万
依托单位：
UNIVERSITY OF COLOGNE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-01-01 至 2009-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7351837
关键词：
Accounting Affect Animal Model Anterior Antibodies Biological Process Blastoderm Candidate Disease Gene Cell Nucleus Cells Chimeric Proteins Cloning Cytoskeletal Modeling Cytoskeleton Development Developmental Process Dorsal Drosophila genus Drosophila melanogaster Embryo Embryonic Development Environment Epidermal Growth Factor Receptor Event Evolution Expressed Sequence Tags Fellowship Gene Expression Genes Genomics Gryllidae Homologous Gene Immigration Individual Insecta Knowledge Localized Maps Mesoderm Messenger RNA Microfilaments Microtubules Modeling Names Numbers Oocytes Organism Orthologous Gene Ovarian Ovarian Tissue Ovary Participant Pattern Pharmaceutical Preparations Phylogenetic Analysis Positioning Attribute Process Proteins RNA Interference Role Sampling Series Signal Transduction Staging Staining method Stains System Taxon Techniques Testing Transgenic Organisms Tribolium Wasps base computerized data processing dorsal proteins fly gene function genetic analysis insight research study

项目摘要

Expanding knowledge of developmental mechanisms beyond traditional model organisms should provide insights into the function of basic biological processes, and how these processes are employed in different environments. The beetle Tribolium is emerging as an important alternative to Drosophila. The focus of this proposal will be on the establishment of the dorsal ventral (DV) axis in Tribolium. The importance of oocyte cytoskeletal organization and function will be examined using antibody staining, transgenic fusion proteins and drug treatments. Then, expression and function of genes involved in sending and receiving signals from the oocyte nucleus will be examined by gene expression patterns and RNA interference. Candidate genes will be identified by homology to participants in the Drosophila DV system, and by sequencing and testing ESTs from Tribolium ovaries. At later stages, microarrays will be used to identify targets of the Tribolium Dorsal protein gradient. Potential targets will be identified as those whose expression levels change significantly when Dorsal function is manipulated. Finally, initial steps to further broaden understanding of DV patterning will be taken using three additional insects species amenable to manipulation in the lab.

扩大对传统模型生物以外的发展机制的知识应提供洞悉基本生物过程的功能，以及如何在不同的环境。甲虫贡献是果蝇的重要替代品。重点提案将是在戏剧中建立背腹（DV）轴的建议。卵母细胞的重要性将使用抗体染色，转基因融合蛋白检查细胞骨架组织和功能和药物治疗。然后，在发送和接收信号中涉及的基因的表达和功能卵母细胞核将通过基因表达模式和RNA干扰来检查。候选基因将通过同源性与果蝇DV系统的参与者以及测序和测试来识别来自Tribolium卵巢的EST。在以后的阶段，微阵列将用于识别贡献的目标背蛋白梯度。潜在目标将被确定为表达水平改变的目标当操纵背部功能时，显着。最后，最初的步骤进一步扩大对DV的了解将使用其他可以在实验室操纵的其他昆虫物种进行模式。