Mechanisms of Dorsal Ventral Patterning in Insects

昆虫背腹模式的机制

基本信息

批准号：
7351837
负责人：
Jeremy A Lynch
金额：
$ 3.9万
依托单位：
UNIVERSITY OF COLOGNE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-01-01 至 2009-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7351837
关键词：
Accounting Affect Animal Model Anterior Antibodies Biological Process Blastoderm Candidate Disease Gene Cell Nucleus Cells Chimeric Proteins Cloning Cytoskeletal Modeling Cytoskeleton Development Developmental Process Dorsal Drosophila genus Drosophila melanogaster Embryo Embryonic Development Environment Epidermal Growth Factor Receptor Event Evolution Expressed Sequence Tags Fellowship Gene Expression Genes Genomics Gryllidae Homologous Gene Immigration Individual Insecta Knowledge Localized Maps Mesoderm Messenger RNA Microfilaments Microtubules Modeling Names Numbers Oocytes Organism Orthologous Gene Ovarian Ovarian Tissue Ovary Participant Pattern Pharmaceutical Preparations Phylogenetic Analysis Positioning Attribute Process Proteins RNA Interference Role Sampling Series Signal Transduction Staging Staining method Stains System Taxon Techniques Testing Transgenic Organisms Tribolium Wasps base computerized data processing dorsal proteins fly gene function genetic analysis insight research study

项目摘要

Expanding knowledge of developmental mechanisms beyond traditional model organisms should provide insights into the function of basic biological processes, and how these processes are employed in different environments. The beetle Tribolium is emerging as an important alternative to Drosophila. The focus of this proposal will be on the establishment of the dorsal ventral (DV) axis in Tribolium. The importance of oocyte cytoskeletal organization and function will be examined using antibody staining, transgenic fusion proteins and drug treatments. Then, expression and function of genes involved in sending and receiving signals from the oocyte nucleus will be examined by gene expression patterns and RNA interference. Candidate genes will be identified by homology to participants in the Drosophila DV system, and by sequencing and testing ESTs from Tribolium ovaries. At later stages, microarrays will be used to identify targets of the Tribolium Dorsal protein gradient. Potential targets will be identified as those whose expression levels change significantly when Dorsal function is manipulated. Finally, initial steps to further broaden understanding of DV patterning will be taken using three additional insects species amenable to manipulation in the lab.

将发育机制的知识扩展到传统模式生物之外应该提供深入了解基本生物过程的功能，以及这些过程如何应用于不同的领域环境。赤拟虫正在成为果蝇的重要替代品。本次的重点提案将涉及在Tribolium中建立背腹（DV）轴。卵母细胞的重要性将使用抗体染色、转基因融合蛋白检查细胞骨架组织和功能和药物治疗。然后，参与发送和接收信号的基因的表达和功能卵母细胞核将通过基因表达模式和RNA干扰进行检查。候选基因将通过与果蝇 DV 系统中的参与者的同源性以及测序和测试来识别来自谷盗卵巢的 EST。在后期阶段，微阵列将用于识别 Tribolium 的目标背侧蛋白质梯度。潜在目标将被确定为表达水平发生变化的目标当操纵背侧功能时，效果显着。最后，进一步扩大对 DV 理解的初步步骤将使用另外三种易于在实验室操作的昆虫物种进行图案化。