Mechanisms of Dorsal Ventral Patterning in Insects

昆虫背腹模式的机制

• 批准号: 7157366
• 负责人: Jeremy A Lynch
• 金额: $ 3.7万
• 依托单位: UNIVERSITY OF COLOGNE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157366
• 关键词: Coleoptera; Drosophilidae; Hymenoptera; Orthoptera; RNA interference; analog; arthropod genetics; biochemical evolution; biological models; cell differentiation; cellular polarity; cytoskeleton; developmental genetics; egg /ovum; epidermal growth factor; expression cloning; functional /structural genomics; gene induction /repression; growth factor receptors; invertebrate embryology; microarray technology; model design /development; nucleic acid sequence; postdoctoral investigator; species difference; transforming growth factors

DESCRIPTION (provided by applicant): Expanding knowledge of developmental mechanisms beyond traditional model organisms should provide insights into the function of basic biological processes, and how these processes are employed in different environments. The beetle Tribolium is emerging as an important alternative to Drosophila. The focus of this proposal will be on the establishment of the dorsal ventral (DV) axis in Tribolium. The importance of oocyte cytoskeletal organization and function will be examined using antibody staining, transgenic fusion proteins and drug treatments. Then, expression and function of genes involved in sending and receiving signals from the oocyte nucleus will be examined by gene expression patterns and RNA interference. Candidate genes will be identified by homology to participants in the Drosophila DV system, and by sequencing and testing ESTs from Tribolium ovaries. At later stages, microarrays will be used to identify targets of the Tribolium Dorsal protein gradient. Potential targets will be identified as those whose expression levels change significantly when Dorsal function is manipulated. Finally, initial steps to further broaden understanding of DV patterning will be taken using three additional insect species amenable to manipulation in the lab.

描述（由申请人提供）：将发育机制的知识扩展到传统模式生物之外，应该可以深入了解基本生物过程的功能，以及这些过程如何在不同的环境中应用。赤拟虫正在成为果蝇的重要替代品。该提案的重点是在Tribolium中建立背腹（DV）轴。将使用抗体染色、转基因融合蛋白和药物治疗来检查卵母细胞细胞骨架组织和功能的重要性。然后，通过基因表达模式和RNA干扰来检查参与卵母细胞核发送和接收信号的基因的表达和功能。候选基因将通过与果蝇 DV 系统参与者的同源性以及对谷盗卵巢的 EST 进行测序和测试来鉴定。在后期阶段，微阵列将用于识别背谷盗蛋白质梯度的目标。潜在目标将被确定为当背侧功能被操纵时表达水平显着变化的目标。最后，将使用另外三种易于在实验室操作的昆虫物种来进一步扩大对 DV 模式的理解。