The effect of the microbiota on male Aedes aegypti life history traits

微生物群对雄性埃及伊蚊生活史性状的影响

• 批准号: 10574165
• 负责人: Sarah M Short
• 金额: $ 23.63万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-11-22 至 2024-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10574165
• 关键词: Accounting; Adult; Aedes; Affect; Bacteria; Biology; Body Size; Cessation of life; Communities; Complex; Copulation; Culicidae; Data; Dengue Virus; Development; Dietary Supplementation; Disease; Exposure to; Fat Body; Female; Gastrointestinal tract structure; Gland; Gnotobiotic; Goals; Insecta; Insemination; Investigation; La Crosse virus; Laboratories; Length; Location; Longevity; Male Sterility; Male Sterilizations; Measurement; Methods; Microbe; Mosquito Control; Mosquito-borne infectious disease; Nutrient; Organ; Partner in relationship; Pathogenicity; Physiology; Population; Proteins; Quality of life; Reproduction; Reproductive History; Role; Scourge; Seminal fluid; Sexual sterilization; Site; Sterility; Target Populations; Testing; Time; Tissues; Transcript; United States; Water; West Nile; Wing; Work; Zika Virus; disease transmission; fitness; fungus; immune system function; improved; insight; life history; male; microbial; microbiota; novel; novel strategies; offspring; prevent; reproductive; reproductive success; sperm cell; sterile insect technique; success; trait; transcriptome; transmission process; vector control

Project Summary: Mosquitoes transmit multiple diseases worldwide accounting for millions of disease cases and hundreds of thousands of deaths each year. The United States is not immune to the scourge of mosquito-borne disease, as mosquitoes in the USA can transmit dengue and Zika viruses as well as West Nile and La Crosse encephalitis virus. Mosquitoes are also a substantial nuisance that reduce quality of life and enjoyment of outdoor activity. Control of mosquito populations is the primary way we prevent disease transmission and nuisance issues. Many new methods of mosquito population reduction involve a modified version of a method called sterile insect technique (SIT) in which males are made reproductively sterile in a laboratory and released to mate with wild females. This results in zero offspring and a reduction in the population of that targeted mosquito species. SIT requires mass rearing of male mosquitoes in large rearing facilities and these males must be healthy and able to compete with wild males for mates. Here, we propose to investigate the potential impact the mosquito bacterial microbiota (i.e. bacteria in the mosquito body) may have on male Aedes aegypti mosquito traits that are relevant for SIT including longevity, body size, and ability to compete for mates. We will also investigate the way the microbiota affects the transcriptome of males. This work will be the first in-depth investigation into the role of the microbiota in male mosquito fitness traits and will provide key insight into ways the microbiota might be leveraged to improve SIT for mosquito control.

项目摘要： 蚊子在全球范围内传播多种疾病，以计算数百万疾病的病例和 每年成千上万的死亡。美国不能免疫 蚊子传播的疾病，因为美国的蚊子可以将登革热和寨卡病毒作为 以及西尼罗河和拉克罗斯脑炎病毒。蚊子也是一个很大的滋扰 这会降低生活质量和户外活动的享受。蚊子种群的控制是 我们防止疾病传播和滋扰问题的主要方式。许多新方法的 蚊子种群减少涉及一种称为无菌昆虫的方法的修改版本 在实验室中使雄性生殖无菌并释放到的技术（SIT） 与野生女性交配。这导致零后代和人口减少 靶向蚊子。坐骨需要大饲料中的雄性蚊子堆放 设施和这些男性必须健康，并能够与野生男性竞争。这里， 我们建议研究蚊子菌群的潜在影响（即细菌中的细菌 蚊子的身体可能具有与SIT相关的雄性埃及埃及埃及蚊子的特征 包括寿命，身体大小和竞争伴侣的能力。我们还将调查方式 微生物群会影响男性的转录组。这项工作将是第一个深入的 调查微生物群在男性蚊子健康特征中的作用，并将提供关键 洞悉可能利用微生物群以改善蚊子控制的方式。