SPF Breeding Colonies at the Yerkes NPRC

Yerkes NPRC 的 SPF 育种群

• 批准号: 9513118
• 负责人: Joyce Kimberly Cohen
• 金额: $ 90.33万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2018-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9513118
• 关键词: AIDS prevention; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Age; Animals; Breeding; Data; Development; Ensure; Equilibrium; Funding; Generations; Genetic; Genetic Enhancement; Genetic Variation; Genotype; Growth; HIV Infections; Health; Human; Human Resources; Income; Institution; Laboratories; Macaca; Macaca mulatta; Major Histocompatibility Complex; Methodology; Phase; Population; Practice Management; Pre-Clinical Model; Primates; Production; Research; Research Personnel; Resources; SIV; Sales; Site; Support Groups; Testing; Tissues; Type D Retrovirus; United States National Institutes of Health; Universities; Virus; Virus Diseases; age stratification; aged; base; cost; cost effective; experimental study; genetic information; genetic pedigree; germ free condition; improved; juvenile animal; member; nonhuman primate; population stratification; prevent; programs; public health relevance; response; screening; seroconversion; social; virology

DESCRIPTION (provided by applicant): The development of effective strategies to prevent and treat HIV infection is of paramount importance to improve global human health. Simian immunodeficiency virus (SIV) infection of rhesus macaques (Macaca mulatta) is the most effective pre-clinical model of HIV infection. Members of the Macaca genus, however, harbor several viruses that could confound experimental results from studies relating to the AIDS prevention or cure. It is therefore vital to ensure a constant supply of specific pathogen free (SPF) rhesus macaques for experimental studies in AIDS research. Since 2002, the SPF Rhesus macaque breeding groups at Yerkes National Primate Research Center (YNPRC) have, in part, been supported by U24 funding. By the end of 2013, all breeding groups will be free of SIV, Simian T-Lymphotropic Virus (STLV), Simian Type D Retroviruses (SRV), and Herpes Simian B Virus (Herpes-B). The central aim of this final phase of U24 funding is to achieve financial sustainability for those U24-supported groups and to expand multi-generational, genetically characterized breeding groups to provide appropriately aged experimental animals for NIH funded AIDS research. To achieve this aim, proven management practices will be employed that allow optimum production of animals in socially stable breeding groups to provide animals to AIDS-related studies. A second aim of this project is to expand methodology for SRV and Herpes testing in the Yerkes Virology Core. On-site SRV and Herpes B testing will save costs and allow a more rapid colony management response should the virus status of any SPF animal be questionable. A third aim of this renewal is to transition to a more cost-effective, therefore financially sustainable, SNP-based and direct sequencing approach for macaque genotyping, providing AIDS investigators with pedigreed animals with confirmed sets of major histocompatibility complex (MHC). It is expected that the YNPRC SPF rhesus macaque breeding colony will be financially sustainable by the end of the next U24 funding period and will be recognized as a national resource of genetically well-characterized rhesus macaques available for HIV/AIDS research.

描述（由申请人提供）：制定预防和治疗艾滋病毒感染的有效策略对于改善全球人类健康至关重要。恒河猴（Macaca mulatta）的猿猴免疫缺陷病毒（SIV）感染是最有效的 HIV 感染临床前模型。然而，猕猴属成员携带多种病毒，可能会混淆艾滋病预防或治疗相关研究的实验结果。因此，确保持续供应无特定病原体 (SPF) 的恒河猴用于艾滋病研究的实验研究至关重要。自 2002 年以来，耶基斯国家灵长类研究中心 (YNPRC) 的 SPF 恒河猴繁殖小组部分得到了 U24 资金的支持。到2013年底，所有繁殖群体将不再感染SIV、猿T淋巴细胞病毒（STLV）、猿D型逆转录病毒（SRV）和猿B型疱疹病毒（Herpes-B）。 U24 资助最后阶段的中心目标是实现 U24 支持的团体的财务可持续性，并扩大多代、遗传特征育种团体，为 NIH 资助的艾滋病研究提供适当年龄的实验动物。为了实现这一目标，将采用经过验证的管理实践，使社会稳定的繁殖群体能够实现动物的最佳生产，从而为艾滋病相关研究提供动物。该项目的第二个目标是扩展 Yerkes Virology Core 中 SRV 和疱疹检测的方法。现场 SRV 和 B 型疱疹检测将节省成本，并在任何 SPF 动物的病毒状况有疑问时可以更快地做出群体管理反应。此次更新的第三个目标是过渡到一种更具成本效益、因此经济上可持续、基于 SNP 的猕猴基因分型直接测序方法，为艾滋病研究人员提供具有已确认的主要组织相容性复合体 (MHC) 组的纯系动物。预计 YNPRC SPF 恒河猴繁殖群将在下一个 U24 资助期结束时实现财务可持续，并将被视为可用于艾滋病毒/艾滋病研究的遗传特征良好的恒河猴国家资源。