Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center

耶基斯国家灵长类研究中心 SPF 繁殖群的维护

基本信息

批准号：
10203159
负责人：
Joyce Kimberly Cohen
金额：
$ 79.98万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-30 至 2022-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10203159
关键词：
2019-nCoV AIDS/HIV problem Acquired Immunodeficiency Syndrome Alleles Animal Model Animals Biological Assay Breeding COVID-19 Code Development Diagnostic tests Disease Disease Progression Ensure Enzyme-Linked Immunosorbent Assay Fees Female Frequencies Funding Genes Genetic Genetic Screening Genomic DNA Genotype Grant Growth HIV HIV Infections Health Immunity Income Infection Leadership MHC Class I Genes Macaca mulatta Maintenance Methods Modeling Molecular Nose Pathogen detection Pathogenicity Pattern Primates Process Production Request for Applications Research Research Project Grants Resources Sampling Scientist Serological Serum Swab Techniques Testing Text Messaging United States National Institutes of Health Vaccines Variant Viral Virus Virus Diseases Work X Chromosome aged animal colony genetic information genetic pedigree genome-wide germ free condition insight male nonhuman primate preclinical study prevent programs receptor risk minimization screening therapy development viral detection working group

项目摘要

The AIDS research portfolio at Yerkes National Primate Research Center (YNPRC) has continued to expand as demonstrated by a growth of approximately $12 million dollars (57% increase) in nonhuman primate (NHP) AIDS research grant funding over the past five years. This growth in grant funding is accompanied by a high demand for Indian-origin specific pathogen free (SPF) rhesus macaques as the animal model for this research. YNPRC has maintained a colony of SPF rhesus macaques (previously with U24 support) in order to provide these animals for HIV/AIDS research. This application requests continued support (now a U42 mechanism) to expand the SPF colony by maximizing production. The application includes the Overview; Husbandry and Management Core; the Viral Testing Core; and the MHC Genetic Typing Core. This grant will support a subset of the overall YNPRC SPF colony that derive program income from animal assignment fees and per diems. YNPRC will provide institutional support to cover remaining expenses for this colony as well as the remainder of the SPF colony. Management and Husbandry: To leverage the resources provided by the U42 to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. Viral Testing: To provide serological and molecular viral diagnostic testing in support of the SPF colony and to maintain a colony free of diseases that impact AIDS-related research at the Yerkes NPRC. In this supplement we propose to develop and implement molecular and serologic methods for detecting SARS-CoV-2 infection and immunity in the SPF rhesus macaque colony at Yerkes National Primate Research Center. MHC Genetic Typing: To provide parentage testing, comprehensive analysis of MHC class I and class II alleles, and to develop new techniques for detailed genetic characterization to support a genetically healthy breeding colony and provide genetic information on animals essential to the animal assignment process in support of AIDS research. In this supplement we propose to define and determine the frequency of distinct ACE2 coding alleles within the YNPRC rhesus macaque breeding colony. Achieving these aims will ensure the Yerkes NPRC SPF breeding program is healthy, providing the necessary animals to support our HIV/AIDS research program using Indian-origin rhesus monkeys. The sustainability of this critical resource to scientists performing preclinical studies using the rhesus monkey model will have a positive, significant impact on the development of treatments to prevent or cure infection from HIV in people.

耶基斯国家灵长类研究中心 (YNPRC) 的艾滋病研究组合不断扩大非人类灵长类动物 (NHP) 的增长约 1200 万美元（增长 57%）证明了这一点过去五年的艾滋病研究拨款。赠款资金的增长伴随着高需要印度原产的无特定病原体 (SPF) 恒河猴作为本研究的动物模型。 YNPRC 维持了一个 SPF 恒河猴群（之前有 U24 支持），以便提供这些动物用于艾滋病毒/艾滋病研究。此应用程序请求继续支持（现在是 U42 机制）通过最大化生产来扩大 SPF 菌落。该应用程序包括概述；畜牧业和管理核心；病毒检测核心；和 MHC 基因分型核心。这笔赠款将支持一个子集整个 YNPRC SPF 群体的项目收入来自动物分配费和每日津贴。 YNPRC 将提供机构支持，以支付该殖民地以及其他地区的剩余费用 SPF 菌落。管理和饲养：利用 U42 提供的资源最大限度地提高产量印度原产恒河猴，并促进将这些动物分配给美国国立卫生研究院资助的艾滋病毒/艾滋病研究。病毒检测：提供血清学和分子病毒诊断检测以支持 SPF 群体并维持 Yerkes NPRC 免受影响艾滋病相关研究的疾病的影响。在这个补充我们建议开发和实施分子和血清学方法来检测耶基斯国家灵长类动物 SPF 恒河猴群体中的 SARS-CoV-2 感染和免疫研究中心。 MHC基因分型：提供亲子鉴定，MHC I类和II类综合分析等位基因，并开发详细遗传特征的新技术，以支持遗传健康繁殖群体并提供对动物分配过程至关重要的动物遗传信息支持艾滋病研究。在本补充中，我们建议定义和确定频率 YNPRC 恒河猴繁殖群体中存在不同的 ACE2 编码等位基因。实现这些目标将确保 Yerkes NPRC SPF 育种计划的健康发展，提供必要的条件使用印度恒河猴来支持我们的艾滋病毒/艾滋病研究计划。的可持续性对于使用恒河猴模型进行临床前研究的科学家来说，这一重要资源将具有对预防或治愈人类艾滋病毒感染的治疗方法的开发产生积极、重大的影响。