CORE B - ANIMAL CORE

核心 B - 动物核心

基本信息

批准号：
8248586
负责人：
Francesco John DeMayo
金额：
$ 14.05万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

项目摘要

The Animal Core will serve as a resource to supply Center investigators with the necessary animals and animal research techniques to effectively execute their research. The Animal Core will be responsible for the purchasing and inventory of the animals used by Center investigators, as well as, providing investigators with the technical ability required in the manipulation of the various animal models. In the past the Animal Core was responsible for the generation of genetically engineered mice. However, the focus of the research in this center application will be on investigation of endometrial biology in mouse models already generated. Therefore, the emphasis of this Core in the renewal will be to serve as a repository for the animals used in the core and for the phenotypic analysis of impact of the genetic alterations on mouse reproductive biology. This Core will incorporate the use of xenotransplants of human endometrial tissue into NOD/SCID/yc nu" (NOG) mice, as well as, the use of mouse models for endometriosis. The Core will be responsible for the execution of these complicated techniques for the analysis of endometrial biology and will only generate new models as deemed necessary. The Core will serve Center Investigators by accomplishing the following tasks: 1. The Animal Core will provide the phenotypic analysis of the impact of specific genetic alterations on mouse reproductive biology. 2. The Animal Core will conduct xenograft procedures involved in the reconstitution of endometrial tissue into mice. 3. The Animal Core will manage the colonies of genetically engineered mice for Center Investigators. 4. The Animal Core will produce mutant mice by microinjecting genetically engineered Embryonic Stem cells into the blastocoel of the embryonic day 3.5 mouse embryos and transgenic mice by DNA microinjection into the male pronucleus of one-cell mouse embryos.

动物核心将作为向中心调查人员提供必要动物的资源，动物研究技术有效地执行他们的研究。动物核心将负责中心调查人员使用的动物的采购和清单，并为调查人员提供操纵各种动物模型所需的技术能力。过去，动物核心是负责产生基因工程小鼠。但是，该研究中心的重点应用将在已经生成的小鼠模型中研究子宫内膜生物学。因此，续签中该核心的重点将是作为核心和核心用动物的存储库遗传改变对小鼠生殖生物学的影响的表型分析。这个核心将将人子宫内膜组织的异种移植物纳入点头/scID/yc nu”（nog）小鼠 AS，将小鼠模型用于子宫内膜异位症。核心将负责执行这些用于分析子宫内膜生物学的复杂技术，只会产生新模型必要的。核心将通过完成以下任务来为中心调查人员提供服务： 1。动物核心将提供对特定遗传改变小鼠影响的表型分析生殖生物学。 2。动物核心将进行涉及子宫内膜组织重构的异种移植程序老鼠。 3。动物核心将为中心研究人员管理基因工程小鼠的菌落。 4。动物核将通过微注射基因工程的胚胎干细胞产生突变小鼠通过DNA微注射到一细胞小鼠胚胎的雄性原核。