Genetically Engineered Mouse Resource

基因工程小鼠资源

基本信息

批准号：
7514629
负责人：
Francesco John DeMayo
金额：
$ 8.39万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-01 至 2010-06-30
项目状态：
已结题

项目摘要

The objective of the Genetically Engineered Mouse (GEM) Resource is to aid Cancer Center investigators with the design and execution of experiments to utilize animal models for the investigation of the progression of cancer and for the preclinical evaluation of therapies for the treatment of cancer. Although the use of animal models provides an invaluable source of reagents for preclinical testing and the investigation of regulatory mechanisms in vivo in a physiologically regulated system, the use of animals is costly with respect to equipment, animal resources, and technical expertise. The GEM Core will serve as a resource for carrying out and training in the use of all animal manipulations needed in the Cancer Center. The GEM Core will minimize the cost of utilizing animal models in the Cancer Center by consolidating the equipment, expertise, and animal resources. This will ensure an efficient use of animal research at minimal cost. The GEM Core will serve Cancer Center investigators by aiding in the following tasks. 1. The GEM Core will generate transgenic mice by the micro injection of DMA into one-celled mouse embryos. 2. The GEM Core will generate mutant mice by the manipulation of mouse Embryonic Stem Cells. 3. The Core will preserve lines of mice by cryopreservation. 4 The Core will aid investigators with the importation and exporting of lines of mice. The accomplishments of these tasks will allow the GEM Core to serve as a repository for all genetically engineered mouse models and techniques for Cancer Center investigators and will ensure efficient use of animal resources by Cancer Center investigators. The Shared Resource Leader is Dr. Francesco DeMayo, who has an extensive track record in these areas

基因工程小鼠（GEM）资源的目的是帮助癌症中心研究人员随着实验的设计和执行，利用动物模型来研究进展癌症和临床前评估癌症治疗的疗法。虽然使用动物模型为临床前测试和研究提供了宝贵的试剂来源在生理调节系统中体内的调节机制，动物的使用成本高昂到设备，动物资源和技术专长。宝石核心将作为在癌症中心进行所有需要的动物操纵，进行和培训。宝石核心通过巩固设备，将在癌症中心利用动物模型的成本最小化，专业知识和动物资源。这将确保有效利用动物研究的成本最低。宝石核心将通过协助以下任务来为癌症中心的研究人员提供服务。 1。宝石芯将通过将DMA的微型注射到单细胞小鼠中产生转基因小鼠胚胎。 2。宝石核将通过操纵小鼠胚胎干细胞产生突变小鼠。 3。核心将通过冷冻保存来保留小鼠线。 4核心将帮助调查人员进口和出口小鼠。这些任务的成就将使宝石核心可以作为所有基因的存储库为癌症中心研究人员设计的鼠标模型和技术，将确保有效利用癌症中心研究人员的动物资源。共享资源负责人是Francesco DeMayo博士，谁在这些领域有广泛的记录