CORE B - ANIMAL CORE

核心 B - 动物核心

基本信息

批准号：
7683522
负责人：
Francesco John DeMayo
金额：
$ 11.52万
依托单位：
BAYLOR COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

项目摘要

The Animal Core will serve as a resource to supply Center investigators with the necessary animals and animal research techniques to effectively execute their research. The Animal Core will be responsible for the purchasing and inventory of the animals used by Center investigators, as well as, providing investigators with the technical ability required in the manipulation of the various animal models. In the past the Animal Core was responsible for the generation of genetically engineered mice. However, the focus of the research in this center application will be on investigation of endometrial biology in mouse models already generated. Therefore, the emphasis of this Core in the renewal will be to serve as a repository for the animals used in the core and for the phenotypic analysis of impact of the genetic alterations on mouse reproductive biology. This Core will incorporate the use of xenotransplants of human endometrial tissue into NOD/SCID/yc nu" (NOG) mice, as well as, the use of mouse models for endometriosis. The Core will be responsible for the execution of these complicated techniques for the analysis of endometrial biology and will only generate new models as deemed necessary. The Core will serve Center Investigators by accomplishing the following tasks: 1. The Animal Core will provide the phenotypic analysis of the impact of specific genetic alterations on mouse reproductive biology. 2. The Animal Core will conduct xenograft procedures involved in the reconstitution of endometrial tissue into mice. 3. The Animal Core will manage the colonies of genetically engineered mice for Center Investigators. 4. The Animal Core will produce mutant mice by microinjecting genetically engineered Embryonic Stem cells into the blastocoel of the embryonic day 3.5 mouse embryos and transgenic mice by DNA microinjection into the male pronucleus of one-cell mouse embryos.

动物核心将作为一种资源，为中心调查人员提供必要的动物和有效执行研究的动物研究技术。动物核心将负责中心调查人员使用的动物的采购和库存，以及为调查人员提供操作各种动物模型所需的技术能力。过去动物核心是负责基因工程小鼠的产生。然而，该中心的研究重点应用将用于在已生成的小鼠模型中研究子宫内膜生物学。因此，该核心在更新中的重点将是作为核心中使用的动物的储存库，并用于遗传改变对小鼠生殖生物学影响的表型分析。该核心将将人类子宫内膜组织异种移植的使用纳入 NOD/SCID/yc nu” (NOG) 小鼠，以及例如，使用小鼠模型治疗子宫内膜异位症。核心将负责执行这些用于分析子宫内膜生物学的复杂技术，并且只会产生被认为的新模型必要的。核心将通过完成以下任务为中心研究人员提供服务： 1. 动物核心将提供特定基因改变对小鼠影响的表型分析生殖生物学。 2. 动物核心将进行异种移植手术，将子宫内膜组织重建为老鼠。 3. 动物核心将为中心研究人员管理基因工程小鼠群体。 4. Animal Core将通过显微注射基因工程胚胎干细胞来生产突变小鼠通过DNA显微注射进入胚胎第3.5天小鼠胚胎和转基因小鼠的囊胚腔单细胞小鼠胚胎的雄性原核。