COBRE: LSC HSC: HISTOLOGY CORE

COBRE：LSC HSC：组织学核心

基本信息

批准号：
7610599
负责人：
JACK P STRONG
金额：
$ 9.06万
依托单位：
LSU HEALTH SCIENCES CENTER
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-07-01 至 2008-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7610599
关键词：
Animal Model Animals Cardiac Cardiovascular Pathology Cells Cessation of life Computer Retrieval of Information on Scientific Projects Database Core Facility Cultured Cells Doctor of Medicine Doctor of Philosophy Equipment Evaluation Fixatives Freezing Funding Grant Hematoxylin and Eosin Staining Method Histology Human Human Resources In Situ Hybridization In Situ Nick-End Labeling Injury Institution Methylene blue Microscopic Microtome - medical device Molecular Morphology Mus Myocardium Organ Paraffin Paraffin Embedding Pathology Postdoctoral Fellow Rattus Research Research Personnel Resources Sampling Services Smooth Muscle Actin Staining Method Smooth Muscle Myocytes Source Specimen Staining method Stains T-Lymphocyte Technology Tissue Stains Tissues Training Tunica Intima United States National Institutes of Health base cell injury cryostat experience finesse immunocytochemistry kidney vascular structure macrophage tissue processing tungsten carbide

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Jack Strong, Ph.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, through the efforts of Drs. Strong, Carmen Espinoza, and Dana Troxclair as well as Research Associate Penelope Strenge provide all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目及研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。组织学核心杰克·斯特朗博士组织学核心由医学博士 Jack P. Strong 指导，他是一位在组织学和心血管病理学各个方面拥有丰富经验的研究人员。该组织学核心负责组织处理和染色、动物模型和细胞培养物的病理学分析，以及组织和细胞样品的原位杂交和免疫细胞化学。所有初级研究人员都使用该核心进行细胞和组织分析。组织切片来自固定剂灌注的动物或速冻器官。我们还通过访问 LSUHSC 形态学核心设施中的其他资源来扩展此资源。该核心中的组织处理设备包括 Hypercenter XP 处理器； Histocentre 石蜡包埋站；精细石蜡切片机； Leica CM3050 S 低温恒温器，配备用于整个小鼠切片的碳化钨刀和 EMS 振荡组织切片机。组织学核心提供以下服务： 1、心脏、肾、血管组织处理；对组织进行苏木精、伊红和亚甲蓝染色。 2. 大鼠对照和受损动脉组织（平滑肌肌动蛋白和 Erk-1）的免疫组织化学染色。 3. 使用平滑肌肌动蛋白评估损伤与对照大鼠动脉组织以及对血管内膜平滑肌细胞的影响 4. 基于免疫组织化学的损伤与对照动脉组织中 T 细胞和巨噬细胞的评估 5. TUNEL染色分析心肌细胞损伤和死亡。核心实验室，通过博士的努力。 Strong、Carmen Espinoza、Dana Troxclair 以及研究员 Penelope Strenge 提供了所有适当的技术和经过专门培训的人员，以在显微镜和分子水平上评估来自各种固定的人类和鼠动脉标本以及不同基质上的细胞培养物的这些标本。这一核心设施的使用将在来年继续增长。