COBRE: LSC HSC: HISTOLOGY CORE

COBRE：LSC HSC：组织学核心

• 批准号: 7382069
• 负责人: JACK P STRONG
• 金额: $ 26.78万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382069
• 关键词: COBRE; LSC; HSC; HISTOLOGY; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Histology Core Director ¿ Jack Strong, M.D. The Histology Core is under the direction of Jack P. Strong, M.D., an investigator who has extensive experience in all aspects of histology and cardiovascular pathologies. This Histology Core is responsible for tissue processing and staining, pathology analysis in animal models as well as cell cultures, and in situ hybridization and immunocytochemistry in tissue and cell samples. This core is used by all junior investigators for cell and tissue analysis. Tissue sections are generated from either fixative-perfused animals or from snap-frozen organs. We have also expanded this resource by accessing additional resources in the LSUHSC morphology core facility. Tissue processing equipment in this core includes a Hypercenter XP processor; Histocentre paraffin embedding station; Finesse paraffin microtome; a Leica CM3050 S cryostat equipped with a tungsten carbide knife for whole mouse sectioning and an EMS oscillating tissue slicer. The histology core rendered the following services: 1. Cardiac, renal and vascular tissue processing; Hematoxylin and eosin and methylene blue staining of tissues . 2. Immunohistochemical staining of rat control and damaged arterial tissues (smooth muscle actin and Erk-1). 3. Evaluation of injury-versus-control rat arterial tissues and the effects exerted upon smooth muscle cells in vascular intima using smooth muscle actin 4. Immunohistochemical-based assessments of T-cells and macrophages in injury-versus-control arterial tissues 5. Analysis of cell damage and death in myocardium by TUNEL stains. The core lab, via the efforts of Drs. Strong and Huber, provides all of the appropriate technology and specially trained personnel to evaluate these specimens on a microscopic and molecular level from various fixed human and murine arterial specimens and cell cultures on different matrices. The use of this core facility will continue to grow in the upcoming year.

该主题项目是利用NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。组织学核心主管�JackStrong，M.D。组织学核心位于调查员Jack P. Strong，M.D。的指导下，他在组织学和心血管病理方面的各个方面都有丰富的经验。该组织学核心负责组织加工和染色，动物模型中的病理分析以及细胞培养物，以及在组织和细胞样品中的原位杂交和免疫细胞化学。所有初级研究者都将该核心用于细胞和组织分析。组织切片是由固定体动物或frozen器官产生的。我们还通过访问LSUHSC形态核心设施中的其他资源来扩展此资源。该核心中的组织加工设备包括HyperCenter XP处理器； Histocentre石蜡嵌入站；精细的石蜡微落；配备有钨碳化合金针织物的液体CM3050 S低温恒温器，用于整个小鼠截面和EMS振荡组织切片机。组织学核心提供以下服务：1。心脏，肾脏和血管组织加工；组织的苏木精，曙红和亚甲基蓝色染色。 2。大鼠对照和动脉组织受损（平滑肌肌动蛋白和ERK-1）的免疫组织化学染色。 3。使用平滑肌肌动蛋白4的损伤对照大鼠动脉组织评估血管内膜中平滑肌细胞的作用4。基于免疫组织化学的评估对T细胞和巨噬细胞中损伤的巨噬细胞中的T细胞和巨噬细胞的评估5。分析cell骨损伤和死亡的孔孔和死亡。分析孔层损伤和死亡。核心实验室，通过博士的努力。 Strong and Huber提供了所有适当的技术和经过特殊训练的人，以从不同物质上的各种固定人类和鼠类和鼠类伪像以及细胞培养物评估这些规格，以评估这些规格。在接下来的一年中，该核心设施的使用将继续增长。