Animal Models and Histology Core

动物模型和组织学核心

• 批准号: 10715404
• 负责人: Karen Y Stokes
• 金额: $ 42.64万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10715404
• 关键词: Address; Animal Model; Animals; Area; Awareness; Biology; Blood Pressure; Blood Vessels; Blood flow; Blood gas; Breeding; Calorimetry; Cardiac; Cardiovascular Diseases; Cardiovascular system; Cause of Death; Centers of Research Excellence; Charge; Chemistry; Clinical Chemistry; Collaborations; Complement; Death Rate; Development; Disease model; Equipment; Exercise; Faculty; Feedback; Frequencies; Funding; Funding Agency; Future; Future Teacher; Generations; Genotype; Goals; Grant; Growth; Heart; Histologic; Histology; Human Resources; Immunohistochemistry; Implant; Income; Infrastructure; Institution; Intervention; Lipids; Louisiana; Measurement; Measures; Mentors; Metabolic; Modality; Modeling; Modification; Mus; Operative Surgical Procedures; Organ; Oxidation-Reduction; Pathologic; Pathology; Pathway interactions; Phase; Phenotype; Pilot Projects; Positioning Attribute; Post-Translational Protein Processing; Postdoctoral Fellow; Preparation; Price; Protein Structure Initiative; Rattus; Regulation; Research; Research Personnel; Rodent; Rodent Model; Role; Running; Schools; Science; Services; Signal Transduction; Source; Stains; Study models; Surgical Models; Surveys; System; Techniques; Technology; Telemetry; Temperature; Testing; Time; Tissues; Training; United States; United States National Institutes of Health; VO2max; Wages; cost; exercise regimen; experimental study; heart function; implantation; improved; in vivo Model; instrument; laboratory experience; member; non-invasive imaging; pressure; programs; skills; tissue processing; treadmill; ultrasound

PROJECT ABSTRACT The goal of the Center for Redox Biology and Cardiovascular Disease is to improve the infrastructure for junior and senior investigators and to provide mentoring for the junior faculty members so that they become more competitive for independent NIH funding. The Animal Models & Histology Core B, led by Dr Karen Stokes, provides services to both the COBRE members and others at our institute, with 28 users thus far in Phase 1. Core services are also available to nearby institutes at which our cardiovascular researchers have collaborations. A needs survey of faculty identified some key new equipment/services that faculty require for growth of their research programs, with an anticipated increase to 32 users in Phase 2. Dr Hugh Price will continue to lead the Histology and Genotyping Subcore. This will build upon current existing services that provide tissue processing, cardiovascular-related stains, and a genotyping service, to add an immunohistochemistry service (with an automated stainer to help maintain robust histology and genotyping services). The Cardiovascular Phenotyping Subcore, led by Dr Shenu Bhuiyan, will continue to provide capabilities to characterize the disease models. Current equipment includes a Peri-med PSI system and Periflux 5000 to non-invasively image and measure blood flow, a VisualSonics Vevo 3100 Ultra High Frequency Ultrasound to study cardiac and vascular function, an AD Instruments MPVS-Ultra single segment Pressure-Volume Unit for in depth cardiac function measurements, two TSE telemetry systems for continuous recording of blood pressure, activity, temperature, and biopotentials, and an OxyletPro System with a single lane mouse calorimetry treadmill for VO2 max determination during forced exercise. In Phase 2, the telemetry and OxyletPro systems will be complemented by the addition of a CODA High Throughput Noninvasive blood pressure system, for low-cost simultaneous blood pressure measurements in 8 mice/rats, and a 5-lane treadmill for higher throughput exercise regimens respectively, and a blood gas/basic clinical chemistry analyzer will be added. This subcore will also facilitate more in depth clinical chemistry analysis (e.g. organ specific or lipid panels) by external sources. Full-time research technicians have been well-trained on all current modalities and will be trained on future services, so that they can continue to either provide the services, or train lab personnel to perform their experiments using the available technologies. The CCDS Surgical Core will continue to liaise with the CoBRE Core B to implant telemetry probes, and to set up surgical rodent models that undergo subsequent phenotyping in Core B. Taken together, the services offered by the Animal Models and Histology Core will help PIs generate animal models suitable for their research questions, and provide cutting edge phenotypic and pathological analysis to maximize the competitiveness of our junior faculty for future funding in the area of redox regulation of cardiovascular disease.

项目摘要 氧化还原生物学和心血管疾病中心的目标是改善初级基础设施 和高级调查人员，并为初级教师提供指导，以便他们变得更多 具有独立NIH资金的竞争力。动物模型和组织学核心B，由Karen Stokes博士领导， 为我们的研究所的Cobre成员和其他人提供服务，到目前为止，有28位用户在第1阶段。 我们的心血管研究人员合作的附近研究所也可以使用核心服务。 对教师的需求调查确定了教师需要增长的一些关键新设备/服务 研究计划，预计在第2阶段中将增加32位用户。休·普莱斯（Hugh Price）将继续领导 组织学和基因分型子核心。这将建立在当前提供组织处理的现有服务的基础上 与心血管相关的污渍和基因分型服务，以增加免疫组织化学服务（带有 自动化污渍，以帮助维持强大的组织学和基因分型服务）。心血管表型 由Shenu Bhuiyan博士领导的子核心将继续提供表征疾病模型的能力。 当前的设备包括一个毛骨状的PSI系统和periflux 5000到非侵入性图像和测量 血流，Visualsics Vevo 3100超高频超声检查心脏和血管功能， AD Instruments MPVS-Ultra单节压力量单元，用于深度心脏功能 测量，两个TSE遥测系统，用于连续记录血压，活动，温度， 和生物电位，以及一个带有单车道的小鼠量热量跑步机的OxyletPro系统 强迫运动中的决心。在第2阶段，遥测和OxyletPro系统将得到补充 通过添加尾声高吞吐量无创血压系统，同时血液同时血液 8只小鼠/大鼠的压力测量和一个5车道跑步机，用于更高的吞吐量运动方案 将分别添加血液/基本临床化学分析仪。该子核心也将有助于 外部来源的更多深度临床化学分析（例如器官特异性或脂质面板）。全日制 研究技术人员在所有当前方式都受过良好的培训，并将接受未来服务的培训，因此 他们可以继续提供服务，或者培训实验室人员使用 可用技术。 CCDS手术核心将继续与毛核B植入植入物 遥测探针，并建立手术啮齿动物模型，以随后的表型在核心B中。 动物模型和组织学核心提供的服务将共同帮助PIS产生动物模型 适合其研究问题，并提供最先进的表型和病理分析以最大化 我们的初级教师在心血管氧化还原监管领域的未来资金竞争力 疾病。