CONVERSION OF FRETR CATEGORIES TO DISTANCES

将 FRTR 类别转换为距离

• 批准号: 7602196
• 负责人: ERIC MULLER
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602196
• 关键词: Categories; Class; Computer Retrieval of Information on Scientific Projects Database; Cyclophosphamide/Fluorouracil/Prednisone; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Grant; Image; Institution; Measures; Metric; Modeling; Property; Relative (related person); Research; Research Personnel; Resources; Source; United States National Institutes of Health; fluorophore; spindle pole body

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The FRETR metric was developed to measure FRET between CFP and YFP. It replaces other metrics which generally are nonlinear and are insensitive to low values. FRETR is relative, empirical measure of FRET. It expresses the fold increase of fluorescence from FRET over a baseline derived from the inherent fluorescent properties of CFP and YFP. In our study on the organization of the spindle pole body (SPB), FRETR values were divided into 5 broad categories: none, lowest, low, moderate and high. Our model of the SPB and cryo-EM images of the SBP put limits on the possible distances between CFP and YFP for each class. This project will further explore the relationship between FRETR values and the distance between fluorophores.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 开发了FRETR指标以测量CFP和YFP之间的fret。它取代了通常非线性并且对低值不敏感的其他指标。 FRETR是相对的，fret的经验度量。 它表达了从CFP和YFP固有的荧光性能得出的基线上的荧光倍数增加。 在我们对主轴杆体（SPB）组织的研究中，FRERT值分为5个广泛类别：无，最低，低，中和高。我们的SPB和Cryo-EM图像的SBP模型在每个类别的CFP和YFP之间的距离上限制了限制。该项目将进一步探索Fretr值与荧光团之间的距离之间的关系。