IBR-COBRE Phase III

IBR-COBRE III期

基本信息

批准号：
10726483
负责人：
WILLIAM S WARD
金额：
$ 29.1万
依托单位：
UNIVERSITY OF HAWAII AT MANOA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-13 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10726483
关键词：
Adopted Affect Air Appearance Area Basal Plate Biological Specimen Banks Blood Calcium Caucasians Centers of Research Excellence Characteristics Chorionic villi Circulation Classification Clothing Collection Communities Cooking and Eating Utensils Cosmetics Deposition Detection Development East Asian Ecosystem Electron Probe Microanalysis Environment Environmental Pollution Equipment Evaluation Exposure to Fetal Growth Retardation Fibrosis Filipino Foreign-Body Reaction Functional disorder Furniture Goals Hawaii Health Hematoxylin and Eosin Staining Method Hospital Supply Human Human Milk Hypertension Image Infrared Spectrophotometry Ingestion Inhalation International Laboratory Research Life Link Literature Lung Methodology Modernization Native Hawaiian Nodule Pacific Islands Pacific Ocean Palpable Palpation Pathologist Phase Physiology Placenta Plastics Play Pollution Pre-Eclampsia Pregnancy Pregnancy Complications Pregnant Women Premature Birth Prenatal Diagnosis Production Protocols documentation Raman Spectrum Analysis Reporting Role Sampling Scanning Electron Microscopy Shipping Spectroscopy, Fourier Transform Infrared Stains Time Tissues Umbilical Cord Blood Umbilical cord structure Universities Water Woman Women&apos s Health adverse pregnancy outcome biobank calcification cohort exposed human population fetal fluorescence lifetime imaging human tissue interest marine microscopic imaging ocean currents packaging material particle placental membrane prospective recruit reproductive ultrasound wasting

项目摘要

Plastics are found in many aspects of modern life due to their physical characteristics and convenience. They are used every day for disposable shipping and packaging materials, cosmetics, cooking and eating utensils, plastic water bottles, hospital supplies, furniture and clothing, and even laboratory research equipment. Global plastic production has increased from an annual production of 1.5 million metric tons in 1950 to 368 million metric tons in 2018. Subsequent environmental pollution with commercial plastics is rapidly becoming an international problem that is affecting human health and the health of our marine ecosystem. The State of Hawai’i is a land mass located in the middle of the Pacific Ocean that is surrounded by ocean currents that wash large amounts of circulating plastic waste upon our beaches. Over time, plastic debris degrades into microplastics which can be inhaled in the air by humans or ingested by marine life in the waters. Alarmingly, pieces of microplastics have recently been isolated in isolated in human tissue, such as the gut and placenta. The isolation of physical pieces of microplastic particles from human placenta was recently reported in the literature, but the effects that the actual plastic compounds play on maternal, fetal, and placental physiology is poorly understood. As the burden of human exposure to microplastics grows, so does the need to elucidate the role that microplastics may play in affecting pregnancy and women’s health. We will use the Hawaii Reproductive Biospecimen Repository’s (HRBR) collection of banked placenta samples from the past 2 decades to explore the quantity and quality of microplastics found in Hawai’i. It is well known that there are higher rates of preeclampsia and other hypertensive disorders in women with Native Hawaiian and Other Pacific Island (NHOPI) and Filipino heritage compared to Caucasian women. We hypothesize that the presence of microplastics in placental tissue will parallel the increase in plastics pollution in our environment and may play a role placental function and the pathophysiology of adverse pregnancy outcomes such as preeclampsia and fetal growth restriction. We aim to characterize and quantify the presence of microplastics in banked placentas from complicated and uncomplicated pregnancies, leveraging the well-characterized biorepository of collected placenta samples from 2006-2013 using Micro-Raman Spectroscopy and other modern methodologies. In addition, a new cohort of fresh placentas from 2022-2023 will be prospectively collected to explore whether (i) MPs can cross the placental barrier into the fetal umbilical cord and umbilical cord blood and (ii) investigate if placental calcification is linked to accumulating MPs and histopathologic changes associated with preeclampsia and fetal growth restriction, including fibrosis, microinfarctions, and chorangiosis.

由于其身体特征和便利性，在现代生活的许多方面都可以找到塑料。他们每天都用于处置运输和包装材料，化妆品，烹饪和餐具，塑料水瓶，医院用品，家具和衣服，甚至实验室研究设备。全球的塑料产量从1950年的150万吨产量增加到3.68亿公吨 2018年的吨。随后的商业塑料的环境污染正在迅速成为国际影响人类健康和海洋生态系统健康的问题。夏威夷国是一块土地质量位于太平洋中部，周围环流大量洋流在我们的海滩上循环塑料废物。随着时间的流逝，塑料碎片会降解成微塑料，可以被人类参与空中或因水域的海洋生物而摄取。令人震惊的是，微塑料有最近在人体组织中分离出来，例如肠道和斑点。最近报道文献，但实际塑料化合物对母乳，胎儿和胎盘生理学的影响是理解不佳。随着人类暴露于微塑料生长的伯恩（Burnen），阐明的需求也是如此微塑料可能在影响妊娠和妇女健康方面发挥的作用。我们将使用夏威夷生殖 Biospecimen存储库（HRBR）在过去20年中收集了存放的plapeta样品，以探索在夏威夷发现的微塑料的数量和质量。众所周知，有更高的速度夏威夷原住民和其他太平洋岛（NHOPI）的妇女的先兆子痫和其他高血压疾病（NHOPI）和菲律宾遗产与高加索妇女相比。我们假设在胎盘组织将平行于我们环境中塑料污染的增加，并可能扮演胎盘不良妊娠结局的功能和病理生理，例如先兆子痫和胎儿生长限制。我们旨在表征和量化复杂和复杂和简单的怀孕，利用了从 2006-2013使用微拉曼光谱和其他现代方法。此外，一个新的队列预计将收集2022 - 2023年的新鲜餐垫，以探索（i）MPS是否可以越过安置进入胎儿脐带和脐带血的障碍，（ii）调查是否连接了斑点钙化积累MPS和与先兆子痫和胎儿生长限制相关的组织病理学变化，包括纤维化，微吸收和脉络膜作用。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genetically Engineered Pigs as Efficient Salivary Gland Bioreactors for Production of Therapeutically Valuable Human Nerve Growth Factor.

DOI：
10.3390/cells11152378
发表时间：
2022-08-02
期刊：
Cells
影响因子：
6
作者：
通讯作者：

Trophectoderm formation: regulation of morphogenesis and gene expressions by RHO, ROCK, cell polarity, and HIPPO signaling.

DOI：
10.1530/rep-21-0478
发表时间：
2022-10-01
期刊：
REPRODUCTION
影响因子：
3.8
作者：
Alarcon, Vernadeth B.;Marikawa, Yusuke
通讯作者：
Marikawa, Yusuke

Exosome-Enriched Plasma Analysis as a Tool for the Early Detection of Hypertensive Gestations.

DOI：
10.3389/fphys.2021.767112
发表时间：
2021
期刊：
Frontiers in physiology
影响因子：
4
作者：
Weingrill RB;Paladino SL;Souza MLR;Pereira EM;Marques ALX;Silva ECO;da Silva Fonseca EJ;Ursulino JS;Aquino TM;Bevilacqua E;Urschitz J;Silva JC;Borbely AU
通讯作者：
Borbely AU

Efficient deletion of LoxP-flanked selectable marker genes from the genome of transgenic pigs by an engineered Cre recombinase.

DOI：
10.1007/s11248-020-00200-3
发表时间：
2020-06
期刊：
Transgenic research
影响因子：
3
作者：
Huang X;Zou X;Xu Z;Tang F;Shi J;Zheng E;Liu D;Moisyadi S;Urschitz J;Wu Z;Li Z
通讯作者：
Li Z

Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.

DOI：
10.1186/s40104-022-00754-8
发表时间：
2022-09-17
期刊：
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
影响因子：
7
作者：
Ribas-Maynou, Jordi;Delgado-Bermudez, Ariadna;Mateo-Otero, Yentel;Vinolas, Estel;Hidalgo, Carlos O.;Ward, W. Steven;Yeste, Marc
通讯作者：
Yeste, Marc