Full polarization characterization by OCT

通过 OCT 进行全偏振表征

• 批准号: 6735633
• 负责人: Lihong Wang
• 金额: $ 32.37万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6735633
• 关键词: animal tissue; bioimaging /biomedical imaging; burns; chemical carcinogenesis; diagnosis design /evaluation; fiber optics; histology; image enhancement; laboratory mouse; light microscopy; miniature swine; noninvasive diagnosis; optical polarization; optical tomography; skin neoplasms; technology /technique development; wound healing

DESCRIPTION (provided by applicant): The long-term objective of the proposed research is to develop a novel, non-invasive tool for microscopic imaging of superficial lesions, including cancers and burns. The short-term goal is to apply the proposed technology in small-animal imaging. The proposed technique, Mueller-matrix optical coherence tomograph, (OCT), can image in real time the complete polarization properties of intact biological tissues for the first time at the microscopic scale (about 10 microns) in vivo. Optical polarization properties are sensitive indicators of physiological states such as the collagen content and abnormalities of biological tissues such as necrosis, and they can provide novel contrast mechanisms for imaging. Initially, this technology will likely have an impact on small-animal experimental studies because it can reduce the number of animals needed and the time required to conduct a study and can also improve the temporal correlation of a study. In addition to the immediate applications, this technology has the potential to detect various superficial human diseases--such as oral, skin, cervical, colon, and bladder cancers as well as skin burns--that can be accessed either directly or endoscopically as already demonstrated by conventional OCT. Mueller matrices can completely characterize the polarization properties of any material. The applicants' group pioneered Mueller-matrix OCT and demonstrated that this new imaging modality reveals tissue structures that are not observable with conventional OCT. Striking polarization contrast has already been shown in burns by Mueller-matrix OCT. The specific aims of the proposed research are as follows, in which the animal experiments will have dual foci: the imaging of skin cancers (animal model 1) and the imaging of burns (animal model 2). Aim 1. Develop a free-space Mueller-matrix OCT system to image Mueller matrices of biological tissues with both depth and lateral resolutions in real time. Aim 2. Characterize the capability of the proposed system and understand the origin of OCT polarization contrast by imaging tissue samples ex vivo. Compare the Mueller-matrix images with the corresponding histological results from both conventional and polarization light microscopes and identify the relationships between the Mueller-matrix images and the histological structures. Aim 3. Further develop the free-space Mueller-matrix OCT system using fiber optics and construct a hand-held probe for in vivo applications. Compare the experimental results from the fiber-optic system with those from the original free-space system. Aim 4. Characterize the capability of the proposed hand-held probe by imaging skin cancers in vivo in a mouse model (animal model 1). Detect the location, size, and Mueller-matrix contrast of skin cancers in comparison with the histological results from both conventional and polarization light microscopes. Analyze and characterize the temporal progression of skin cancer in the animal model. Aim 5. Characterize the capability of the proposed hand-held probe by imaging skin burns in vivo in a mini-pig model (animal model 2). Detect the lateral extent, depth, and Mueller-matrix contrast of skin burns in comparison with the histological results from both conventional and polarization light microscopes. Analyze and characterize the temporal healing process of burns in the animal model.

描述（由申请人提供）：拟议的研究的长期目标是开发一种新型的无创工具，用于对包括癌症和烧伤在内的浅表病变的微观成像。短期目标是将所提出的技术应用于小动物成像。所提出的技术，即Mueller-Matrix光学相干断层扫描（OCT），可以实时成像完整生物组织的完整极化特性，首次在体内微观尺度（约10微米）。光学极化特性是生理状态的敏感指标，例如胶原蛋白含量和生物组织的异常，例如坏死，它们可以为成像提供新颖的对比机制。最初，该技术可能会对小动物实验研究产生影响，因为它可以减少所需的动物数量以及进行研究所需的时间，还可以改善研究的时间相关性。除了直接应用外，该技术还具有检测各种浅表人类疾病（例如口服，皮肤，宫颈，结肠和膀胱癌）以及皮肤燃烧的可能性 - 可以直接或内镜检查，如常规OCT所示。 Mueller矩阵可以完全表征任何材料的极化特性。申请人的群体开创性的穆勒 - 马trix OCT，并证明了这种新成像方式揭示了与常规OCT无法观察到的组织结构。 Mueller-Matrix Oct在Burns中已经显示出惊人的极化对比。拟议的研究的具体目的如下，在该目的中，动物实验将具有双重焦点：皮肤癌的成像（动物模型1）和烧伤的成像（动物模型2）。 AIM 1。开发一个自由空间的Mueller-Matrix OCT系统，以实时对具有深度和横向分辨率的生物组织的Mueller矩阵进行成像。 AIM 2。表征所提出的系统的能力，并通过成像组织样品在体内成像对比度的起源。将Mueller-Matrix图像与传统和极化光显微镜的相应组织学结果进行比较，并确定Mueller-Matrix图像与组织学结构之间的关系。目标3。进一步使用光纤开发自由空间Mueller-Matrix OCT系统，并为体内应用构建手持探针。将光纤系统的实验结果与原始自由空间系统的实验结果进行比较。 AIM 4。通过在小鼠模型中对体内的皮肤癌进行成像（动物模型1）来表征所提出的手持探针的能力。与传统光显微镜和极化光显微镜的组织学结果相比，检测皮肤癌的位置，大小和穆勒矩阵对比。分析和表征动物模型中皮肤癌的时间进展。 AIM 5。通过在迷你钉模型中成像皮肤在体内成像燃烧，以表征所提出的手持探针的能力（动物模型2）。与常规光显微镜和极化光显微镜的组织学结果相比，检测皮肤燃烧的横向程度，深度和穆勒矩阵对比度。分析和表征动物模型中烧伤的时间愈合过程。