IN VIVO IMAGING OF SINGLE CIRCULATING CELLS

单循环细胞的体内成像

基本信息

批准号：
8739639
负责人：
Lihong Wang
金额：
$ 56.51万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-23 至 2018-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8739639
关键词：
Acoustics Animals Biological Blood Vessels Blood Volume Blood flow Cancer Patient Cells Clinical Clinical Research Confocal Microscopy Detection Diffusion Disease Early Diagnosis Ensure Erythrocytes Extravasation Fluorescence Microscopy Goals Health Hazards Hemoglobin Human Image Imaging technology Label Laboratories Length Light Malignant Neoplasms Melanins Metabolic Metabolism Microscopic Microscopy Molecular Monitor Neoplasm Circulating Cells Neoplasm Metastasis Operative Surgical Procedures Optical Coherence Tomography Optics Organ Organelles Oxygen Oxyhemoglobin Pharmaceutical Preparations Radiation Research Resolution Screening for cancer Skin Staging Structure System Systems Biology Technology Therapeutic Therapeutic Intervention Time Tissues Translating Translations Tumor Oxygenation Ultrasonic wave Ultrasonics Ultrasonography absorption base cellular imaging clinical practice deoxyhemoglobin design fluorescence imaging improved in vivo in vivo imaging innovation melanoma molecular imaging neoplastic cell optical imaging outcome forecast photoacoustic imaging pre-clinical public health relevance response sound spatiotemporal tomography tumor two-photon

项目摘要

DESCRIPTION (provided by applicant): The goal of the proposed research is to translate single-cell label-free photoacoustic microscopy (PAM) into clinical practice. In vivo PAM has been invented for early-cancer detection and functional, metabolic, or molecular imaging by physically integrating optical and ultrasonic waves. Unlike ionizing x-ray radiation, light poses n health hazard and reveals molecular contrasts. Unfortunately, light does not penetrate biological tissue in straight paths as x-rays do. Consequently, high-resolution optical imaging-such as confocal microscopy, two-photon microscopy, and optical coherence tomography-has been restricted to tissue depths within the optical diffusion limit (~1 mm in the skin). PAM breaks through this limitation by taking advantage of the fact that ultrasonic scattering per unit path-length in tissue is ~1000 times less than optical scattering. It is exquisitely sensitive to optica absorption contrasts, enabling it to image far more molecules than fluorescence microscopy. PAM may hold the key to the earliest detection of cancer by in vivo label-free quantification of hyper-metabolism, the quintessential hallmark of cancer. The proposed immediate clinical translation of this technology will enable in vivo imaging and detection of single circulating cell, especially circulating tumor cells for cancer screening, detection, prognosis, and monitoring. We propose the following specific aims to clinically translate PAM. Aim 1. High-resolution label-free PAM for in vivo imaging of circulating single red blood and tumor cells. Aim 2. High-throughput label-free PAM for in vivo imaging of single circulating tumor cells (CTCs).

描述（由申请人提供）：拟议的研究的目的是将无单细胞标签的光声显微镜（PAM）转化为临床实践。通过物理整合光学和超声波，用于早期癌症检测以及功能，代谢或分子成像的发明体内PAM。与电离X射线辐射不同，光构成N危险，并揭示了分子对比。不幸的是，光不会像X射线那样在直线路径中穿透生物组织。因此，高分辨率的光学成像，例如共聚焦显微镜，两光子显微镜和光学相干断层扫描术，仅限于光学扩散极限内的组织深度（皮肤中〜1 mm）。 PAM通过利用这样的事实，即每单位路径长度在组织中的超声散射比光学散射小约1000倍。它对Optica的吸收对比非常敏感，使其能够比荧光显微镜更大的分子成像。 PAM可以通过体内无标签的超级代谢（癌症的典型标志）对癌症进行最早检测的关键。提出的该技术的即时临床翻译将使单个循环细胞的体内成像和检测能够在体内进行检测，尤其是循环肿瘤细胞进行癌症筛查，检测，预后和监测。我们提出以下特定目的，以临床翻译PAM。 AIM1。高分辨率的无标签PAM，用于循环单个红血和肿瘤细胞的体内成像。 AIM 2。高通量标签无PAM用于单个循环肿瘤细胞（CTC）的体内成像。