Fast high-resolution deep photoacoustic tomography of action potentials in brains

大脑动作电位的快速高分辨率深度光声断层扫描

基本信息

批准号：
9423398
负责人：
Lihong Wang
金额：
$ 26.46万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-02-04 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9423398
关键词：
Fast resolution deep photoacoustic tomography

项目摘要

DESCRIPTION (provided by applicant): Revealing how our brain works is a great challenge but yet worth our every effort: it will not only illuminate the profound mysteries in science but also provide the key to understanding and treating neurological diseases such as Alzheimer's and Parkinson's. The objective of the proposed three-year research is to develop a high- speed, high-spatial-resolution, deep-penetration photoacoustic computed tomography (PACT) system for real- time imaging of action potentials in mouse brains. The proposed hardware imaging system will be unprecedented in the field of PACT in terms of frame rate and spatial resolving power, and the proposed use of voltage-sensitive absorption dyes in PACT is also novel. In comparison to existing high-resolution optical neuroimaging modalities such as two-photon microscopy, the proposed system will provide deeper penetration for whole mouse brain imaging. The timing for such an exciting project is perfect due to the following recent events. Our unpublished ongoing works have shown for the first time that (1) PACT has reached x-ray CT like image quality (see images in the Aim 2 section) and (2) some of the voltage-sensitive fluorescent dyes provide excellent photoacoustic contrast (see data in the Aim 1 section). The ultrasound imaging industry has just started to sell standalone multi-channel data acquisition systems without bundling to a conventional linear-array ultrasonographic system that we do not need, and the users are given full access to the raw RF data. Massively parallel (512 channels) data acquisition enables real-time PACT. In addition to the RFA, the March publication in the Journal of Biomedical Optics by NIH Program Director, Dr. Jonathan D. Pollock, entitled "Deep imaging technology needed for NIH BRAIN initiative,"[1] urged our team to submit this grant application. The specific aims include 1. Screen voltage-sensitive probes for photoacoustic imaging in cell culture preparations and optimize detection parameters. 2. Develop a fast, high-resolution, deep-penetration PACT system. 3. Use PACT to image action potentials in mouse brains in vivo.

描述（由适用提供）：揭示我们的大脑的工作方式是一个巨大的挑战，但值得我们努力：它不仅会阐明科学中深刻的奥秘，而且还为理解和治疗神经系统疾病（例如阿尔茨海默氏症和帕金森氏症）提供了关键。拟议的三年研究的目的是开发高速，高空间分辨率，深度渗透光声计算机断层扫描（PACT），在PACT领域中，建议在框架和空间解析功率方面，将在PACT领域毫无疑问，并提出了在Pact中的拟议中使用Pact in pact in pact。与现有的高分辨率光学神经影像模式（如两光子显微镜）相比，所提出的系统将为整个小鼠脑成像提供更深的渗透。由于最近的事件，这种令人兴奋的项目的时机非常完美。我们未发表的正在进行的作品首次表明（1）条约已达到X射线CT，例如图像质量（请参阅AIM 2部分中的图像）和（2）某些电压敏感的荧光染料提供了出色的光声对比（请参阅AIM 1部分中的数据）。超声成像行业刚刚开始销售独立的多渠道数据采集系统，而无需捆绑到我们不需要的传统线性阵列超声检查系统，并且用户可以完全访问原始RF数据。大规模平行（512个通道）数据采集可以实时协议。除RFA外，NIH计划主管Jonathan D. Pollock博士在《生物医学光学杂志》中的三月出版物，题为“ NIH Brain Suniative所需的深层成像技术”，[1]敦促我们的团队提交此赠款申请。具体目的包括1。屏幕电压敏感问题，用于细胞培养准备中的光声成像并优化检测参数。 2。发展一个快速，高分辨率，深度渗透协议系统。 3。使用Pact在体内图像小鼠大脑中的动作电位。