DIETARY CONTENT & GASTROINTESTINAL ABSORPTION OF D CHIRO INOSITOL & PINITOL

膳食含量

• 批准号: 6665877
• 负责人: RICHARD E. OSTLUND
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665877
• 关键词: biological products; biomedical resource; human tissue; immunology; inflammation; proteins; spectrometry

D-chiro-Inositol-containing compounds were abundant in common laboratory animal feed. By gas chromatography-mass spectrometry (GC/MS) of 6 N HCI hydrolysates, Purina Roden Chow 5001 contained 0.23% of D-chiro-inositol by weight. The principal sources were alfalfa and soybean meal whereas other chow ingredients had less than 2% as much per gram as these legumes. Only traces of L-chiro-inositol were found. D-chiro-Inositol was present in a water-soluble non-ionic form of which most was shown to be pinitol, 1D-3-O-methyl-chiro-inositol. More than 98% of the D-chiro-Inositol fed to rats as Purina Rodent Chow 5001 as well as purified pinitol and deuterated D-chiro-Inositol tracer was absorbed from the gastrointestinal tract. Rats chronically ingesting the chow diet consumed 921 (mol of total D-chiro-inositol/kg/day and excreted less than 5% of that amount in the stool and urine suggesting that the bulk was metabolized. However, even though urinary excretion of total D-chiro-i nositol was very small compared to intake, it declined further by over 98% in response to a chiro-inositol-deficient diet. Thus, urinary levels may reflect dietary intake. Recent reports have shown that D-chiro-inositol is found in certain putative insulin mediator fractions and that increased urinary excretion occurs in uncontroleld diabetes mellitus. Further work is needed to clarify the dietary and potential nutritive role of D-chiro-inositol.

含有丰富的 D-手性肌醇化合物的共同点 实验动物饲料。 通过气相色谱-质谱法 (GC/MS) 6 N HCl 水解产物，含有 Purina Roden Chow 5001 0.23重量％的D-手性肌醇。 主要来源是 苜蓿和豆粕，而其他饲料原料的含量少于 每克含量比这些豆类低 2%。 仅痕量 L-手性肌醇 被发现。 D-手性肌醇以水溶性非离子形式存在 其中大多数形式被证明是松醇， 1D-3-O-甲基-手性肌醇。 98%以上为D-手性肌醇 以 Purina Rodent Chow 5001 以及纯化的松醇和纯化的松醇喂养大鼠 氘代 D-手性肌醇示踪剂从 胃肠道。 长期摄入食物的老鼠 消耗 921（mol 总 D-手性肌醇/kg/天，排出量较少 粪便和尿液中的含量超过该量的 5%，表明大部分 被代谢了。 然而，尽管尿排泄总量 D-chiro-i 鼻醇与摄入量相比非常小，因此下降 进一步超过 98% 对缺乏手性肌醇的饮食有反应。 因此，尿液水平可能反映饮食摄入量。 最近的报道有 表明在某些假定的胰岛素中发现了 D-手性肌醇 介质分数和尿排泄增加发生在 未控制的糖尿病。 需要进一步的工作来澄清 D-手性肌醇的膳食和潜在营养作用。