THE EFFECTS OF PHYTOSTEROLS PRESENT IN NATURAL FOOD MATRICES ON CHOLESTEROL

天然食品基质中存在的植物甾醇对胆固醇的影响

• 批准号: 8361451
• 负责人: RICHARD E. OSTLUND
• 金额: $ 1.31万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361451
• 关键词: Cholesterol; Cholesterol Homeostasis; Diet; Excretory function; Food; Funding; Grant; Intestines; Investigation; LDL Cholesterol Lipoproteins; Mass Fragmentography; Mass Spectrum Analysis; Metabolic; Methods; National Center for Research Resources; Nutrient; Oral Administration; Phytosterols; Plasma; Principal Investigator; Randomized; Reducing diet; Research; Research Infrastructure; Resources; Source; Testing; Tracer; United States National Institutes of Health; biomedical resource; cholesterol absorption; cost; feeding

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Background/Objectives:Extrinsic phytosterols supplemented to the diet reduce intestinal cholesterol absorption and plasma low-density lipoprotein (LDL)-cholesterol. However, little is known about their effects on cholesterol metabolism when given in native, unpurified form and in amounts achievable in the diet. The objective of this investigation was to test the hypothesis that intrinsic phytosterols present in unmodified foods alter whole-body cholesterol metabolism.Subjects/Methods:In all, 20 out of 24 subjects completed a randomized, crossover feeding trial wherein all meals were provided by a metabolic kitchen. Each subject consumed two diets for 4 weeks each. The diets differed in phytosterol content (phytosterol-poor diet, 126 mg phytosterols/2000 kcal; phytosterol-abundant diet, 449 mg phytosterols/2000 kcal), but were otherwise matched for nutrient content. Cholesterol absorption and excretion were determined by gas chromatography/mass spectrometry after oral administration of stable isotopic tracers.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 背景/目标：饮食中补充的外源植物甾醇可减少肠道胆固醇吸收和血浆低密度脂蛋白 (LDL) 胆固醇。然而，人们对它们以天然、未纯化的形式以及在饮食中可达到的量给予时对胆固醇代谢的影响知之甚少。本次调查的目的是检验未经改良食品中存在的内在植物甾醇会改变全身胆固醇代谢的假设。 受试者/方法：总共 24 名受试者中有 20 名完成了一项随机交叉喂养试验，其中所有膳食均由新陈代谢的厨房。每个受试者都食用两种饮食，每次 4 周。这些饮食的植物甾醇含量不同（植物甾醇含量低的饮食，126毫克植物甾醇/2000大卡；植物甾醇丰富的饮食，449毫克植物甾醇/2000大卡），但在其他方面的营养成分是匹配的。口服稳定同位素示踪剂后，通过气相色谱/质谱法测定胆固醇的吸收和排泄。