Islet Procurement and Analysis Core

胰岛获取和分析核心

• 批准号: 9049481
• 负责人: Marcela Brissova
• 金额: $ 10.26万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-04-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9049481
• 关键词: Area; Biological Assay; Biology; Development; Diabetes Mellitus; Equipment; Hormones; Human; Image Analysis; Immunology; Individual; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Knowledge; Measurement; Morphology; Mus; Postdoctoral Fellow; Price; Procedures; Process; Quality Control; RNA; Reporting; Research; Research Personnel; Scanning; Services; Signal Transduction; Slide; System; Techniques; Technology; Training; base; experience; graduate student; islet; morphometry; pancreatic islet function; tool; transcription factor

The Islet Procurement and Analysis Core (IPA Core) was expanded and became a new core of the Vanderbilt DRTC in June 2007. Soon after that the IPA Core was integrated into the Vanderbilt Core Ordering & Reporting Enterprise System. Islet biology, development, and function are major research areas of the Vanderbilt DRTC with investigators studying a variety of islet-related processes ranging from intracellular signaling, the immunology of type 1 diabetes, islet-enriched transcription factors, to islet transplantation. Necessary components of many of their experimental paradigms are high quality, well-characterized pancreatic islets, and reliable assays of islet function and mass. The main objective of the IPA Core is to provide DRTC-affiliated investigators with high quality mouse pancreatic islets. In addition, the core assists investigators in studies of islet function that involve services such as islet culture, islet perifusion, static islet incubation and RNA isolation. An important component of the IPA Core services is training. The IPA Core staff will continue to provide training to graduate students and postdoctoral fellows thus allowing them to gain knowledge and understanding of procedures used in their research. Due to high demand among DRTC-affiliated investigators, the IPA Core added to its equipment Aperio whole slide scanning system and will expand its service portfolio for the assessment of islet mass/morphology in this competitive renewal cycle. This will not only broaden the current DRTC user base, but will also allow us to reach out to non-VUMC users and offer slide scanning and image analysis services. Utilizing Aperio whole slide scanning technology and image analysis tools, the IPA Core will develop standards for pancreatic islet morphometry and measurement of islet mass. By having a centralized facility, the IPA Core provides services to a larger base of investigators at a lower price than would otherwise be possible. Other advantages are quality control, the ability to develop new techniques and applications, the increased potential for collaborative interactions, and immediate access to individuals with a broad experience and knowledge in handling isolated islets.

Islet 采购和分析核心（IPA 核心）于 2007 年 6 月进行了扩展，成为范德比尔特 DRTC 的新核心。不久之后，IPA 核心被集成到范德比尔特核心订购和报告企业系统中。胰岛生物学、发育和功能是范德比尔特 DRTC 的主要研究领域，研究人员研究各种胰岛相关过程，包括细胞内信号传导、1 型糖尿病的免疫学、富含胰岛的转录因子和胰岛移植。他们的许多实验范例的必要组成部分是高质量、特征良好的胰岛，以及可靠的胰岛功能和质量测定。 IPA Core 的主要目标是为 DRTC 附属研究人员提供高质量的小鼠胰岛。此外，该核心还可以协助研究人员进行胰岛功能研究，包括胰岛培养、胰岛灌注、静态胰岛孵育和 RNA 分离等服务。 IPA 核心服务的一个重要组成部分是培训。 IPA 核心工作人员将继续为研究生和博士后研究员提供培训，使他们能够获得对其研究中使用的程序的知识和理解。由于 DRTC 附属研究人员的高需求，IPA Core 在其设备中添加了 Aperio 全玻片扫描系统，并将在这个竞争性更新周期中扩展其用于评估胰岛质量/形态的服务组合。这不仅将扩大当前的 DRTC 用户群，而且还将使我们能够接触到非 VUMC 用户并提供幻灯片扫描和图像分析服务。利用 Aperio 全玻片扫描技术和图像分析工具，IPA Core 将制定胰岛形态测量和胰岛质量测量的标准。通过拥有集中式设施，IPA 核心可以以比其他方式更低的价格为更多的调查人员提供服务。其他优势包括质量控制、开发新技术和应用的能力、协作互动潜力的增加，以及立即接触到在处理孤立小岛方面拥有丰富经验和知识的个人。