Islet and Pancreas Analysis Core

胰岛和胰腺分析核心

• 批准号: 10408483
• 负责人: Marcela Brissova
• 金额: $ 16.27万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10408483
• 关键词: Abbreviations; Academic Medical Centers; Achyrocline; Aging; Animals; Architecture; Area; Biological Assay; Biology; Cell Differentiation process; Cells; Cellular Morphology; Complement; Complex; Constitution; Detection; Development; Diabetes Mellitus; Disease; Functional disorder; Funding; Genetic; Histologic; Homeostasis; Hormone secretion; Hormones; Human; Image; Image Analysis; Image Enhancement; Immunology; In Vitro; Individual; Infrastructure; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Location; Maintenance; Measurement; Metabolic; Metabolism; Molecular; Molecular Abnormality; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Non-Insulin-Dependent Diabetes Mellitus; Pancreas; Phenotype; Physiological; Physiology; Procedures; Process; Publishing; Radioimmunoassay; Research; Research Personnel; Resolution; Resources; Rodent; Services; Signal Transduction; Standardization; Techniques; Technology; Tissues; Training; Work; analytical tool; base; bioimaging; blood glucose regulation; cell type; cost; endocrine pancreas development; flexibility; human tissue; imaging modality; in vivo; indexing; islet; metabolic phenotype; multiplexed imaging; pancreas development; physiologic stressor; programs; protein expression; response; success; transcription factor; whole slide imaging

Islet and Pancreas Analysis Core: Project Summary/Abstract The Islet and Pancreas Analysis (IPA) Core was established in 2007 as a result of growing demand for murine islets from Vanderbilt Diabetes Research Center (VDRC) investigators. Over the last funding cycle, the IPA Core provided services to 47 different investigators (40 Vanderbilt and 7 external nonprofit) by assisting with 4,120 procedures. Research programs within the VDRC encompass the fields of pancreatic islet biology, development, and function in normal homeostasis and disease, including a broad spectrum of islet-related processes raging from intracellular signaling, physiological response and adaptation, the immunology of type 1 diabetes, the function of islet-enriched transcription factors, and the maintenance of normal hormone-secreting status and cell fate. Essential to rigorous research in these areas is the ability to robustly isolate murine islets and to assay the complex physiological function of both murine and human islets. Vanderbilt has been a national leader in developing islet functional assays, as evidenced by the selection of the NIDDK-supported Integrated Islet Distribution Program (IIDP) to house the Human Islet Phenotyping Program (HIPP) at Vanderbilt under direction of Marcela Brissova, IPA Core Director. The HIPP's location at Vanderbilt has greatly benefitted VDRC Investigators, allowing newly developed procedures to be seamlessly implemented through the IPA Core. In addition to offering functional assays, the IPA Core also provides VDRC investigators with high-resolution, whole-slide image scanners that enable simultaneous analysis of both tissue architecture and single cell morphology. Numerous VDRC investigators utilize the imaging infrastructure and associated analytical tools, and in this competitive renewal, the IPA Core proposes the addition of multiplex imaging to perform high impact, tissue-based studies. While access to this technology is cost-prohibitive and technically challenging for individual labs, offering it as a core service will significantly enhance the impact of histological analysis of mouse and human tissue. The IPA Core is also working closely with the Animal Metabolic Physiology Core (AMPC) to standardize islet transplantation techniques, a valuable experimental resource that will complement existing ex vivo assays and allow more investigators to perform in vivo studies. Through its centralized resources and expertise, the IPA Core provides VDRC investigators with reliable and standardized procedures that facilitate discoveries related to islet biology, cell signaling, and metabolism. The continued excellence of the IPA Core is crucial to sustain rigorous and impactful work in the areas of pancreas and islet development and physiology by VDRC investigators.

胰岛和胰腺分析核心：项目摘要/摘要 由于对小鼠的需求不断增长，胰岛和胰腺分析 (IPA) 核心于 2007 年成立 来自范德比尔特糖尿病研究中心 (VDRC) 研究人员的胰岛。在上一个融资周期中，IPA Core 通过协助 47 名不同的调查员（40 名范德比尔特和 7 名外部非营利组织）提供服务 4,120 道程序。 VDRC 的研究项目涵盖胰岛生物学领域， 正常稳态和疾病中的发育和功能，包括广泛的胰岛相关 细胞内信号传导、生理反应和适应、1 型免疫学的激烈过程 糖尿病、富含胰岛的转录因子的功能以及正常激素分泌的维持 状态和细胞命运。这些领域的严格研究的关键是能够可靠地分离小鼠胰岛 并测定小鼠和人类胰岛的复杂生理功能。范德比尔特一直是 NIDDK 支持的选择证明了在开发胰岛功能测定方面的国家领导者 综合胰岛分布计划（IIDP）将人类胰岛表型计划（HIPP）安置在 范德比尔特在 IPA 核心总监 Marcela Brissova 的指导下。 HIPP 位于范德比尔特 VDRC 调查人员受益匪浅，可以无缝实施新开发的程序 通过 IPA 核心。除了提供功能测定之外，IPA 核心还为 VDRC 研究人员提供 配备高分辨率全玻片图像扫描仪，可同时分析两种组织结构 和单细胞形态。许多 VDRC 调查人员利用成像基础设施和相关的 分析工具，在这次竞争更新中，IPA 核心建议添加多重成像 进行高影响力的、基于组织的研究。虽然获得这项技术的成本高昂且技术上 对于单个实验室来说是一项挑战，将其作为核心服务提供将显着增强组织学的影响 小鼠和人体组织的分析。 IPA Core 还与动物代谢中心密切合作 生理学核心 (AMPC) 用于标准化胰岛移植技术，这是一种宝贵的实验资源 将补充现有的离体测定，并允许更多的研究人员进行体内研究。通过其 集中资源和专业知识，IPA 核心为 VDRC 调查人员提供可靠且标准化的信息 促进与胰岛生物学、细胞信号传导和新陈代谢相关的发现的程序。继续的 IPA 核心的卓越性对于维持胰腺和胰岛领域严格且有影响力的工作至关重要 VDRC 研究人员对发育和生理学进行了研究。