Advanced Mass Spectrometry Facility

先进的质谱设备

• 批准号: 10712585
• 负责人: David Anderson
• 金额: $ 141.55万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10712585
• 关键词: Adopted; Annual Reports; Area; Back; Biological; Biopolymers; Calibration; Communities; Complex; Computer software; Core Facility; Cost Effectiveness Analysis; Data; Data Analyses; Extramural Activities; Federal Government; Gel; Label; Link; Mass Spectrum Analysis; Measurement; Measures; Methodology; Methods; Modification; Molecular; Molecular Structure; Molecular Weight; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Drug Abuse; Natural Products; Outsourcing; Peptide Fragments; Peptides; Philosophy; Play; Problem Solving; Proteins; Reporting; Research; Research Project Grants; Resolution; Resources; Rice; Role; Running; Sampling; Science; Software Tools; Structure; Synthesis Chemistry; System; Techniques; Time; Update; analysis pipeline; base; crosslink; dalton; experience; experimental study; instrument; macromolecule; mass spectrometer; novel strategies; protein complex; small molecule; tool

Historically the facility has supported on the order of 40 PIs each year involved with around 70 annual report level projects with around two-thirds of these projects coming from the NIDDK. Although useful metrics for some things, the PI-count and project-count are oversimplifications. It is important to note that the amount of support provided to each project varies widely. For instance, support of small molecule chemists at NIDDK and a number of projects involving biopolymer analysis at NIDDK were very involved while most of the support provided to non-NIDDK labs involved one or two low staff time experiments. Support of Kenner Rice (NIDA) is a special case as he only recently moved to NIDA from NIDDK and has had a long-standing working relationship with the facility in all its historical forms. In addition to working in direct support of PI projects staff and resources are used to update methodology, adopting new and proven approaches of clear importance to the NIDDK PI community and in a limited number of cases developing new approaches when these are of immediate importance to on-going PI projects and otherwise consistent with the facility's technical philosophies. Continued the long internal project of developing a reliable XL-MS analysis pipeline with a successful solution of the quantitation problem with an original effort of refactoring of software for the inclusion of observations reported by the developers of MaxQuant regarding recognition of DSSO linked peptides' fragmentation suspended because of there being no clear advantage relative to our internal+mascot approach and faiure of the MaxQuant option to allow modification (perhaps this will change in newer versions). Adopted and refined a new method for extracting proteins from gels based on PEPPI (but modified to make it more predictable). Applied this to several projects (for example with Andy Golden and Harris Bernstein).

从历史上看，该设施每年都支持40个PI的订单，其中约有70个年度报告级项目，其中约有三分之二来自NIDDK。 尽管在某些方面有用的指标，但PI计数和项目计数是过分的。重要的是要注意，为每个项目提供的支持量差异很大。例如，NIDDK的小分子化学家的支持以及涉及NIDDK生物聚合物分析的许多项目非常涉及，而提供给非NIDDK实验室的大多数支持涉及一个或两个低的员工时间实验。肯纳·赖斯（Kenner Rice）（NIDA）的支持是一个特殊情况，因为他最近才从NIDDK搬到NIDA，并且与该设施的所有历史形式都有长期的工作关系。 除了直接支持PI项目外，还使用资源来更新方法论，采用新的且经过验证的方法对NIDDK PI社区的重要性，并且在有限的情况下，在有限的情况下开发了新方法，这至关重要。 - 正在进行的PI项目，否则与该设施的技术理念一致。 继续进行了漫长的内部项目，该项目通过成功地解决了定量问题的解决方案，以重构软件的原始努力，以包含Maxquant开发人员在识别DSSO链接肽段悬挂DSSO链接的碎片暂停的观察方面的原始努力。由于我们的内部+吉祥物方法和最大选项的faiure没有明显的优势来允许修改（也许这会在较新版本中改变）。采用并完善了一种基于PEPPI从凝胶中提取蛋白质的新方法（但修改以使其更可预测）。将其应用于几个项目（例如与安迪·戈尔登（Andy Golden）和哈里斯·伯恩斯坦（Harris Bernstein）一起使用）。