Advanced Mass Spectrometry Facility

先进的质谱设备

• 批准号: 8554135
• 负责人: David Anderson
• 金额: $ 261.47万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8554135
• 关键词: 1,2-diacylglycerol; Agonist; Alcohol abuse; Alzheimer' s Disease; Amyloid beta-Protein; Animal Model; Anthrax disease; Antigens; Archives; Area; Bacillus anthracis; Bacteriophage T4; Binding; Biogenesis; Biogenic Amine Receptors; Biological; Biological Factors; Biopolymers; Caenorhabditis elegans; Calibration; Capsid Proteins; Carbohydrates; Cell fusion; Cells; Centrosome; Complex; Core Facility; Corticotropin-Releasing Hormone Receptors; DAG/PE-Binding Domain; DNA; DNA Damage; Data; Data Analyses; Development; Diabetes Mellitus; Diglycerides; Drug Delivery Systems; Drug resistance; Embryo; Epidermal Growth Factor Receptor; Escherichia coli; Federal Government; Focal Segmental Glomerulosclerosis; GTP-Binding Proteins; Gene Expression; Genetic; Glioma; Goals; Golgi Apparatus; Growth and Development function; HIV; HIV Antibodies; HIV Receptors; HIV-1; Habits; Hallucinogens; Hemagglutinin; Hepatitis C; Hepatitis C virus; Human; Immune response; Immunochemistry; Individual; Integral Membrane Protein; Ion Channel; Iron; Label; Learning; Ligands; Malaria; Mammals; Mass Spectrum Analysis; Measurement; Measures; Meiosis; Meiotic Recombination; Membrane; Membrane Fusion; Membrane Proteins; Metabolism; Metric; Molecular; Molecular Biology; Molecular Genetics; Molecular Structure; Molecular Weight; National Institute of Dental and Craniofacial Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Drug Abuse; National Institute of Neurological Disorders and Stroke; National Institute on Alcohol Abuse and Alcoholism; Nuclear; Nucleic Acids; Obesity; Oocytes; Opioid Receptor; Parasites; Paste substance; Pathogenesis; Pathway interactions; Patient Self-Report; Pattern recognition receptor; Peptide Fragments; Peptide Nucleic Acids; Peripheral; Pharmaceutical Chemistry; Pharmaceutical Preparations; Phosphorylation; Phosphotransferases; Phylogeny; Play; Post-Translational Protein Processing; Principal Investigator; Problem Solving; Proteins; Publications; Purinergic P1 Receptors; RGS Proteins; RNA Folding; Reaction; Receptor Signaling; Recording of previous events; Regulation; Reporting; Research; Research Project Grants; Resolution; Resources; Rice; Role; Running; SPO11 gene; Sampling; Science; Signal Pathway; Signal Transduction; Small RNA; Software Tools; Solutions; Sorting - Cell Movement; Structure; Structure-Activity Relationship; Synthesis Chemistry; System; Techniques; Thyrotropin; Thyrotropin Receptor; Thyrotropin-Releasing Hormone; Thyrotropin-Releasing Hormone Receptors; Time; Toll-like receptors; Transferrin; Transforming Growth Factor beta; Transposase; Tuberculosis; Tumor Suppressor Genes; Ubiquitin; United States National Institutes of Health; Vesicle; Virulence; Virulence Factors; Work; Yersinia pestis; alpha synuclein; autoimmune lymphoproliferative syndrome; chemical genetics; computerized data processing; crosslink; dalton; design; drug development; experience; glutathionylspermidine; histone acetyltransferase; indexing; inhibitor/antagonist; instrument; interest; macromolecule; mass spectrometer; monoamine; mutant; novel; protein complex; protein transport; receptor; research facility; research study; response; small molecule; solid state nuclear magnetic resonance; therapeutic vaccine; tool; uptake; yeast prion

FORTY Principal Investigators reported that during the 2012 fiscal year 70 federal research projects were supported by the facility. Of this total 46 projects (67%) and 26 PI's(67%) were from NIDDK, 24 projects and 14 PI's were from other parts of NIH (NIAID 12/7, NIDA 7/2 , NCI 2/2, NINDS 1/1, NIDCR 1/1, NIAAA 1/1). Although useful metrics for some things, the PI-count and project-count are oversimplifications. It is important to note that the amount of support provided to each project varies widely. For instance, support of small molecule chemists at NIDDK and a number of projects involving biopolymer analysis at NIDDK (Dean, Kopp, Liang) were very involved while most of the support provided to non-NIDDK labs involved one or two low staff time experiments. Support of Kenner Rice (NIDA) is a special case as he only recently moved to NIDA from NIDDK and has had a long-standing working relationship with the facility in all its historical forms. Please see the individual research reports indexed here to learn more about the important research the facility supports and publication details. NOTE: PI's with asterisks were unavailable during the short time available for self-reports and are included here because files have been archived from these PIs in FY2012 and they have not responded in the negative to the information request and they have history reporting their use - only some of those that did not report explicitly are included. A fully self-reported (and more complete) listing should be available from D. Eric Anderson sometime after September 30th. Index numbers are often misreported due to the habit of cutting and pasting from the previous fiscal year's report information. Daniel Appella DK031123-08 Small Molecule Activators of p53 DK031124-08 Selective Recognition of Folded RNA by Small Oligomers DK031143-08Chemically Modified Peptide Nucleic Acids Clifton Barry (NIAID) AI000783-15 Exploring the metabolism on non-replicating and drug resistant TB AI000693-19 Development of Chemotherapeutics for Tuberculosis Ad Bax DK075023-04 Structural study of the HIV1 gp41 coat protein DK069017-05 Study of hemagglutinin membrane fusion domain DK029047-06 Structure and membrane binding of alpha-synuclein Edward Berger (NIAID) AI000538-24 HIV-Receptor Interactions and Related Anti-HIV Strategies Harris Bernstein DK052037-06 Biogenesis of bacterial autotransporter proteins Carole Bewley DK031135-05 Discovery of Natural Products and Natural Product-Like Inhibitors DK032103-12 Inhibitors and Probes of HIV-1 Cell Fusion Susan Buchanan* DK011011-05 Structural Characterization of Outer Membrane Proteins from Yersinia pestis DK036139-06 Structural Characterization of Bacterial Secretion Channels DK036143-04 Structural Characterization of Iron uptake from Human Transferrin Rafael Camerini-Otero DK052034-07 The role of Spo11 in mammalian meiosis DK052033-07 The different pathways involved in meiotic recombination in mammals Marius Clore DK029038-05 Design of gp41 Directed anti-HIV antibodies DK029023-21 Structure and Dynamics of Macromolecules in Solution by NMR Jurrien Dean* DK015603-06 Molecular Biology of the Oocyte Frederick Dyda* DK036154-05 Structure and function of novel prokaryotic DNA transposase Howard Fine (NCI) BC011101-05 Identifying New Glioma-Associated Tumor Suppressors and Oncogenes Marvin Gershengorn DK011005 To define the structure-function relationships of receptors for thyrotropin-releasing hormone (TRH) (TRH-Rs) and for thyroid-stimulating hormone (TSH-R) with a long-term goal of developing probes/drugs targeted at this class of receptors and to delineate the mechanism(s) of signaling by these receptors DK047044 To discover low molecular weight ligands (agonists and antagonists) for receptors for thyroid-stimulating hormone (TSH-R) and for thyrotropin-releasing hormone (TRH) with a long-term goal of developing probes/drugs targeted at this class of receptors Reinhard K Grisshammer* (NINDS) NS003103-04 Function of Integral membrane proteins Udayan Guha (NCI) (technical assistance) BC011410-02 Protein Phosphorylation Downstream of Mutant EGFR Kinases Deborah M Hinton DK057802-25 Bacteriophage T4 Gene Expression Peggy Hsieh DK052029-06 Cellular Responses to DNA Damage James Hurley DK036123-06 Cargo Sorting and Intralumenal Vesicle Budding by the ESCRT Complexes DK036125-06 Structural Studies of Alix and ESCRT Complexes in HIV-1 Budding DK036126-06 Structural and Functional Studies of Ubiquitin Binding Domains DK036127-06 Mechanisms of Diacylglycerol Signaling Through C1 Domain Proteins DK036128-06 Structural Mechanisms in Retrograde Protein Traffic to the Golgi Ken Jacobson DK031117-24 Development Of Drugs Acting At Adenosine Receptors DK031127-05 Development of Drugs Acting at Ion Channels DK031115-28 Functionalized Congeners Of Bioactive Compounds DK031116-24 Development Of P2Y Receptor Ligands Alan R. Kimmel DK075040-03 Signaling Pathways in Control of Growth and Development Jeffrey Kopp DK043308-17 Focal segmental glomerulosclerosis: genetics and mechanisms Pavol Kovac DK059701-39 Reactions And Immunochemistry Of Carbohydrates Stephen Leppla (NIAID) AI000929-10 Vaccines and Therapeutics for Anthrax AI001030-05 Molecular Genetics and Pathogenesis of Anthrax AI001031-05 Structure and Function of Virulence Factors of Bacillus anthracis AI001032-05 Pathophysiological Actions of Anthrax Virulence Determinants Michael Lenardo (NIAID) AI000565-16 Regulation of Signalling Pathways Involving Nuclear Factor Kapp AI000769-16 Molecular Mechanisms of The Autoimmune Lymphoproliferative Syndrome T. Jake Liang DK054504-16 Cell Cultrure and Animal Models of HCV Infection and HCV Host Interaction Carol Long (NIAID) AI001020-06 Host Immune Responses to Antigens of Malaria Parasites Hans Luecke DK059702-06 Synthesis and Characterization of Selective Histone Acetyltransferase Modulators DK059703-06 Chemical Genetic Elucidation of Histone Acetyltransferase Signaling Networks Aleksandra Nita-Lazar (NIAID)-(help with data processing only) AI001084-04 Protein Modifications Involved in Cell Signaling Amy Newman(NIDA) DA000389-17 Novel Probes for Monoamine Transporters Kevin O'Connell DK024151-10 Centrosome Maturation and Duplication in the C. elegans Embryo Sushil Rane DK055106-06 Transforming Growth Factor Beta Superfamily Signaling in Diabetes and Obesity Kenner Rice(NIDA, once NIDDK) DA000527-05 Medicinal Chemistry of Drugs Acting on Central and Peripheral Opioid Receptors DA000528-05 Medicinal Chemistry of Drugs Acting on CRH Receptors DA000529-05 Medicinal Chemistry of Hallucinogenic Drugs DA000530-05 Medicinal Chemistry of Drugs Acting on Toll-like Receptors DA000531-05 Medicinal Chemistry in the Study of Alcohol Abuse DA000532-05 Medicinal Chemistry of Drugs Acting on Biogenic Amine Receptors William F. Simonds DK043304-18 Mechanism of G Protein Beta5/ R7-RGS Protein/R7BP Complex Signal Transduction Herbert Tabor DK024961-06 Phylogeny and functional significance of glutathionylspermidine in E. coli Robert Tycko DK029061-06 Structural Studies of Alzheimer's beta-Amyloid Fibrils DK075032-04 Structural Studies of HIV-1 Proteins by Solid State NMR Reed Wickner DK024950-06 Biological Roles and Structures of Yeast Prions Tsan S. Xiao (NIAID) AI000960-06 Structural Studies of Pattern Recognition Receptors

四十位首席调查人员报告说，该设施在2012财年的70个联邦研究项目中得到了支持。在这46个项目中（67％）和26个PI（67％）来自NIDDK，24个项目和14个PI来自NIH的其他地区（NIAID 12/7，NIDA 7/2，NIDA 7/2，NCI 2/2，Ninds 1/1/ 1，NIDCR 1/1，NIAAA 1/1）。 尽管在某些方面有用的指标，但PI计数和项目计数是过分的。重要的是要注意，为每个项目提供的支持量差异很大。例如，NIDDK的小分子化学家的支持以及在NIDDK（Dean，Kopp，Liang）涉及生物聚合物分析的许多项目非常涉及，而向非NIDDK实验室提供的大多数支持涉及一个或两个低的员工时间实验。肯纳·赖斯（Kenner Rice）（NIDA）的支持是一个特殊情况，因为他最近才从NIDDK搬到NIDA，并且与该设施的所有历史形式都有长期的工作关系。 请参阅此处索引的个人研究报告，以了解有关设施支持和出版物细节的重要研究的更多信息。注意：带有星号的Pi的PI在可用于自我报告的短时间内无法使用，并且在此包括在内，因为已在2012财年的这些PI中存档文件，并且他们没有对信息请求的否定作出响应，并且他们有历史记录报告他们的使用 - 只有一些未明确报告的人都包括在内。 9月30日以后的某个时候，应从D. Eric Anderson提供完全自我报告（和更完整的）上市。由于上一财政年度的报告信息削减和粘贴的习惯，索引编号通常被误报道。 丹尼尔·阿佩拉（Daniel Appella） DK031123-08 p53的小分子激活剂 DK031124-08小寡聚对折叠RNA的选择性识别 DK031143-08化学修饰的肽核酸 克利夫顿·巴里（Niaid） AI000783-15探索非复制和耐药性结核的代谢 AI000693-19开发结核病的化学治疗药 ad bax DK075023-04 HIV1 GP41外套蛋白的结构研究 DK069017-05血凝素膜融合结构域的研究 DK029047-06α-核蛋白的结构和膜结合 爱德华·伯杰（Niaid） AI000538-24 HIV受体相互作用和相关的抗HIV策略 哈里斯·伯恩斯坦 DK052037-06细菌自动转运蛋白的生物发生 Carole Bewley DK031135-05发现天然产品和天然产品样抑制剂 DK032103-12 HIV-1细胞融合的抑制剂和探针 苏珊·布坎南（Susan Buchanan）* DK011011-05来自耶尔森氏菌的外膜蛋白的结构表征 DK036139-06细菌分泌通道的结构表征 DK036143-04人类转铁蛋白的铁吸收的结构表征 Rafael Camerini-otero DK052034-07 SPO11在哺乳动物减数分裂中的作用 DK052033-07减数分裂涉及的不同途径 哺乳动物的重组 Marius Clore DK029038-05 GP41的设计抗HIV抗体 DK029023-21 NMR溶液中大分子的结构和动力学 Jurrien Dean* DK015603-06卵母细胞的分子生物学 弗雷德里克·迪达* DK036154-05新型原核DNA转座酶的结构和功能 霍华德·菲尔（NCI） BC011101-05鉴定新的神经胶质瘤相关肿瘤抑制剂和癌基因 Marvin Gershengorn DK011005来定义甲状腺激素释放激素（TRH）（TRH-RS）和甲状腺刺激激素（TSH-R）的受体的结构功能关系，其长期目标是开发针对此类探针/药物的探针/药物受体并描绘这些受体的信号传导机制 DK047044以发现低分子量配体（激动剂和拮抗剂）用于甲状腺刺激激素（TSH-R）的受体和甲状腺蛋白释放激素（TRH），其长期目标是开发针对这类受体受体/药物的长期目标 Reinhard K Grisshammer*（Ninds） NS003103-04积分膜蛋白的功能 Udayan Guha（NCI）（技术援助） BC011410-02突变EGFR激酶下游蛋白磷酸化 Deborah M Hinton DK057802-25噬菌体T4基因表达 佩吉·赫西（Peggy Hsieh） DK052029-06对DNA损伤的细胞反应 詹姆斯·赫利（James Hurley） DK036123-06 ESCRT复合物的货物排序和囊内囊泡发芽 DK036125-06 HIV-1萌芽中ALIX和ESCRT复合物的结构研究 DK036126-06泛素结合结构域的结构和功能研究 DK036127-06二酰基甘油通过C1域蛋白的机制 DK036128-06逆行蛋白质流量到高尔基的结构机制 肯·雅各布森 DK031117-24在作用于腺苷受体的药物的开发 DK031127-05在离子通道作用的药物的开发 DK031115-28生物活性化合物的功能化同源物 DK031116-24 P2Y受体配体的开发 Alan R. Kimmel DK075040-03控制生长和发育的信号通路 杰弗里·科普 DK043308-17局灶性节段性肾小球硬化：遗传学和机制 帕瓦尔·科瓦克（Pavol Kovac） DK059701-39碳水化合物的反应和免疫化学 斯蒂芬·莱普拉（NIAID） AI000929-10炭疽疫苗和治疗剂 AI001030-05分子遗传学和炭疽病的发病机理 AI001031-05炭疽芽孢杆菌的毒力因子的结构和功能 AI001032-05炭疽毒力决定因素的病理生理作用 迈克尔·莱纳多（Niaid） AI000565-16涉及核因子KAPP的信号通路的调节 自身免疫增生性综合征的AI000769-16分子机制 T. Jake Liang DK054504-16 HCV感染和HCV宿主相互作用的细胞系工和动物模型 卡罗尔·朗（Niaid） AI001020-06宿主对疟原虫抗原的免疫反应 汉斯·卢克克（Hans Luecke） DK059702-06选择性组蛋白乙酰转移酶调节剂的合成和表征 DK059703-06组蛋白乙酰转移酶信号网络的化学遗传阐明 Aleksandra Nita-Lazar（NIAID） - （仅帮助数据处理） AI001084-04涉及细胞信号的蛋白质修饰 艾米·纽曼（NIDA） DA000389-17单胺转运蛋白的新型探针 凯文·奥康奈尔（Kevin O'Connell） DK024151-10中心体成熟和重复秀丽隐杆线虫胚胎 Sushil Rane DK055106-06转化糖尿病和肥胖症中的生长因子β超家族信号 肯纳·赖斯（NIDA，曾经NIDDK） DA000527-05作用在中央和外围阿片受体上的药物的药物化学 DA000528-05作用于CRH受体的药物的药物化学 DA000529-05致幻药的药物化学 DA000530-05作用于收费受体的药物的药物化学 DA000531-05酗酒研究中的药物化学 DA000532-05作用于生物胺受体的药物的药物化学 威廉·西蒙兹（William F. Simonds） DK043304-18 G蛋白β5/ R7-RGS蛋白/ R7BP复合信号转导的机理 赫伯特·塔博尔（Herbert Tabor） DK024961-06的系统发育和谷胱甘肽在大肠杆菌中的功能意义 罗伯特·泰克（Robert Tycko） DK029061-06阿尔茨海默氏β-淀粉样蛋白原纤维的结构研究 DK075032-04固态NMR对HIV-1蛋白的结构研究 里德·威克纳（Reed Wickner） DK024950-06酵母菌prions的生物学作用和结构 Tsan S. Xiao（Niaid） AI000960-06模式识别受体的结构研究