Advanced Mass Spectrometry Facility

先进的质谱设备

• 批准号: 8350162
• 负责人: David Anderson
• 金额: $ 108.52万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8350162
• 关键词: 1,2-diacylglycerol; Alcohol abuse; Alzheimer' s Disease; Amyloid beta-Protein; Anabolism; Animal Model; Anthrax disease; Antiviral Agents; Apoptosis; Area; BRCA1 gene; Bacillus anthracis; Bacteriophage T4; Binding; Biochemical; Biogenesis; Biogenic Amine Receptors; Biological; Biological Factors; Biopolymers; Caenorhabditis elegans; Calibration; Candidate Disease Gene; Capsid Proteins; Carbohydrates; Cell Culture Techniques; Cell fusion; Cell physiology; Centrosome; Chemoprevention; Chromatin; Clathrin Adaptors; Complex; Core Facility; Corticotropin-Releasing Hormone Receptors; DAG/PE-Binding Domain; DNA; DNA Damage; Data; Data Analyses; Development; Diglycerides; Drosophila genus; Drug resistance; Dynamin; Embryo; Endocytosis; Endoplasmic Reticulum; Epidermal Growth Factor Receptor; Escherichia coli; Event; Federal Government; GTP-Binding Proteins; Gene Activation; Gene Expression; Genetic; Golgi Apparatus; Growth and Development function; Guanosine Triphosphate Phosphohydrolases; HIV; HIV Antibodies; HIV Envelope Protein gp120; HIV-1; Hallucinogens; Hemagglutinin; Hepatitis C; Hepatitis C virus; Human; Immune system; Immunochemistry; Individual; Investigation; Ion Channel; Iron; Label; Learning; Ligands; Mammals; Mammary Neoplasms; Mass Spectrum Analysis; Mature T-Lymphocyte; Measurement; Measures; Meiosis; Meiotic Recombination; Membrane; Membrane Fusion; Membrane Proteins; Metabolism; Metric; Mismatch Repair; Mitochondria; Molecular; Molecular Biology; Molecular Chaperones; Molecular Genetics; Molecular Structure; Molecular Weight; Monoclonal Antibodies; Morphology; NF-kappa B; National Human Genome Research Institute; National Institute of Allergy and Infectious Disease; National Institute of Child Health and Human Development; National Institute of Dental and Craniofacial Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Drug Abuse; National Institute of Mental Health; National Institute on Deafness and Other Communication Disorders; Neurobiology; Non-Insulin-Dependent Diabetes Mellitus; Nuclear; Obesity; Oocytes; Opioid Receptor; Organelles; Pathogenesis; Pathway interactions; Pattern recognition receptor; Peptide Fragments; Peptide Nucleic Acids; Peripheral; Pharmaceutical Chemistry; Pharmaceutical Preparations; Phosphoric Monoester Hydrolases; Phosphorylation; Phosphotransferases; Phylogeny; Play; Polyamines; Principal Investigator; Problem Solving; Protein Tyrosine Kinase; Protein p53; Proteins; Publications; Purinergic P1 Receptors; RGS Proteins; RNA Folding; Reaction; Regulation; Regulator Genes; Reporting; Research; Research Project Grants; Resolution; Resources; Rice; Role; Running; SPO11 gene; Sampling; Science; Signal Pathway; Signal Transduction; Simplexvirus; Small RNA; Software Tools; Solutions; Sorting - Cell Movement; Spermidine; Structure; Synthesis Chemistry; System; TLR8 gene; Techniques; The Sun; Therapeutic; Time; Toll-like receptors; Transferrin; Transposase; Tuberculosis; Tubular formation; Tumor Suppressor Proteins; Ubiquitin; United States National Institutes of Health; Vesicle; Viral; Virulence; Virulence Factors; Virus; Work; Yersinia pestis; alpha synuclein; chemical genetics; chromatin remodeling; crosslink; dalton; design; drug development; experience; functional genomics; histone acetyltransferase; host cell factor C1; indexing; inhibitor/antagonist; interest; lipid transport; macromolecule; mass spectrometer; molecular recognition; mutant; neoplastic cell; oral bacteria; protein complex; protein folding; protein kinase D; protein transport; receptor; research study; response; sialic acid binding Ig-like lectin; small molecule; solid state nuclear magnetic resonance; sugar; therapeutic vaccine; tool; uptake; yeast prion

Principal Investigators reported that during the 2011 fiscal year 83 federal research projects overseen by 47 principal investigators were supported by the facility. Of this total 54 projects (65%) and 31 PI's(66%) were from NIDDK, 29 projects and 16 PI's were from other parts of NIH (NIAID 14-8,NIDA 6-1,NCI 4-2, NICHD 2-2, NIMH 1-1, NIDCD 1-1). Although useful metrics for some things the PI-count and project-count are an oversimplification. It is important to note that the amount of support provided to each project varies widely. For instance support of small molecule chemists at NIDDK and a number of projects involving biopolymer analysis at NIDDK were very involved while most of the support provided to non-NIDDK labs involved one or two low staff time experiments. Support of Kenner Rice (NIDA) is a special case as he only recently move to NIDA from NIDDK and has had a long-standing working relationship with the facility in all its historical forms. Please see the individual research reports indexed here to learn more about the important research it supports and publication details. Daniel Appella DK031123-07 Small Molecule Activators of p53 DK031124-07 Selective Recognition of Folded RNA by Small Oligomers DK031143-07 Chemically Modified Peptide Nucleic Acids Ettore Appella (NCI) BC011197-02 Reg. and Func. of WIP1 Phosphatase and its Role in Tumor Cells BC005599-26 Tumor Suppressor Protein, p53 BC009101-18 Design of Antiviral Drugs Effective Against the HIV-1 Virus Clifton Barry (NIAID) AI000693-19 Development Of New Chemotherapeutics For Tuberculosis AI000783-15 Exploring the Metabolism of Non-replicating and Drug-resistant TB Edward A. Berger (NIAID) AI000538-24 Interaction of HIV with Recep. Ad Bax DK075023-03 Structural Study of the HIV1 gp41 Coat Protein DK029047-05 Structure and Membrane Binding of alpha-synuclein DK069017-04 Study of Hemagglutinin Membrane Fusion Domain Carole Bewley DK031135-05 Discovery of Nat. Products and Natural Product-Like Inhibitor DK032103-12 Inhibitors and Probes of HIV-1 Cell Fusion Leslie Baier DK069071-15 Structural Analysis of Candidate Genes for Type 2 Diabetes and Obesity Harris Bernstein DK052037-05 Biogenesis of Bacterial Autotransporter Proteins Susan Buchanan DK011011-04 Structural Characterization of Outer Membrane Proteins from Yersinia pestis DK036139-05 Structural Characterization of Bacterial Secretion Channels DK036143-04 Structural Characterization of Iron uptake from Human Transferrin Rafael Camerini-Otero DK052031-05 The role of Spo11 in Mammalian Meiosis DK052033-05 The Different Pathways Involved In Meiotic Recombination in Mammals David J Clark (NICHD) HD008775-07 Chromatin Remodeling and Gene Activation Marius Clore DK029038-05 Design of gp41 Directed anti-HIV antibodies DK029023-21 Structure and Dynamics of Macromolecules in Solution by NMR David Davies DK036157-05 Toll-like Receptors of the Innate Immune System: Human TLR8 and TLR9 Jurrien Dean DK015603-05 Molecular Biology of the Oocyte Chuxia Deng DK056009-05 Chemoprevention and Therapeutic Treatment of BRCA1 Associated Mammary Tumors Frederick Dyda DK036153-05 Structure and Function of Eukaryotic DNA Transposases Gregory G Germino DK75042-02 The Role of PKD Proteins in Regulating Tubular Morphology Udayan Guha (NCI) BC011410-01 Protein Phosphorylation Downstream of Mutant EGFR Kinases Jenny E Hinshaw DK060100-16 Structure and Function of Dynamin, a 100kd GTPase Involved in Endocytosis DK060107-05 Structural Analysis of Dynamins Involved in Mitochondrial Morphology Deborah Hinton DK057802-24 Bacteriophage T4 Gene Expression Peggy Hsieh DK052029-05 Cellular Responses to DNA Damage DK052030-05 DNA Mismatch Repair James Hurley DK036123-05 Cargo Sorting and Intralumenal Vesicle Budding by the ESCRT Complexes DK036125-05 Structural Studies of Alix and ESCRT Complexes in HIV-1 Budding DK036126-05 Structural and Functional Studies of Ubiquitin Binding Domains DK036127-05 Mechanisms of Diacylglycerol Signaling Through C1 Domain Proteins DK036128-05 Structural Mechanisms in Retrograde Protein Traffic to the Golgi DK036130-05 Molecular Recognition by Clathrin Adaptors Kenneth Jacobson DK031117-24 Development Of Drugs Acting At Adenosine Receptors DK031127-05 Development of Drugs Acting at Ion Channels DK031115-28 Functionalized Congeners Of Bioactive Compounds DK031116-24 Development Of P2Y Receptor Ligands Judith Kassis (NICHD) HD005003-12 Control Of Gene Expression During Development Alan R. Kimmel DK075040-03 Signaling Pathways in Control of Growth and Development Paul Kovac DK059701-38 Reactions and Immunochemistry of Carbohydrates Thomas Kristie (NIAID) AI000711-18 HCF-1, A Mammalian Factor Controlling HSV Gene Expression Peter Kwong (NIAID) AI005022-10 Neutralization Mechanisms of Anti-HIV-1 Monoclonal Antibodies Elissa Lei DK015602-05 Chromatin Insulator Function and Nuclear Organization Michael Lenardo (NIAID) AI000565-15 Regulation of Signalling Pathways Involving Nuclear factor kappa B AI000566-15 Gene Regulatory Events In Establishing Mature T cell Tolerance AI000718-17 Molecular Pathways of Programmed Cell Death And Viral Cytopathicity Stephen H. Leppla (NIAID) AI000929-09 Vaccines and Therapeutics for Anthrax AI001030-04 Molecular Genetics and Pathogenesis of Anthrax AI001031-04 Structure and Function of Virulence Factors of Bacillus anthracis AI001032-04 Pathophysiological Actions of Anthrax Virulence Determinants Jake Liang DK054504-15 Cell Culture and Animal Models of HCV infection and HCV-host Iinteraction Hans Luecke DK059702-05 Synthesis and Characterization of Selective Histone Acetyltransferase Modulators DK059703-05 Chemical Genetic Elucidation of Histone Acetyltransferase Signaling Networks Danial Masison DK024953-05 Yeast Prions and Hsp104 Chaperone Howard Nash/David Sandstrom (NIMH) MH002228-26 Genetic Neurobiology Of Drosophila Kevin O'Connell DK024151-09 Centrosome Maturation and Duplication in the C. elegans Embryo. Brian Oliver DK015600-16 Drosophila Functional Genomics William Prinz DK060004-10 Intracellular Lipid Trafficking and Organelle Biogenesis DK060105-05 Biogenesis of the endoplasmic reticulum Kenner Rice (NIDA, once NIDDK) DA000527-04 Medicinal Chemistry of Drugs Acting on Central and Peripheral Opioid Receptors DA000528-04 Medicinal Chemistry of Drugs Acting on CRH Receptors DA000529-04 Medicinal Chemistry of Hallucinogenic Drugs DA000530-04 Medicinal Chemistry of Drugs Acting on Toll-like Receptors DA000531-04 Medicinal Chemistry in the Study of Alcohol Abuse DA000532-04 Medicinal Chemistry of Drugs Acting on Biogenic Amine Receptors Pam Schwartzberg (NHGRI) HG000123-14 Genetic and Biochemical Approaches to Tyrosine Kinase Function William F. Simonds DK043304-18 Mechanism of G Protein Beta5/ R7-RGS Protein/ R7BP Complex Signal Transduction Peter Sun (NIAID) AI000880-11 Recognition Of HIV Envelope gp120 by Siglec Receptors Herbert Tabor DK024961-05 Phylogeny and Functional Significance of Glutathionyl-spermidine in E. coli DK024709-30 Polyamine Biosynthesis and Molecular and Cellular Functions John Thompson (NIDCR) DE000341-30 Regulation of Sugar Transport and Metabolism in Oral Bacteria Rob Tycko DK029061-05 Structural Studies of Alzheimers' beta-Amyloid Fibrils DK029063-05 Investigations of Protein Folding by Solid State NMR DK075032-03 Structural Studies of HIV-1 Proteins by Solid State NMR Reed Wickner DK024950-05 Biological Roles and Structures of Yeast Prions Tsan S. Xiao (NIAID) AI000960-06 Structural Studies of Pattern Recognition Receptors

首席调查人员报告说，在2011财年83个联邦研究项目由47位首席调查人员监督。在这54个项目中（65％）和31个PI（66％）来自NIDDK，29个项目和16个PI来自NIH的其他地区（NIAID 14-8，NIDA 6-1，NCI 4-2，NICHD 2-，NICHD 2-- 2，NIMH 1-1，NIDCD 1-1）。 尽管在某些方面有用的指标，但PI计数和项目计数却过于简化。重要的是要注意，为每个项目提供的支持量差异很大。例如，NIDDK的小分子化学家和NIDDK的许多涉及生物聚合物分析的项目的支持非常涉及，而提供给非NIDDK实验室的大多数支持涉及一个或两个低的员工时间实验。 Kenner Rice（NIDA）的支持是一个特殊情况，因为他最近才从NIDDK搬到NIDA，并且与该设施的所有历史形式都有长期的工作关系。 请参阅此处索引的个人研究报告，以了解有关它支持的重要研究和出版物细节的更多信息。 丹尼尔·阿佩拉（Daniel Appella） DK031123-07 p53的小分子激活剂 DK031124-07小寡聚对折叠RNA的选择性识别 DK031143-07化学修饰的肽核酸 Ettore Appella（NCI） BC011197-02 Reg。和功能。 WIP1磷酸酶及其在肿瘤细胞中的作用 BC005599-26肿瘤抑制蛋白，p53 BC009101-18针对HIV-1病毒有效的抗病毒药物设计 克利夫顿·巴里（Niaid） AI000693-19开发用于结核病的新化学治疗剂 AI000783-15探索非复制和耐药结核的代谢 爱德华·伯杰（Niaid） AI000538-24 HIV与Recep的相互作用。 ad bax DK075023-03 HIV1 GP41外套蛋白的结构研究 DK029047-05α-突触核蛋白的结构和膜结合 DK069017-04血凝素膜融合结构域的研究 Carole Bewley DK031135-05 NAT发现。产品和天然产品状抑制剂 DK032103-12 HIV-1细胞融合的抑制剂和探针 莱斯利·拜尔（Leslie Baier） DK069071-15 2型糖尿病和肥胖的候选基因的结构分析 哈里斯·伯恩斯坦 DK052037-05细菌自转运蛋白的生物发生 苏珊·布坎南（Susan Buchanan） DK011011-04来自耶尔森氏菌的外膜蛋白的结构表征 DK036139-05细菌分泌通道的结构表征 DK036143-04人类转铁蛋白的铁吸收的结构表征 Rafael Camerini-otero DK052031-05 SPO11在哺乳动物减数分裂中的作用 DK052033-05哺乳动物中减数分裂重组涉及的不同途径 David J Clark（NICHD） HD008775-07染色质重塑和基因激活 Marius Clore DK029038-05 GP41的设计抗HIV抗体 DK029023-21 NMR溶液中大分子的结构和动力学 大卫·戴维斯（David Davies） dk036157-05先天免疫系统的收费式受体：人类TLR8和TLR9 Jurrien Dean DK015603-05卵母细胞的分子生物学 Chuxia Deng DK056009-05 BRCA1相关乳腺肿瘤的化学预防和治疗治疗 弗雷德里克·迪达（Frederick Dyda） DK036153-05真核DNA转座酶的结构和功能 Gregory G Germino DK75042-02 PKD蛋白在调节管状形态中的作用 Udayan Guha（NCI） BC011410-01突变EGFR激酶下游蛋白磷酸化 珍妮·辛肖（Jenny E Hinshaw） dk060100-16 Dynamin的结构和功能，Dynamin，一种100KD GTPase参与内吞作用 DK060107-05与线粒体形态有关的动力学的结构分析 Deborah Hinton DK057802-24噬菌体T4基因表达 佩吉·赫西（Peggy Hsieh） DK052029-05对DNA损伤的细胞反应 DK052030-05 DNA不匹配维修 詹姆斯·赫利（James Hurley） DK036123-05货物分类和囊内囊泡发芽 DK036125-05 HIV-1萌芽中ALIX和ESCRT复合物的结构研究 DK036126-05泛素结合结构域的结构和功能研究 DK036127-05二酰基甘油通过C1域蛋白的机制 DK036128-05逆行蛋白质流量到高尔基的结构机制 dk036130-05旋转蛋白适配器的分子识别 肯尼斯·雅各布森 DK031117-24在作用于腺苷受体的药物的开发 DK031127-05在离子通道作用的药物的开发 DK031115-28生物活性化合物的功能化同源物 DK031116-24 P2Y受体配体的开发 朱迪思·卡西斯（NICHD） HD005003-12发育过程中基因表达的控制 Alan R. Kimmel DK075040-03控制生长和发育的信号通路 保罗·科瓦克（Paul Kovac） DK059701-38碳水化合物的反应和免疫化学 托马斯·克里斯蒂（Niaid） AI000711-18 HCF-1，一种控制HSV基因表达的哺乳动物因子 彼得·夸（Niaid） AI005022-10抗HIV-1单克隆抗体的中和机制 Elissa Lei DK015602-05染色质绝缘子功能和核组织 迈克尔·莱纳多（Niaid） AI000565-15涉及核因子kappa b的信号通路调节 AI000566-15基因调节事件在建立成熟的T细胞耐受性中 AI000718-17编程细胞死亡和病毒细胞病的分子途径 Stephen H. Leppla（Niaid） AI000929-09炭疽疫苗和治疗剂 AI001030-04分子遗传学和炭疽病的发病机理 AI001031-04炭疽芽孢杆菌的毒力因子的结构和功能 AI001032-04炭疽毒力决定因素的病理生理作用 杰克·梁 DK054504-15 HCV感染的细胞培养和动物模型 汉斯·卢克克（Hans Luecke） DK059702-05选择性组蛋白乙酰转移酶调节剂的合成和表征 DK059703-05组蛋白乙酰转移酶信号通信网络的化学遗传阐明 Danial Masison DK024953-05酵母菌和HSP104伴侣 霍华德·纳什（Howard Nash）/大卫·桑德斯特罗姆（David Sandstrom）（NIMH） MH002228-26果蝇的遗传神经生物学 凯文·奥康奈尔（Kevin O'Connell） 秀丽隐杆线虫胚胎中的DK024151-09中心体成熟和重复。 布莱恩·奥利弗（Brian Oliver） DK015600-16果蝇功能基因组学 威廉·普林兹（William Prinz） DK060004-10细胞内脂质运输和细胞器生物发生 DK060105-05内质网的生物发生 肯纳·赖斯（NIDA，曾经NIDDK） DA000527-04作用在中央和外围阿片受体上的药物的药物化学 DA000528-04作用于CRH受体的药物的药物化学 DA000529-04致幻药的药物化学 DA000530-04作用于Toll样受体的药物的药物化学 DA000531-04酗酒研究中的药物化学 DA000532-04作用于生物胺受体的药物的药物化学 Pam Schwartzberg（NHGRI） HG000123-14酪氨酸激酶功能的遗传和生化方法 威廉·西蒙兹（William F. Simonds） DK043304-18 G蛋白β5/ R7-RGS蛋白/ R7BP复合信号转导的机理 彼得·孙（Niaid） SIGLEC受体对HIV信封GP120的AI000880-11识别 赫伯特·塔博尔（Herbert Tabor） DK024961-05的系统发育和谷胱甘肽 - 胸腺素在大肠杆菌中的功能显着性 DK024709-30多胺生物合成以及分子和细胞功能 约翰·汤普森（NIDCR） DE000341-30口服细菌中糖运输和代谢的调节 罗布·泰克（Rob Tycko） DK029061-05阿尔茨海默氏症β-淀粉样蛋白原纤维的结构研究 DK029063-05固态NMR对蛋白质折叠的研究 DK075032-03固态NMR对HIV-1蛋白的结构研究 里德·威克纳（Reed Wickner） DK024950-05酵母prions的生物学作用和结构 Tsan S. Xiao（Niaid） AI000960-06模式识别受体的结构研究