Development of an spf Macaca nemestrina breeding colony

spf 猕猴线虫繁殖群的开发

• 批准号: 9348723
• 负责人: ROBERT J. ADAMS
• 金额: $ 97.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9348723
• 关键词: AIDS/HIV problem; Animals; Breeding; Cercopithecine Herpesvirus 1; Data; Development; Disease Management; Family suidae; Female; Funding; Genetic; Genetic Markers; Genome; Genomic approach; Genotype; Goals; Grant; Hereditary Disease; Histocompatibility; Immunogenetics; Institution; Macaca; Macaca nemestrina; Maintenance; Research; Research Personnel; Resources; SIV; Sales; Serological; Simian Retroviruses; Single Nucleotide Polymorphism; Tail; Technology; Testing; United States; United States National Institutes of Health; Universities; Viral; Virus; animal resource; functional genomics; genetic pedigree; germ free condition; health data; human disease; male; member; offspring; pig genome; research study; whole genome

This renewal of our animal resource grant (P40 OD013117) requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded in 2006 and renewed in 2011. It has the following resource component and research component aims: A. Resource Component Specific Aims: Resource Aim 1: To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. Resource Aim 2: To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. Resource Aim 3: To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. It is provided to purchasers of our animals. B. Research Component Specific Aims: To expand the genetic data available for pigtailed macaques to a) increase the research utility of pigtailed macaques and b) assist in genetic and disease management of the colony. This will be done through three research aims. Research Aim 1: To characterize pig-tailed macaque immunogenetics in detail via MHC class I and MHC class II genotyping. Research Aim 2: To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. Research Aim 3: To establish the whole genome of the pigtailed macaque using long-read sequencing technology and de novo assembly, thereby enhancing the research value of this macaque species by enabling functional genomic approaches.

我们的动物资源补助金 (P40 OD013117) 的更新需要五年的资金来继续支持 约翰·霍普金斯大学的无特定病原体 (SPF) 猪尾猕猴 (Macaca nemestrina) 群体 大学于 2006 年首次获得资助并于 2011 年更新。它拥有以下资源组成部分和 研究部分的目标： A. 资源组件的具体目标： 资源目标 1：扩大当前 SPF 繁殖群体的规模，以增加雌性数量 用于繁殖和雄性，可出售给 NIH 资助的约翰霍普金斯大学和 在其他研究机构。 资源目标 2：通过定期对 4 种病毒进行血清学和 PCR 检测来维持菌落的 SPF 状态 药剂：Cercopithecine 疱疹病毒 1（B 病毒）、猿猴免疫缺陷病毒 (SIV)、猿猴逆转录病毒 (SRV) 和猿 T 淋巴细胞病毒 (STLV)。目前，所有现有的殖民地成员都已多次 这些药物的检测呈阴性。 资源目标 3：维护所有动物的谱系和健康数据。该数据可在所有 群体中的动物，并将随着群体的成长而扩大。它提供给我们动物的购买者。 B. 研究部分的具体目标：将辫尾猕猴的可用遗传数据扩展到 a) 增加辫尾猕猴的研究效用，b) 协助猕猴的遗传和疾病管理 殖民地。这将通过三个研究目标来完成。 研究目标 1：通过 MHC I 类和 MHC 类详细表征猪尾猕猴免疫遗传学 II 基因分型。 研究目标2：通过单核苷酸多态性（SNP）遗传来表征猪尾猕猴的遗传学特征 整个猪尾猕猴基因组的标记作图。 研究目标3：利用长读长测序建立猪尾猕猴的全基因组 技术和从头组装，从而通过使该猕猴物种的研究价值得以提高 功能基因组方法。